双抗体夹心法

应用双抗体夹心法ELISA检测IBDV的研究

Studies on the Detection of IBDV Using Double Antibody Sandwich - ELISA

ELISA双抗体夹心法检测FI抗原在鼠疫菌鉴定中的初步应用

Preliminary application of double antibody sandwich ELISA for detecting fi antigen in the identification of plague bacilli

单克隆抗体ELISA双抗体夹心法测定人白细胞介素2

Determination of human interleukin-2 by double antibody sandwich ELISA with monoclonal antibody

应用ELISA双抗体夹心法检测猪细小病毒抗原的研究

Detection of Porcine Parvovirus Antigen with a Sandwich ELISA

ELISA双抗体夹心法定量检测AFP。

AFP was measured by ELISA double antibody sandwiched method .

以ELISA双抗体夹心法和ELISA竞争法检测RV抗原。

The RV antigen was determined by double antibody sandwich ELISA and competitive ELISA .

并采用ELISA双抗体夹心法研究单抗的特异性和敏感性。

The specificity and sensitivity of McAbs were studied using the sandwich method of ELISA with double antibodies .

双抗体夹心法ELISA定量检测前列腺癌组织中转化性生长因子β1

An quantitative evaluation of transforming growth factor - β _1 in tissues of prostatic cancer and BPH with sandwich ELISA

结论：成功研制出抗AIB1-C单克隆抗体，为双抗体夹心法ELISA试剂盒的研制奠定基础。

Conclusion : The McAb was prepared successfully , which lays a foundation for establishing the double antibody sandwich ELISA assay .

本文叙述了测定人白细胞介素2（IL-2）含量的ELISA双抗体夹心法。

In the present experiment , a double antibody sandwich ELISA method for measurement of human interleukin-2 ( IL-2 ) was described .

采用ELISA双抗体夹心法测定其血浆GMP-140、D二聚体的含量，并以19例正常新生儿作为对照。

The levels of GMP 140 and D dimer were detected by the method of double antibodies ELISA .

方法：采用双抗体夹心法ELISA法对不同等级的高血压病患者和高血压病合并心力衰竭患者35例及30名健康者的血浆IL-18水平作了检测和比较。

Methods : Plasma levels of IL-18 in 35 patients with PH and 30 health controls were measured by ELISA .

ELISA双抗体夹心法检测耐药前后患者血清CK18水平。

Serum CK18 levels were detected by ELISA before and after drug resistance .

测定指标：采用酶联免疫双抗体夹心法原理检测血浆vWF、GMp一140水平。

The plasma level of vWF and GMP - 140 was as - sayed using ELISA .

建立了灵敏的酶标双抗体夹心法测定血清可溶性IL-2受体（sIL-2R）。

In this work , a simple and sensitive assay for soluble IL-2 receptor ( sIL-2R ) was established .

以单克隆抗体4C2作为捕捉抗体，兔多克隆抗体作为检测抗体，建立了ELISA双抗体夹心法，检测RV抗原。

A double antibody sandwich ELISA for RV antigen was developed using McAb4C2 as capture antibody and rabbit PcAb as detection antibody .

方法用ELISA双抗体夹心法分别检测健康人及OA患者HA补充治疗前后的血清及关节滑液中以上各细胞因子的含量并进行比较。

Methods Cytokine contents in serum and synovia of healthy people and of OA patients were respectively detected by ELISA before and after HA viscosupplementation .

方法采用双抗体夹心法和逆转录PCR方法，对应用干扰素治疗的23例慢性乙型肝炎患者外周血淋巴细胞中Th1/Th2细胞因子的表达水平进行动态检测。

Methods Th1 / Th2 type cytokines were detected by ELISA and RT PCR in 23 patients with chronic hepatitis B who were treated with interferon α for 24 weeks .

酶联免疫吸附试验（ELISA）双抗体夹心法测定氧化低密度脂蛋白（oxLDL）水平。

Oxidative low density lipoprotein ( oxLDL ) determination with enzyme-linked immunosorbent assay ( ELISA ) double antibody with filling method .

方法通过酶标双抗体夹心法测定53例EH患者和32例健康老人血清TNF-a水平。

Methods Seram TNF-a in 53 cases of EH and 32 cases of healthy elderly , was measured by suing ELISA .

方法应用ELISA双抗体夹心法对40名正常儿童和40例肺炎支原体肺炎急性期患儿的外周血IFN-γ和IL-4进行测定。

Methods The peripheral blood concentrations of IFN - γ and IL-4 were measured using ELISA in 40 children with Mycoplasmal pneumonia at the acute stage .

本文探讨了用ELISA双抗体夹心法，进行乙型脑炎患者血清中抗乙脑病毒IgM抗体的测定。

We have studied the application of double antibody sandwich method of ELISA for measuring specific IgM in sera of patients infected with epidemic encephalitis B virus .

制备兔抗DNA抗体，建立ELISA双抗体夹心法测定了生育、不育和流产共103对夫妇血清DNA和男性精浆DNA。

The rabit anti-DNA serum was prepared and art enzyme linked immunosorbent assay ( ELISA ) for measurement of DNA in serum and seminal plasma of 103 couples with fertility , infertility and abortion groups .

用ELISA双抗体夹心法检测人重组白细胞介素2（rHuIL-2）在酵母菌中表达的研究

Study on detection of recombinant human interleukin-2 ( rhuil-2 ) in yeast by sandwich ELISA

运用酶联免疫吸附法（单克隆双抗体夹心法）测定血浆中S100b蛋白含量。

Levels of plasma protein S 100b were measured with ELISA .

应用ELISA双抗体夹心法检测自抗体过量型至抗原过量型的系列1C模拟血清，仅抗原过量时呈阳性；

Serial artificial 1C in antibody excess and antigen excess sera were detected by sandwich ELISA . The results showed that only antigen excess sera were positive .

方法采用Elisa双抗体夹心法分别检测41例白癜风患者血清及负压吸引疱疱液IL-18和IFN-γ水平，并与正常对照组比较。

[ Methods ] IL-18 and IFN - γ in serum and suction blister fluid were detected by double-sandwich Elisa in 41 patients with vitiligo , compared with normal controls .

采用ELISA双抗体夹心法检测患颤抖病的河蟹病料，致病性病毒的阳性检出率为92.5%（37／40）。

To detect the virus causing " trembling disease " in Eriocheir sinensis samples by double antibody sandwich enzyme-linked immunosorbent assay ( ELISA ), the positive rate was 92.5 % ( 37 / 40 ) .

因此，ELISA间接法敏感性高、特异性强和重复性好，可代替ELISA间接双抗体夹心法以检测RV血清抗体。

Thus , indirect ELISA proved to possess high sensitivity , specificity and reproducibility , it can be used to replace indirect sandwich ELISA for the detection of serum antibody against RV .

方法：从人血浆中提纯的PAP作为免疫原制备特异性单克隆抗体，通过双抗体夹心法酶联免疫吸附试验（ELISA）对该单克隆抗体应用价值进行评估。

Methods : Preparing the purified PAP from fresh human plasma to be used as the monoclonal antibodies , then evaluating the value of application by the method of sandwiched ELISA .