延胡索酸酶

温度≤35℃，pH6.0～7.8.菌体经过10批次转化反应后，其延胡索酸酶活性仍很稳定，酶活保存率达87.0%。

A temperature lower than or equal to 35 ℃ and an acidity of pH 6 0 ~ 7 8 After the tenth batch conversion reaction , the cell fumarase has a fairly stable acitivity showing a preservation rate up to 87 0 %

凝聚和絮凝对收集富含延胡索酸酶活微生物影响的研究

Study on the Effect of Flocculating and Coagulating Reagents on High Fumarase Producing Microorganism

L-苹果酸产生菌F-871变株合成延胡索酸酶的研究

Studies on Synthesis of fumarase by mutant Aspergillus WENTII f-871

采用壳聚糖作絮凝剂收集富含延胡索酸酶活力的产氨短杆菌MA2、黄色短杆菌MA3。

B. ammoniagenes MA 2 and B. flavum MA 3 were collected by using chitosan as flocculant .

无机凝聚剂氯化钙和高分子絮凝剂壳聚糖分别对富含延胡索酸酶的产氨短杆菌MA-2、黄色短杆菌MA-3进行凝聚和絮凝实验，两菌酶活回收率达959%～987%

The fumarase producing cells such as B.ammoniagenes MA-2 and B. flavum MA-3 were treated with inorganic coagulating reagent calcium chloride and high polymeric flocculating reagent chitosan respectively , and the recovery of the enzyme activity reached 95 9 % ~ 98 7 % .

在固定化介质中添加1.5%多胺试剂，可以提高延胡索酸酶活性50%。

The fumarase activity was increased by 50 % when 1.5 % polyamino was added to the immobilized cell solution .

一株表达延胡索酸水合酶的基因工程菌的构建

The Construction of an E.coli Strain with Expression of Fumarate Hydratase