毛蚴

结论建立的检测日本血吸虫毛蚴PCR方法灵敏、特异，具有一定的预警作用。

It 's suggested that PCR assay for detection of S.japonicum miracidium is high in sensitivity and specificity .

肝片形吸虫毛蚴：顶突呈厚壁圆环状，中央为顶腺开口，壁上有4个腺体的开口及6根短纤毛。

The miracidium of Fasciola hepatics : The apical papilla is round and thick-walled , with the opening of the apical gland at the centre . There are 4 openings of the gland body and 6 short cilia .

文中对渗透压和pH值影响毛蚴转变和存活的机理进行了讨论。

The mechanisms by which osmolarity and pH effected miracidial metamorphosis were discussed .

渗透压、pH值对日本血吸虫毛蚴体外转变成母胞蚴的影响

Effects of Osmolarity and pH on the Transformation of Schistosoma japonicum Miracidium to Mother Sporocyst in vitro

日本血吸虫虫卵毛蚴中带信号序列基因的全长cDNA序列分析

Sequence analysis of full length cDNA of Schistosoma japonicum egg miracidia genes harboring signal sequence

目的对带有信号序列的日本血吸虫虫卵毛蚴基因的全长cDNA序列进行分析。

Objective To analyze the full length cDNA sequence of Schistosoma japonicum egg miracidia genes harboring signal sequence .

方法：以血吸虫的成虫、虫卵、尾蚴DNA为模板，利用聚合酶链反应（PCR）对日本血吸虫编码免疫原性毛蚴抗原的5D基因进行扩增，检测其敏感性与特异性。

METHODS : A PCR protocol was designed to detect the Schistosoma japonicum 5D gene encoding an immunogenic miracidial antigen in cercariae 、 eggs and adult worms .

采用可溶性虫卵抗原对粪孵毛蚴在5只以下30克粪的68例进行检测，ABC法的检出率可由ELISA的95.6%提高到100%；

Using SEA , the positive rate was 100 % in ABC-ELISA , and 95.6 % in routine ELISA among 68 cases with less than 5 miracidia in stool hatching examination .

以多只毛蚴感染多只钉螺后，逐周取样30只，以抗血吸虫卵的MG2McAb，应用ELISA检测螺体内血吸虫抗原，并同时以镜检为对照。

After snails were infected individually by exposure to many miracidia , 30 of them were detected with MG 2 McAb ELISA every week for schistosome antigen and with microscopical examination for comparison .

沼气池粪样年均寄生虫卵沉降率为99.6%，灭活率为99.8%，血吸虫卵在沼液中15d后不能孵出毛蚴；

The faecal parasite eggs can be reduced by 99 . 8 % . No miracidium hatching of schistosome eggs was found in 15 days of retention in biogas tanks .

分别拍摄共培养1h、12h、24h及3d毛蚴生长照片。将培养3d的母胞蚴注射于阴性钉螺头足部，80d后观察钉螺逸蚴情况。

The pictures of miracidia were recorded after co-cultivation for 1 h , 12 h , 24 h and 3 d. The co-cultivated for 3 days mother sporocysts injected in the head and foot of negative snails , after 80 days , observed whether cercariae could be obtained or not .

结果以其检测结果与粪便毛蚴孵化法相比，阳性符合率分别为100%、100%和83%，3种方法间无显著差异。

There is no distinct difference among the three method .

虫卵进入淡水，孵化成毛蚴。

Eggs that reach fresh water hatch into ciliated miracidia .

埃及血吸虫毛蚴表皮板的观察

Observations on the epidermal plates of miracidia of Schistosoma haematobium

日本血吸虫病快速粪便毛蚴孵化法检测研究

Study on fast hatching test for determination of Schistosoma japonicum miracidium in stool

结论日本血吸虫虫卵中毛蚴抗原SjMP10融合表达获得成功。

Conclusion The miracidial antigen from eggs of Schistosoma japonicum was expressed successfully .

低密度毛蚴感染单只钉螺室内实验研究

Study on single Oncomelania Snail infected with low density of Schistosoma japonicum miracidia in laboratory

少量血吸虫毛蚴多次感染钉螺的现场实验

Field study on the infection of Oncomelania snails repeatedly exposed to small numbers of Schistosoma japonicum miracidia

虫卵孵出的毛蚴能感染钉螺，逸出的尾蚴能再感染姬鼠和小白鼠。

The miracidia can infect Oncomelania snails and the cercariae enable Apodemus agrarius and mice to develop infection .

用不同时龄尾蚴感染雏鸭，以三角烧瓶顶管法孵化毛蚴检测雏鸭感染率。

Ducklings were infected by cercariae with different ages and examined for the infection by miracidia hatching method .

抗体可能通过阻塞卵孔或者结合特定抗原，阻止卵内毛蚴发育，达到抗卵胚发育的效果。

Antibody may occlude pore of eggs or combine special antigen to impede development of miracidia or egg maturation .

这些毛蚴进体螺体，繁殖和发育对人类有传染性的尾蚴。

These organisms penetrate into snails , multiply , and develop into cercariae , the infective form for man .

结果在20℃时日本血吸虫毛蚴的期望寿命为10.11h.最长存活时间为38h；

Results At 20 ℃, the expected life span of miracidia was 10.11h , and the longest survival time was 38 hours .

通过显微镜观察测知的只能通过显微镜观察或化学分析才能发现的，如微量的血样血吸虫毛蚴显微镜和烧瓶查病结果分析

Detectable only by microscopic examination or chemical analysis , as a minute blood sample . SCHISTOSOMIASIS DIAGNOSIS BY USING MIRACIDIUM MICROSCOPE AND FLASK

目的观察水牛感染血吸虫后排卵量和毛蚴出孵规律及其消失时间。

Objective To observe the pattern and quantity of egg excretion and the time of no egg excretion from faeces after water buffalo was infected with Schistosoma japonica .

方法采用收集新鲜成熟兔肝虫卵孵化毛蚴，分组、定时、整群在解剖镜下观察的方法，对毛蚴在20℃和25℃有光照的两种状态水体中的存活情况进行实验观察。

Methods Miracidia were hatched from eggs of fresh / mature liver of the infected rabbit , then divided into two groups and observed every two hours at 20 ℃ and 25 ℃, respectively .

三峡建坝后长江江苏段水位变化对血吸虫病流行影响的研究Ⅲ.不同水深对血吸虫虫卵孵化、毛蚴感染钉螺的影响

Impact of variation in water level of the Yangtze River after the construction of the Three Gorge Dam on the transmission of schistosomiasis in jiangsu , china ⅲ . experimental observation of impact of submergible depth on schistosome egg hat

我们在四川省眉山县血吸虫病流行区采用粪便血吸虫毛蚴孵化法检出日本血吸虫感染的7～11岁患儿34例，按性别年龄以14与同一地区的136例非感染儿童进行配对观察。

To investigate the impact of chronic infection with schistosoma on children ′ s growth and physical development , the authors conducted this comparative study on children aged 7 ~ 11 years in a schistosomiasis endemic area in Meishan county , Sichuan province .

结果Fe3+、Mg2+、Al3+对日本血吸虫毛蚴趋向的影响为显著吸引作用；Fe2+、Ca2+为排斥作用；

Results Fe ~ 3 + , Mg ~ 2 + , Al ~ 3 + solutions had obvious suction effect on the chemotaxis of miracidia of Schistosoma japonicum respectively , but Fe ~ 2 + , Ca ~ 2 + solutions had repulsion effect .

螺宿主对吸虫幼虫期的血细胞反应主要是在毛蚴入侵早期以及子胞蚴逸离母胞蚴的早期，表明螺宿主血细胞反应可能具有对幼虫发育期的特异性。

We found that the larval parasites were surrounded by blood cells of the snails especially on early stages of mother sporocyst and daughter sporocyst but not for conspicuous encapsulation reaction , which suggest that the reaction may be stage specific to development of larval trematode .