二硫苏糖醇
- 名dithiothreitol;DTT
![二硫苏糖醇](../cache/1190316.jpg)
-
在氧化重折叠反应液中加入还原剂二硫苏糖醇(DTT)后,不但没有分子间二硫键形成,也没有异肽键形成。
No dimmers / oligomers were detected when the refolding and unfolding solution contained the reducing agent dithiothreitol ( DTT ) .
-
在精子冷冻保存液中加入二硫苏糖醇(DTT),观察复温后精子复苏率、运动速度、测定精子内ATP和精浆内GOT含量。
Dithiothreitol ( DTT ) could significantly improve the recovery and velocity of frozen-thawed spermatozoa when it was added in the protective medium .
-
二硫苏糖醇和β-巯基乙醇在SDS-PAGE电泳过程中的扩散效应
Diffusing effect of DTT and 2-ME during SDS-PAGE electrophoresis
-
目的:评价用二硫苏糖醇代替2巯基乙醇灭活IgM抗体在IgG抗A(B)测定中的应用。
Objective to evaluate the application of dithiothreitol ( DTT ) instead of 2-Mercaptoethanol ( 2-me ) in the detection of anti-type A ( or B ) IgG antibody .
-
二硫苏糖醇对小鼠运动耐力、肝组织自由基代谢、肝糖原含量及血清GPT活性影响研究
Effect of DTT on Endurance , free radical in liver tissues and activity of serum GPT in trained mice
-
针对板栗加工过程中的褐变问题,研究了沸水浴、pH值、缓冲液、维生素C、二硫苏糖醇等不同处理对板栗果实过氧化物酶和多酚氧化酶活性的影响。
According to the browning phenomenon of chestnut , the research on characteristics of the activities of peroxidase and polyphenol oxidase were performed by various treatments such as boiled water , pH , buffer solution , organic acid and DTT on ripening fruits .
-
在8mol/mL尿素溶液中,以二硫苏糖醇(DTT)为还原剂,对基因工程血管抑素3A包涵体进行溶解实验。
The inclusion bodies of 3A were dissolved in 8 mol / mL urea buffer , and dithiothreitol ( DTT ) was added as reducing agent .
-
二硫苏糖醇(DTT)对复合物中联结R&PE与APC间的脂族二硫桥键的还原导致分子内能量传递的阻断这一现象进一步证实了复合物中存在分子内能量传递现象。
The phenomena of by DTT reduced the aliphatic disulfide bridge between R-PE and APC in the conjugate further verified the intramolecular energy hansfer from R-PE to APC .
-
用光谱法研究了二硫苏糖醇(DTT)还原马脾铁蛋白(HSF)释放铁的动力学。
The kinetics of iron released from the ferric core of horse spleen ferritin ( HSF ) by dithiothreitol ( DTT ) was determined by electronic spectroscopy method .
-
方法用二硫苏糖醇还原,邻苯二醛柱前衍生,用长(150mm)或短(30mm)反相C18柱色谱分离,再用荧光检测器测定血浆GSH。
Method Reduction with dithiothreitol and Pre-column derivation with o-phthalaldehyde followed by high-performance liquid chromatographic ( HPLC ) method with a short or long reversed-phase C18 column and fluorescence detection are described .
-
采用二硫苏糖醇还原IgG,研制成99mTc-IgG一步法药盒,并与配体交换二步法标记进行对比。
The preparation of one-step kit for the 99mTc labeled human IgG with dithiothreitol reduction and a comparison of the one-step method with the ligand exchange method were reported , The stability was good , and labeling efficiency was over 95 % .
-
还原型谷胱甘肽(GSH)或二硫苏糖醇参与反应时,甲醛的累积量明显降低,表明SLO对灭害威脱甲基作用时,产生自由基。
A significant decline in the formaldehyde accumulation in the presence of either reduced glutathione or dithiothreitol suggested generation of a free radical species as an initial oxidation intermediate during the demethylation of aminocarb by SLO .
-
通过叶绿素荧光技术研究了二硫苏糖醇(1,4dithiothreitol,DTT)对大豆叶片光系统I(PSI)和光系统Ⅱ(PSⅡ)间激发能分配的影响。
Chlorophyll fluorescence technique was used to investigate the effect of 1,4-dithiothreitol ( DTT ) on the distribution of excited energy between photosystem I ( PS I ) and photosystem II ( PS II ) in soybean leaves under high irradiance .
-
在无二硫苏糖醇(DTT)存在下得到部份纯化的氧化型PFP酶,在广泛的pH范围内(pH6.0~9.0)失去其大部分对果糖2,6-二磷酸的敏感性。
The thio-dependent activation of Fru-2 , 6-P_2 to tomato fruit PFP has been investigated . The enzyme partially purified in the absence of dithiothreitol almost lost its sensitivity to Fru-2 , 6-P_2 over the range from pH 6.0 to 9.0 ( Fig. 2 ) .
-
纯化蛋白的氧化还原活性通过二硫苏糖醇还原牛胰岛素的反应得到证实。
The redox activity of the purified protein was confirmed by reduction of insulin disulfides .