大豆凝集素

大豆凝集素与肉鸡小肠黏膜细胞结合规律的研究

Binding Rule of Soybean Agglutinin to Small Intestinal Epithelia in Broiler

纯化的大豆凝集素对大鼠内脏器官发育与免疫功能的影响

Effects of Purified Soybean Agglutinin on Immune Function in Rats

用经N-乙酰基-D-半乳糖胺-epoxy-sepharose6B亲和层析体系纯化的大豆凝集素制备兔抗大豆凝集素多克隆抗体，建立大豆凝集素间接抑制ELISA定量检测方法，检测下限低于10ng/mL。

Soybean agglutinin ( SBA ) was purified with GalNac-epoxy-sepharose 6B affinity chromatography systems , and polyclonal antibody of rabbit-anti-SBA was made . The way of quantitative determination of SBA with indirect inhibiting ELISA was established and the low limit was lower than 10 ng / mL.

大豆凝集素结构及其活性测定方法的研究进展

Advances of Research on Structure and Activity Detecting Method of Soybean Agglutinin

大豆凝集素的分子结构及其抗营养作用研究进展

Advances of Researches on Molecular Structure and Its Anti-nutritional Effect of Soybean Agglutinin

正常小鼠肝细胞及腹水型肝癌细胞的大豆凝集素受体研究

Comparison of soy bean agglutinin receptors on hepatoma and normal mouse liver cells

间接抑制酶联免疫吸附法测定大豆凝集素方法的建立

Establishment of Indirect Inhibiting ELISA Method to Detect Soybean Agglutinin in the Soybean Products

胰蛋白酶对完整的大豆凝集素不产生降解作用。

Trypsin do not degrade complete SBA .

大豆凝集素在兔消化道不同部位内的免疫活性残留

Survival of Soybean Agglutinin with Immune Activity in Different Parts of Digestive Tracts in Rabbits

动物的胃蛋白酶对大豆凝集素有一定程度的降解，但降解需要一定时间。

SBA can be degraded by pepsin in certain degrees , but time is needed .

添加N-乙酰-D-半乳糖胺消除大豆凝集素抗营养活性的可行性研究

A Primary Research on the Elimination of Antinutritional Effect of Soybean Agglutinin by Adding N-acetyl-D-galactosamine

这一结果表明大豆凝集素最理想的纯化方法是N-乙酰基-D-半乳糖胺-expoxy-sepharose6B亲和层析体系。

This result indicated that the best way to purify soybean agglutinin was GalNac-epoxy-Sepharose 6B .

大豆凝集素对不同种属动物的抗营养程度和抗营养机理不尽相同。

The degrees and mechanisms of antinutritional effects of SBA are incompletely different among different species .

大豆凝集素与大鼠和小鼠小肠黏膜上皮细胞结合规律的研究

The Binding Rule of Soybean Agglutinin in the Small Intestinal Epithelia in Different Age Rats and Mice

胃蛋白酶、胰蛋白酶对大豆凝集素稳定性的影响胰蛋白酶限制性水解脱酚棉籽蛋白的研究

Study on Stability of Soybean Agglutinin to Pepsin and Trypsin ; Partially hydrolysis of de-gossypol cottonseed protein with trypsinase

在朱顶红生殖细胞的表面有前两种凝集素的受体，分布比较均一，但是没有大豆凝集素的受体。部分受精卵的第一极体有极明显的分裂现象。

Polar distribution of lectin receptors was observed on tailed generative cells . Some first polar bodies divided into two small cells .

大豆凝集素降低了膜脂酰基链和甘油主链的流动性，而花生凝集素则增强了这两个部位的流动性，这与它们的疏水性大小相关。

Soybean lectin reduced while peanut lectin increased the fluidity of fatty acyl chains and glycerol backbone , which was related with their hydrophobicity .

本文用辣根过氧化物酶标记的麦芽凝集素、大豆凝集素及花生凝集素来研究大、小鼠肝、胰中凝集素受体的分布。

The distribution of lectin receptor in liver and pancreas of rats and mice was investigated using WGA , SBA , PNA labeled with horseradish peroxidase .

鸡与兔的趋势基本一致，不过鸡的小肠黏膜对大豆凝集素的吸收能力不如兔。

The tendency of chicken is similar to rabbit , however , the ability to absorption SBA of the intestinal mucosa is lower than that of rabbit .

单胃动物中，兔对大豆凝集素吸收能力最强，表明其小肠黏膜对大豆凝集素的通透性最大，更易于引起因吸收大豆凝集素而造成的抗营养作用。

Permeability of the intestinal mucosa of rabbit is the highest in the mono-stomach animals because of its strongest absorption , so it is easy to arise the antinutritional effects caused by absorbing SBA .

大豆本身的凝集素基因作为PCR检测的内置检测标准，有效排除了由于DNA质量、实验操作等因素对检测结果的影响。

The lectin gene of soybean itself was used as internal standard to exclude the interference of DNA quality and experimental operation problems .

用N-乙酰基-D-半乳糖胺-epoxy-Sepharose6B、半乳糖胺-CH-Sepharose4B和瓜尔胶三种亲和层析体系对大豆中的凝集素进行分离提纯，并进行了多方面的比较。

Purification of soybean agglutinin with three affinity chromatography systems , GalNac-epoxy-Sepharose 6B , Gal-CH-Sepharose 4B and guar were compared .

结果表明，间接抑制ELISA检测大豆制品中的凝集素含量变异系数小于15%，样品回收率误差在15%以内；

With this standard curve , SBA contents in several kinds of soybean products were measured . The results showed the coefficent of variation of indirect inhibiting ELISA method was less than 15 % and the error of recovery rate was not more than 15 % .

本文对大豆中主要抗营养因子-蛋白酶抑制因子、大豆凝集素、植酸、大豆抗原的作用机理及对鱼类的影响进行了综述，并提出今后研究的重点和意义。

So this article discuss the main anti-nutritional factor protease inhibitor . Soybean agglutinin , phytic acid , antigenic protein action mechanism and the effects on fish and propose the next research key point and the significance .

利用改良的CTAB法，对多种大豆（GlycineMax）加工产品DNA进行分离纯化，并以大豆特异性内源基因大豆凝集素基因为参照，对用该方法获得的DNA的可扩增性加以验证。

A modified CTAB method for DNA purification from processed genetically modified soybean ( glycine max ) products ( GMO ) was adopted .

综述了大豆乳清蛋白的组成成分及生理功能，重点论述了大豆胰蛋白酶抑制剂以及大豆血球凝集素的组成及生理活性，特别论及Bowman-Birk抑制剂的抗癌活性。

The study on the components and physiological function of whey soy proteins was discussed . The main attention were paid on the composition and physiological activity of soybean trypsin inhibitor and soybean agglutinin . The anticarcinogenic activity of Bowman-Birk inhibitor was touched upon especially .