硅胶柱

方法采用硅胶柱色谱进行分离，薄层色谱跟踪检测，HPLC检测产品纯度。

Methods Using silica gel column chromatography for separation , TLC for tracking and detection , HPLC for purity detection of the products .

通过聚酰胺及硅胶柱层析，利用不同的洗脱体系对巫山淫羊藿的化学成分进行分离得到单体化合物，然后利用UV、IR、NMR、MS等技术鉴定了化合物的结构。

The components were separated on polyamide and silica gel column chromatography and the structure was identified by spectral evidence of UV , IR , NMR and MS.

精制黄酮C水解，用乙酸乙酯萃取水解液得萃取液，萃取液减压浓缩得棕褐色固体D，固体D经硅胶柱层析得两个甙元，用波谱知识鉴定其为山奈酚、槲皮素。

Two kinds of flavone aglycones were obtained from hydrolyzed refined flavone by water , through silica gel chromatography separation .

方法：应用硅胶柱层析等分离技术分离化合物，根据理化性质及IR，MS，NMR等光谱数据鉴定结构。

METHOD : By way of spectra as IR , MS and NMR .

方法：用硅胶柱层析法对平贝总碱进行分离纯化，固定相为薄层层析硅胶G，流动相为乙酸乙酯-甲醇-氨水（17：2：1）。

The solid phase was thin layer chromatography silica gel G and the mobile phase was ethyl acetate-methanol-ammonia ( 17:2:1 ) .

方法利用硅胶柱及其他多种色谱手段对秦艽的化学成分进行分离；用UV、IR、MS、1H-NMR、13C-NMR等技术及与对照品对照进行鉴定。

Methods Various column chromatographic techniques were used for isolation and purification of the principles .

方法：大孔吸附树脂和硅胶柱色谱；IR和NMR等技术鉴定结构。

Method : The compounds were isolated by macroporous resin and column chromatography , and identified by IR , NMR data .

方法：以硅胶柱层析结合薄层制备的方法，分离白术内酯等对照品，以HPLC法测定白术中活性成分的含量。

METHODS : Sesquiterpene lactones were separated by the silica gel column and layer preparation , and the active substances were determined by HPLC .

方法HPLC法确定龙血素B及近旁杂质峰位置，硅胶柱层析用于龙血素B近旁杂质峰的分离，质谱和核磁共振用于结构鉴定。

Methods Confirm the positions of loureirin B and impurity peak of loureirin B by HPLC , the substance was isolated and purified with the silica gel column chromatography .

使用固相法合成含有不同D-型氨基酸取代的八个P物质类似物。纯化采用凝胶过滤，硅胶柱层或反相高效液相色谱。

Eight analogs of substance P ( SP ) containing various D-Amino Acid modifications were synthesised by the solid phase method , purified by gel filtration , silica chromatography combined with reversed phase HPLC .

方法通过硅胶柱色谱、聚酰胺柱色谱、SephadexLH20和制备HPLC等手段分离，利用理化性质和光谱方法鉴定。

Methods A chemical constituent was isolated by silica , polyamide , Sephadex LH-20 column chromatography and preparative HPLC .

实验结果显示，硅胶柱层析法、微波萃取法、人造纤维bluerayon吸附等方法对维生素E油中PAHs的脱除有较好的效果。

Gel column chromatography , microwave-assisted extrac - tion , concentration by synthetic fiber have good effects on the removal of PAHs in vitamin E.

方法采用硅胶柱色谱和制备性薄层色谱进行分离、纯化，IR、NMR和MS等方法进行结构鉴定。

Methods Separation and purification were conducted by silica gel column chromatographic and preparative TLC methods , the structures were identified by IR , NMR , and MS.

方法利用反复硅胶柱层析的方法对广西白背叶植物叶中的化学成分进行分离和纯化，通过IR、MS、NMR等光谱技术和化学方法鉴定化合物的结构。

Methods Silica gel column chromatography was employed to isolate and purify the constituents . Structures of the chemical constituents were elucidated by IR , MS and NMR .

该提取装置简单，由硅胶柱、活性碳柱、流量计、锥形瓶、U形管、液罐及各种连接管构成，容易组装。

This device consists of silic gel column , activated charcoal column , flowmeter , conical flask , U-tube , nitrogen jar , air pump and the connected tubes .

用SephadexG50及硅胶柱层析对其进行了纯化，得率为5‰。

It was purified by Sephadex G50 / Silica column chromatography .

A部分通过反复硅胶柱层析，SephadexLH-20纯化等方法，对其中的香豆素内酯类化合物进行了分离纯化。

Coumarin of section A was isolated through silica gel column chromatography and Sephadex LH-20 repeatedly .

方法：采用聚酰胺、硅胶柱色谱、高效液相色谱分离化学成分，UV、IR、NMR、MS等方法进行结构鉴定。

METHOD : The chemical constituents were isolated by using polyamide , silica gel column chromatography and HPLC , and the chemical structures were elucidated by UV , IR , NMR and MS.

方法：分别用硅胶柱和ODS柱采用不同的流动相对色谱峰及测定结果进行考察。

Method : To study chromatogram peak and the result with the silica gel column and ODS column with different mobile phase ;

方法青蒿经石油醚提取浓缩，浸膏经过硅胶柱，用不同配比的乙酸乙酯洗脱，用TLC法跟踪，UV法检测。

Methods Artemisinin is extracted with petroleum ether and pass column of silica gel and gradient elution with ethyl acetate , then it is tracked with TLC and detected with UV-VIS .

硅胶柱SPE-HPLC测定水产品中甲基睾酮的研究

Silica gel column SPE-HPLC determination of methyltestosterone in aquatic products

方法以生物测定为指导，采用硅胶柱、SephadexLH-20柱以及制备薄层分离板对其成分进行分离和纯化，并通过物化性质和光谱分析对其结构鉴定。

Methods Seperation and purification under bioassay-directed were performed on silica gel CC , Sephadex LH-20 CC and preparative TLC .

方法：通过分级萃取和多种硅胶柱层析（SephadexLH-20凝胶过滤、ODS反相层析和HPLC）追踪分离抗癌活性成分；

Methods : Antineoplastic compounds were screened by bioassay-guided method with partitive extraction and multiple column chromatographies ( Sephadex LH-20 , ODS and preparative HPLC ) .

方法：通过反复硅胶柱层析对黄药子氯仿部位的化学成分进行分离纯化，分别采用红外（IR）、质谱（MS）、核磁共振（包括1H-NMR，13C-NMR以及2D-NMR）方法鉴定结构；

Methods : The compounds were separated and purified by repeated column chromatography with silica gel , and identified by IR , MS , NMR and 2D NMR .

方法：利用正相、反相（RP-18）硅胶柱色谱、大孔树脂柱色谱和制备高效液相色谱分离，通过现代波谱技术（13C-NMR，1H-NMR，DEPT，EI-MS）鉴定结构。

Method : Normal , reverse phase silica gel , macroporous resin column and HPLC chromatography were used for isolation .

结果显示，提高柱温或pH，选用硅胶柱或amideC16柱，均可改善亚胺培南峰形；

It showed that the improvement of peak profile was favored by an increase of column temperature or pH , using silica gel column or Amide C 16 column .

利用AB-8大孔树脂、聚酰胺、硅胶柱层析技术结合重结晶方法对燕麦黄酮进行纯化。

The oat ethanol extractions were separated and purified by AB-8 macroporous resin , polyamide and silica gel column chromatography and recrystallization method .

方法：利用硅胶柱层析和高效液相色谱进行分离纯化，采用UV、IR、MS、NMR和2DNMR波谱技术进行结构解析。

Method : Compounds were separated and purified with silica gel column and preparative HPLC , and their structures were elucidated on the basis of spectroscopic evidence ( UV , IR , MS , NMR and 2D NMR ) .

深入研究了超临界CO2萃取得到的青蒿素粗品的硅胶柱层析提纯过程的操作条件，进一步精制以提高其纯度，来满足市场需求。

Because of the low purity of artemisinin after the supercritical carbon dioxide extraction , it was thoroughly investigated that the condition of Silica gel Column chromatography for high purity of artemisinin to meet the demand of the market .

方法：乙醇总提物经反复硅胶柱色谱，SephadexLH-20柱色谱纯化，根据理化性质和光谱数据鉴定化合物结构。

Method : Isolation and purification were carried out on the column chromatography of silica gel and sephadex LH-20.The structures were identified by spectral analysis .