溶血毒素

猪链球菌2型溶血毒素的产生条件及其特性分析

Effects of cultural conditions on yield of hemolytic toxin by Streptococcus suis and its characterization

方法：利用薄层层析法分析球形棕囊藻合成的溶血毒素组分，倒置显微镜下观察毒素对兔血红细胞的溶血作用；

Methods : The components of the hemolytic toxin from P. globosa in laboratory were analyzed by TLC and spraying a suspension of red blood cells ( RBCs ) .

起始pH影响嗜水气单胞菌溶血毒素活性机制的研究

Machanism of the Initial pH Affecting the Hemolysin Production of Aeromonas Hydrophila

研究从实验室培养的球形棕囊藻（Phaeocystisglobosa）提取溶血毒素的条件，探讨不同生长期球形棕囊藻溶血毒素的生成特征，用薄层色谱法对溶血成份进行了初步分析。

The extraction of haemolytic toxin from Phaeocystis globosa was studied under laboratorial condition at various algal growth phases .

胸膜肺炎放线杆菌溶血毒素特性鉴定及免疫原性研究

Characterization and Immunogenicity of Hemolytic - toxin of Actinobacillus pleuropneumoniae

大多数种类的响尾蛇分泌的毒液含有溶血毒素，会破坏血液组织功能，减弱器官功能，导致凝血障碍（中断血液凝结）。

Most species of rattlesnakes have hemotoxic venom , destroying tissue , degenerating organs and causing coagulopathy ( disrupted blood clotting ) .

目前为止，国内仅见有关球形棕囊藻的生理生态和溶血毒素特性的报道，对溶血毒素的生物合成机制方面的研究甚少。

So far , many reports on eco-physiology of Phaeocystis globosa and properties of haemolytic toxin were performed , but seldom research focused on the biosynthetic mechanism of haemolytic toxin .

溶血性毒素是有毒藻类分泌较多的一类毒素，具有溶血活性。

Many harmful algae can produce hemolytic toxin which is toxic to erythrocytes .

结论：PE能快速有效地清除蜂毒素及红细胞溶解产物，可阻断因溶血及蜂毒素导致的脏器功能衰竭，是抢救蜂螫伤危重者的有效方法。

Conclusion : Plasma exchange can quickly remove the apitoxin and lysis produce of red cells , PE can block the organ function failure caused by hemolysis and apitoxin and is an effective method for rescue severe patients by bee sting .

两种菌JA1和JA2对鲫鱼的半数致死量（LD（50））各为4.3×10~5和5.5×10~7，在两种菌培养的上清液中均有一种具有溶血活性的毒素。

The LD50 of the two strains , JA-1 and JA-2 to crucian carp were 4.3 × 105 and 5.5 × 107 respectively . There was a kind of hemolytic toxin in the supernatant of the two strains culture solution .

这45株全部能产生致死性、坏死性、溶血性的α毒素，其中35%可产生肠毒素，42.5%能形成芽孢。

It was demonstrated that all the 45 strains had the capacities to produce the lethal , necrotic and hemolytic α toxins , 35 % of them to produce the enterotoxin and 42.5 % to form spore .

副溶血性弧菌直接溶血毒素65位氨基酸突变体的构建

Mutation of the 65 ~ ( th ) AA of thermostable direct hemolysin of vibrio parahaemolyticus

目的表达和纯化融合表达的副溶血弧菌不耐热溶血毒素。

ObjectiveTo express and obtain the fused protein thermolabile hemolysin ( TLH ) of vibrio parahaemolyticus ( VP ) .

副溶血弧菌不耐热溶血毒素基因的克隆、表达及表达蛋白的复性和功能的研究

Cloning and Expression of the Thermolabile Hemolysin Gene of Vibrio Parahaemolyticus , Study on Renaturation and Functions of Its Expression Protein

目的分别制备抗副溶血弧菌及其不耐热溶血毒素（thermolabilehemolysin，TLH）的多克隆抗体，建立检测副溶血弧菌TLH的酶联免疫吸附试验（ELISA）双抗体夹心法。

Objective To prepare both polyclonal antibodies of anti-Vibrio Parahaemolyticus and anti-thermolabile hemolysin ( TLH ) for establishing the enzyme-linked immunosorbent assay ( ELISA ) double antibodies sandwich method to detect TLH .

目的探讨克隆副溶血性弧菌（Vp）耐热直接溶血毒素基因的方法。

Objective To clone thermostable direct hemolysin ( TDH ) gene ( tdh gene ) of Vibrio parahaemolyticus .

为了给建立副溶血性弧菌的免疫学检测平台的建立提供材料基础，本研究分别以重组的副溶血性弧菌溶血毒素和灭活菌体为抗原制备副溶血性弧菌特异性抗体。

In order to supply materials for establishment of the immunological detection platform , in this study , recombinant hemolytic toxins of Vibrio parahaemolyticus and inactivated bacterial were used as immunogens to prepare the specific antibodies against Vibrio parahaemolyticus . 1 .

副溶血性弧菌主要存在于海水、鱼、虾、贝类等海产品中，其引起的食物中毒临床上以腹痛、呕吐、腹泻等为主要症状。溶血毒素是副溶血性弧菌的主要致病因子。

It widely distributed in seawater , fish , shrimp , shellfish and other seafoods . Cardinal symptom of food poisoning caused by Vibrio parahaemolyticus are stomachache , diarrhea , vomiting , etc. Hemolytic toxin is the most important pathogenicity factor of Vibrio parahaemolyticus .