原始卵泡

结论：1、原癌基因c-myc和neu1参与原始卵泡的启动生长发育过程，可能起正向调控作用。

Proto-oncogenes c-myc and neul possibly played a positive role in the initiation of primordial follicle growth . 2 .

原癌基因c-myc可能参与Wnt途径和Notch信号转导通路启动原始卵泡的生长发育。

The Wnt and Notch signaling pathways including proto-oncogene c-myc might be involved in the initiation of primordial follicle growth . 4 .

D组中形态正常的原始卵泡比例和初级卵泡比例均明显低于A、B、C组。

The proportions of morphologically normal primordial and primary follicles from Group D were lower than those from Group A , Group B , and Group C.

4组裸鼠卵巢原始卵泡数、生长卵泡数分别比较差异具有统计学意义（P0.05）。

The number of ovarian primordial follicles and growth follicles in 4 groups has statistically significance ( P 0.05 ) .

一旦原始卵泡闭锁或消耗过快，便会引发卵巢早衰（Prematureovarianfailure，POF），导致内分泌失调和不孕症等。

Once the depletion and atresia of primordial follicles is too fast to cause premature ovarian failure , endocrine dyscrasia and infertility .

B、C组形态正常的原始卵泡比例和初级卵泡比例，与A组相比无统计学差异。

The difference in proportions of morphologically normal primordial follicles among Group A , Group B , and Group C was not significant , so were the proportions of morphologically normal primary follicles .

结果提示：EGF在胎鼠卵巢原始卵泡生长起始中起重要作用；

The results suggested that : EGF plays an important role in the initiation of mouse fetal ovarian primordial follicles .

顺铂组原始卵泡数、生长卵泡数明显少于其余三组，差异具有统计学意义（P0.05）。

The original number of primordial and growth follicles in CDDP group is less than the other three groups , which has significantly difference ( P 0.05 ) .

敲减卵巢中原癌基因c-myc和neu1的表达后，观察原始卵泡启动生长情况。

The growth of primordial follicles was observed after knocking down the expression of proto-oncogenes c-myc and neul in the ovary .

目的：我们前期采用基因芯片技术，表明原癌基因c-myc和neu1在原始卵泡正常启动生长过程中表达上调。

Objective : In our early studies , we have shown that proto-oncogenes c-myc and neul up-regulate during primordial follicle assembly and development using the microarray approach .

其中培养12h时，0.60IU/mL组原始卵泡数量最低，组间差异显著。

0.60IU/mL group the lowest number of primordial follicles , significant differences between the groups .

结果提示，FSH虽然不是小鼠胚胎卵巢原始卵泡生长起始所必需，但对腔前卵泡的进一步发育及其雌二醇的分泌有促进作用；

The results suggested : ① FSH promotes the follicle development of mouse fetal ovarian and the secretion of estrodial , although FSH is not necessary for the initiation of primordial follicles ;

原始卵泡的启动生长和分化：原始卵泡生长启动可能受卵巢内在因子，像生长因子的调控，而不是人所想象的FSH；

Primordial follicle growth and deferentiation : the initiation of primordial folicle growth is controlled by ovarian paracrine factors , such as EGF of other growth factors , but not by FSH ;

本研究拟通过RNA干扰方法和基因芯片技术探讨原癌基因c-myc和neu1在原始卵泡启动生长中的作用及相关信号通路的初步分析。方法：构建原癌基因c-myc和neu1慢病毒干涉载体。

In this experiment , we further investigated the role and the possible related-signaling pathway of proto-oncogenes c-myc and neul in the initiation of primordial follicle growth in rats by using RNA interference and microarray methods . Methods : Lentivirus-mediated c-myc and neul RNA interference vectors were constructed .

在原始卵泡、初级卵泡、次级卵泡及成熟卵泡的颗粒细胞中都有mPGES免疫染色，其中原始卵泡中最强，初级卵泡、次级卵泡和成熟卵泡中较强。

There was detectable mPGES immunostaining in the granulosa cells of the different follicles . The strongest mPGES immunostaining was seen in the primordial follicles , while a strong level of the immunostaining was also observed in the primary follicles , the secondary follicles , and mature follicles .

敲减卵巢中原癌基因neu1后，导致与neu1信号通路相关基因Cln3在原始卵泡启动生长过程中表达发生显著变化。

After proto-oncogene neul in the ovary was knocked down , there was a significant change in the expression of genes related to neul signal pathway ( i.e. Cln3 ) during the process of the initiation of primordial follicle growth .

不同繁殖类型牦牛卵巢原始卵泡的观测

Observation of Ovary Primordial Follicle in Yaks of Different Reproduction Type

哺乳动物原始卵泡启动和生长的调控机理

Regulating Mechanism of Original Follicle Initiation and Growth of Mammalian

常见1～3个卵泡上皮细胞包围的卵母细胞形成原始卵泡；

The primary follicle often consists of 1 - 3 follicular cells .

原始卵泡形成和发育调节机制研究进展

Advance in Regulation of Primordial Follicle Assembly and Development

哺乳动物原始卵泡生长启动的调控

Regulation of Initiation of Primordial Follicle Growth in Mammals

原始卵泡能在体外启动，并得到一定程度的生长。

The primordial follicles could initiated and grow some .

从59天开始卵巢皮质深层出现少量的形态不典型的原始卵泡；

The un-typical primordial follicle was found in the ovary cortices from 59d .

原始卵泡活化的相关研究进展

Research progress in the activation of primordial follicle

12周龄原始卵泡形成类似透明带物质。

Zona pellucida-like structure was found in 12-week .

卵巢组织形态学观察及计数具有正常结构的原始卵泡及生长卵泡数。

Morphological observation and counting the number of primordial follicles and growth follicles . 5 .

在卵泡发育的早期阶段（包括原始卵泡阶段、初级卵泡阶段和小窦前卵泡阶段），闭锁是先由卵母细胞的凋亡启动的，继而是颗粒细胞的凋亡。

Atresia is started by the apoptosis of oocytes in primordial follicles and primary follicles .

前泡膜细胞被募集到原始卵泡周围，原始卵泡发育并向初级卵泡转化。

The germ line cysts break apart into individual cells and become packaged into primordial follicles .

而原始卵泡储备减少，凋亡加速，卵泡闭锁、卵泡成熟过程存在障碍也是其中之一。

One of the causes is the reduction of primordial follicle reserves , accelerated apoptosis , follicular atresia .

年老的大鼠和年幼的大鼠的卵巢组织相比，皮质减少，髓质增多，原始卵泡以及发育卵泡的数量减少。

There were thinner cortex and more medulla in older rat 's ovary as compared with young rat 's ovary .