电压钳技术

研究H+对非洲爪蟾卵母细胞内源性ATP受体的调制作用。采用双极电压钳技术记录卵母细胞膜ATP受体介导的电流。

To investigate the modulatory effect of H ~ + on ATP-activated current and the allosteric modulation through ATP receptor , double electrode voltage clamp technique was used to record the ATP-activated current in healthy Xenopus oocytes .

目的和方法：用双微电极电压钳技术，观察腺苷（adenosine，Ad）对绵羊心室浦肯野纤维起搏离子流1f的影响。

Aim and Methods : By using two microelectrode voltage clamp technique , to observe the effects of adenosine ( Ad ) on pacemaker current I f were examined in sheep cardiac Purkinje fibers .

把提取的鼠脑mRNA注射到卵母细胞内，在卵母细胞上以双电极电压钳技术研究某些递质的诱导电流。

Two electrodes voltage clamp technique was performed to investigate neurotransmitter induced currents after mouse brain mRNA was injected into Xenopus oocytes .

本研究用5～6周龄Sprague-Dawley大鼠，采用脊髓薄片全细胞电压钳技术记录SG神经元的膜电流。

Whole-cell voltage-clamp recordings were made on the SG neurons of 5-6 week old Sprague-Dawley rats .

应用双微电极电压钳技术，观察粉防己碱（Tet）对犬心脏浦氏纤维细胞的慢内向电流（I（？）

Effects of tetrandrine ( Tet ) on the slow inward currents in canine cardiac Purkinje fibers were studied by using two-microelectrode voltage clamp technique .

采用全细胞电压钳技术记录了豚鼠心室肌细胞ICa2＋电流，绘出了电流&电压关系曲线。

ICa 2 + was recorded in ventricular myocytes and a curve of current-voltage relationships of Ica 2 + was drawn .

采用全细胞电压钳技术的斜坡脉冲程序，观察E-4031对豚鼠心室肌细胞膜Na+/Ca2+交换电流的影响。

The effects of E-4031 on the Na + / Ca 2 + exchange current were studied in guinea pig ventricular myocytes using whole-cell voltage-clamp techniques with a ramp pulse protocol .

方法采用标准微电极及双微电极电压钳技术。

METHODS standard microelectrode and two microelectrodes voltage clamp techniques were used .

双极电压钳技术在促离子型受体研究中的应用

Application of two - electrode voltage - clamp technique in the study of the ionotropic receptor

方法：在非洲爪蟾卵母细胞表达Kv1.3cRNA通道基因，使用双电极电压钳技术记录通道电流，并用药物干预。

Method : Kv1.3 cRNA channel gene was expressed in xenopus oocytes and channel current was recorded using standard two-microelectrode voltage clamp techniques in control condition or KT intervention .

结论：成功建立了爪蟾卵母细胞表达体系，并使用双电极电压钳记录技术，对爪蟾卵母细胞表达的NMDA受体不同亚型的药理学特性进行了研究。

Conclusions : The expression system of Xenopus laevis oocytes was successfully established . Using two-electrode voltage clamp recording technique to study about pharmacological properties of expressed different sub-types of NMDA receptors .

这些表达的受体的激活电流采用双电极电压钳位技术记录。超氧阴离子自由基（SAFRs）和Aβ1-40于记录前12h、24h、96h分别加入孵育液。

Superoxide anion free radicals ( SAFRs ) and A β 1-40 was added 12 h , 24 h , 96 h to incubation solution before recording .

采用全细胞电压钳记录技术，给予选择性受体阻断剂以分离不同的突触后受体电流。

The recordings of postsynaptic currents were performed by using the whole-cell voltage-clamp method .

方法：在去除滤泡膜的非洲爪蟾卵母细胞上，建立双电极电压钳记录技术，并使用该技术观察爪蟾卵母细胞表达的内源性递质门控性和电压门控性离子通道电流。

Methods : The two-electrode voltage clamp recording technique was used to observe the ligand-gated and voltage-gated ion channel currents expressed in Xenopus laevis oocytes without follicles .

但电压钳和膜片钳技术用于鉴定毒素的结合蛋白质时具有一定的局限性，因为它们往往是定向的使用不同类型的离子通道或其亚型去探测蜘蛛毒素的活性。

However , voltage clamp and patch clamp techniques have limitations in identification of the binding proteins of a toxin because they usually directionally use different types of ion channels or their subtypes to detect the activity of a toxin .