孔细胞
- porocyte
![孔细胞](../cache/1186781.jpg)
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此方法在96孔细胞培养板上进行操作,通过GFP检测定量半数病毒复制抑制来替代通过细胞染色定量半数CPE抑制。
This method was carried on 96-well cell culture plate , quantifying the half virus replication reduction by quantifying GFP was used to replace quantifying the half CPE reduction by cell dyeing .
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方法28例临床肺部肿瘤标本制备成组织细胞悬液,在体外(96孔细胞培养板内)培养,24h后加入化疗药物。
Methods 28 specimens of lung carcinoma tissues were made into cell suspension and then cultured into 96 well cell culture board . After 24 hours , 5 kinds of anticancer drugs were put into the cell culture board .
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将小鼠5d龄ES-D3细胞源的类胚体(EBs)单细胞,按5×104/mL接种6孔细胞培养板,在DMEM基础培养基中使EBs单细胞贴壁培养。于第14!
The cells of 5-day embryoid bodies ( EBs ) derived from murine ES-D3 lineage were inoculated into each well of 6-well culture plate at 5 × 104 / mL in DMEM .
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用有限稀释法将阳性孔细胞进行克隆(重复三次)。
The positive cells were cloned by limiting dilutions ( three times ) .
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精孔细胞和卵胶膜源于滤泡细胞。
The micropylar cell and the stick membrance of the egg are derived from the follicular cells .
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根据测得的频率,将适量的噬菌体接种于96孔细胞培养板上进行细菌培养扩增。
On the basis of this frequency , a proper dose of phage was inoculated into 96 hole cell culture plate and was amplified by bacteria culture .
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细胞色素C的释放是线粒体外膜通透性增高的结果。线粒体膜通透性转换孔与细胞凋亡
Release of cytochrome C is the result of a perturbation of mitochondrial membrane permeability . Mitochondrial permeability transition pore and apoptosis
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在倒置显微镜下观察加药与未加药孔K562细胞形态。
K562 cells in test group and control group were observed by inverted microscope .
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96孔板细胞同样方法热处理,连续培养3d后,进行MTT检测。
The cells seeded in 96-well plates were preconditioned using the same method , and then cultured 3 days for MTT assay .
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用3H-TdR掺入法进行药敏实验得出的合适细胞浓度与MTS比色法中建议的细胞浓度比较,本作者取1×104/孔的细胞浓度为实验浓度;
Compared the suitable cell concentration through 3H-TdR incorporation drug sensitivity test with the suggestion concentration of the MTS colorimetry , the author adopted 1 × 104 / well as experiment cell concentration .
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GM1组培养各阶段,酪氨酸羟化酶(TH)免疫反应阳性细胞数量均明显多于对照组,至体外培养2周时,每孔阳性细胞数平均可达72.8个,与对照组相比有显著性差异。
But the number of them was obviously more than that in control group at whole culture period . On the average , it reached 72 . 8 cells every well alter two weeks in vitro . There were significant difference between GMl group and control group .
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松果体毛细血管衬以厚的无窗孔内皮细胞。
The capillaries were found lined by thick non-fenestrated endothelial cells .
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皮肤表层有丰富的粘液腺孔,细胞表面具丰富的微绒毛。
There are plenty of microvilli on the surface of epidermic cells .
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线粒体膜通透性转换孔与细胞凋亡
Mitochondrial permeability transition pore and apoptosis Transforming Bridge
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珠孔塞细胞呈毛状。
The obturator cells are trichoid .
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一旦功能大孢子分化,合点和珠孔端细胞中多糖增加。
The polysaccharides in the cells of micropylar and chalazal end increases with the formation of functional megaspore .
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⑩采用四氮唑蓝比色法检测各孔软骨细胞的A值,以反映软骨细胞增殖情况。
⑩ The absorbance ( A ) value in each well was assayed with tetrazoline colorimetry as reflection of proliferation of the chondrocyte .
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而在提取前期,多糖提取率增长较快是因为溶液中多糖浓度小而桦褐孔菌细胞内多糖的浓度大,较大的浓度差形成推动力,促使粗多糖不断从细胞内溶出。
The yield of polysaccharide is growing faster in early period with large driving force because the polysaccharide concentration in solution is low , and big differs of concentration inside and outside of Inonotus obliquus cells .
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微多相聚氨酯大孔树脂吸附细胞色素c的研究
Adsorption of Cytochrome C by Multi phase Macroporous Polyurethane Resin
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科学家们过去已经展示过,外加电场可以用来推动DNA分子通过纳米孔,即细胞膜上的微孔。
Scientists have shown that an applied electric field can drive the DNA molecules through a nanopore , a tiny hole in a membrane .
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分别于照射后3、24、48h行锥虫蓝染色,平行孔内的细胞进行胰酶消化后,用Coulter计数器计算细胞数量,应用流式细胞术测定细胞周期。
At each time point as indicated after the exposure , the cells in parallel wells were trypsinized for cell counting using Coulter counter .
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目的探讨国产超声造影剂全氟显在不同机械指数(MI)超声辐照下对正常肝细胞(HL-7702)的声孔效应及细胞损伤。
Objective To investigate the sonoporation and cell killing effect on human hepatocyte ( HL-7702 ) with domestic ultrasound contrast agent ( Perfluoropropane-albumin microsphere ), induced by ultrasound in different mechanic index ( MI ) .
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DXC-200型核孔滤膜红细胞变形能力测定仪的研制及其临床应用
The DXC-200 type instrument for measuring red cell deformability using nuclepore filtration membrane and its clinical application
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方法从Wistar大鼠肝组织中分离出KCs,培养12h并调整细胞浓度为1×106/ml/孔,将细胞分为2组。
Methods KCs were isolated by collagenase perfused Wistar rats and routinely cultured in 24-well dishes for 12 h. Cells were harvested and adjusted to a concentration of 1 × 106 / ml / well and were devided into two groups .
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肾小球毛细血管的滤过屏障由三层构成:具有窗孔的内皮细胞,肾小球基底膜(GBM),高度分化的脏层上皮细胞(足细胞)及覆盖于GBM表面的足细胞足突。
The glomerular filtration barrier present in the capillary walls is comprised of a fenestrated endothelium , the glomerular basement membrane ( GBM ), and highly specialized visceral epithelial called podocytes , the foot processes of which cover the outer surface of the GBM .
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垂周壁上具有纹孔的表皮细胞;
Epidermal cells with pitted anticlinal walls and finely striated cuticle ;
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栉孔扇贝血液细胞的免疫功能
Immunological functions of blood cells in the scallop Chlamys farreri
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脑啡肽对栉孔扇贝血细胞聚集和附贴的影响
The influence of enkephalin on hemocyte aggregation and adhesion in Chlamys farreri
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可见有窗孔的内皮细胞及正常的基底膜。
Fenestrated endothelium is present , and the basement membrane is normal .
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深孔的电解加工孔内可见细胞穿行。
The Electrolyzed Processing of Deep Hole Some cells were passing through them .