二氢叶酸还原酶

二氢叶酸还原酶耐药基因对人外周血CD（34）~+细胞的保护作用

Protective effect of mutant dihydrofolate reductase gene on human peripheral blood CD _ ( 34 ) ~ + cells against methotrexate

用RT-PCR检测二氢叶酸还原酶（DHFR）mRNA表达量；

The level of dihydrofolate reductase ( DHFR ) mRNA expression was assayed with RT PCR .

目的研究二氢叶酸还原酶（DHFR，D）与谷氨酰胺合成酶（GS，G）单基因和二氢叶酸还原酶与谷氨酰胺合成酶（DHFR+GS，DG）双基因筛选扩增系统对外源基因表达的影响。

Objective To study the effect of gene amplification and selection system with DHFR plus GS and DHFR or GS gene on the foreign gene expression .

其中，嘧啶类化合物具有突出的生物活性，如抗HIV-1逆转录酶、二氢叶酸还原酶、抗肿瘤、蛋白激酶、细菌、真菌等活性。

Pyrimidine compounds possess excellent biological activities such as anti HIV-1 reverse transcriptase enzyme , dihydrofolate reductase , anti-tumor , protein kinase , bacteria , fungus and so on .

分别用绿色荧光蛋白（GFP）基因、β葡糖醛酸酶（GUS）基因以及小鼠二氢叶酸还原酶（DHFR）基因3种报告基因置换CP基因。

The CP gene was replaced by green fluorescent protein ( GFP ) gene ,β glucuronidase ( GUS ) gene or mouse dihydrofolate reductase ( DHFR ) gene , respectively .

方法：以逆转录病毒为载体，将双突变的二氢叶酸还原酶基因（DH-FR）和胞苷脱氨基酶基因（CD）转染小鼠骨髓细胞，观察该细胞耐MTX及Ara-C的CFU-GM生成情况；

METHODS : Double-mutant dihydrofolate reductase-cytidine deaminase gene was transferred into mouse bone marrow cells by retroviral vector . Colony-forming unit granulocyte-macrophage ( CFU-GM ) to MTX and Ara-C was gotten by cell culture .

目的探讨突变的二氢叶酸还原酶（mDHFR）耐药基因在人外周血CD34＋细胞中抗甲氨喋呤（MTX）的效应。

Objective To examine if human peripheral blood hematopoietic progenitor cells transduced with mutant dihydrofolate reductase ( mDHFR ) gene may increase their resistance to metrotrexate ( MTX ) .

抗原与抗体基因分别与二氢叶酸还原酶（DHFR）的两部分相连。

In this assay , two interacting proteins ( target and antibody ) are genetically fused to the two halves of the dissected enzyme dihydrofolate reductase .

新载体pET-DB对带有His-tag的仓鼠二氢叶酸还原酶的高效表达与纯化

High Level Soluble Expression and Purification of The His-tagged Chinese Hamster Dihydrofolate Reductase in E.coli Using a Newly Engineered pET-DB Vector

鸡肝二氢叶酸还原酶（DHFR）平衡态的去折叠曲线符合二态模型，但在4.0mol／L脲中的去折叠动力学为两相。

The urea-induced equilibrium unfolding transition of dihydrofolate reductase ( DHFR ) from chicken liver was well described by a two-state unfolding model involving only native and unfolded forms .

二氢叶酸还原酶（dihydrofolatereductase，DHFR，EC1.5.1.3）是核酸代谢中关键的酶之一，是癌症药物治疗中的主要靶酶，近年来得到广泛的研究。

Dihydrofolate reductase ( DHFR ) is a crucial enzyme in biochemistry . The enzyme has been the subject of extensive study because of its importance in intermediary metabolism and it is the target for a number of chemotherapeutic .

采用Lipofectin法将其转染二氢叶酸还原酶缺陷型中国仓鼠卵巢细胞（CHO-dhfr-），获得有效表达人C1-INH的CHO-dhfr+细胞克隆。

This plasmid was transfected into DHFR-deficient Chinese Hamster Ovary cells ( CHO-dhfr ) by Lipofectin method , and the positive CHO-dhfr + cell clones which stably express C1-INH was generated .

目的：为了保护骨髓免受或少受化疗药物的损伤，用转染人类突变二氢叶酸还原酶（DHFR）基因反转录病毒载体，研究其耐药特性及在小鼠造血细胞的表达和对造血细胞的保护作用。

Objective : To protect the bone marrow cells from chemotherapeutic injuries , using retroviral vector of mutant human dihydrofolate reductase gene , the paper investigated its drug resistant characteristics and for murine hematopoietic cells transduction and protection .

5-取代苯硫基（硒基）-2,4-二氨基嘧啶对大肠杆菌（MB1428）二氢叶酸还原酶抑制作用构效关系的研究

Structure-activity relationships of the inhibition of Escherichia coli dihydrofolate reductase ( mb1428 ) by 5 - ( substituted phenyl ) thio and 5 - ( substituted phenyl ) seleno-2,4-diaminopyrimidines

恶性疟原虫二氢叶酸还原酶基因的突变

Gene Point Mutation in the Dihydrofolate Reductase-thymidylate Synthase Gene of Plasmodium falciparum

二氢叶酸还原酶基因在肿瘤生物治疗中的作用

The effect of dihydrofolate reductase gene on biotherapy of cancer

中国仓鼠卵细胞二氢叶酸还原酶基因在大肠杆菌中的高效表达

High-level Expression of CHO Dihydrofolate Reductase Gene in Escherichia coli

二氢叶酸还原酶结合底物的去除

Removal of the Bound-Substrates from Dihydrofolate Reductase by Flat-Bed Isoelectric Focusing Electrophoresis

人多药耐药基因-1及二氢叶酸还原酶基因共转导小鼠造血细胞

Cotransduction of Human mdr-1 and Dihydrofolate Reductase Genes into Murine Hematopoietic Cells

荧光光度法二氢叶酸还原酶抑制剂筛选模型的建立和应用

Establishment and Its Application of Screening Model for Dihydrofolate Reductase Inhibitors by Fluorophotometry

大肠杆菌二氢叶酸还原酶的基因克隆、重组蛋白纯化及蛋白质分子间的交联

Cloning , Expression , Purification and Protein Cross-linking of Escherichia Coli Dihydrofolate Reductase

突变型大肠杆菌二氢叶酸还原酶基因的合成

Synthesis of Mutants of Escherichia coli Dihydrofolate Reductase Gene

双突变二氢叶酸还原酶基因对小鼠的化疗保护作用

Double-mutant dihydrofolate reductase gene transfection into bone marrow cells protects mice from chemotherapy

毛细管电泳法研究中草药有效成分对二氢叶酸还原酶的抑制作用

Study of dihydrofolate reductase inhibitors from effective components in chinese herbal medicine by capillary electrophoresis

突变二氢叶酸还原酶基因转导及乳癌耐药细胞株的建立

Mutant dihydrofolate reductase gene transduction and establishing the drug resistant cell strain of breast cancer

二氢叶酸还原酶抑制剂的高效毛细管电泳法筛选模型的建立与应用

Development and Application of Screening Method for Steroid Dihydrofolate Reductase Inhibitors by High Performance Capillary Electrophoresis

二氢叶酸还原酶和谷氨酰胺合成酶双基因筛选扩增系统对外源基因表达的影响

Dual gene amplification and selection system with dihydrofolate reductase and glutamine synthetase genes effectively increase the foreign gene expression

二氢叶酸还原酶抑制剂研究&2,4-二氨基-5-（取代苄氨基）嘧啶类的合成及其抑酶活性

Studies on dihydrofolate reductase inhibitor synthesis and enzyme inhibitory activity of some derivatives of 2,4-diamino-5 - substituted benzyl amino pyrimidines

叶酸受体及二氢叶酸还原酶在人乳腺癌细胞MCF-7/ADR多药耐药中的作用

The Role of Folate Receptor and Dihydrofolate Reductase in Multidrug Resistance of the Human Breast Cancer Cell Line MCF-7 / ADR

二氢叶酸还原酶缺陷型中华仓鼠卵巢细胞偏爱密码子的研究按大肠杆菌偏爱密码子化学合成人干扰素α-2b基因在大肠杆菌中高效表达的研究

Codon usage of Chinese hamster ovary cells Study on High Expression of the Chemical Synthetic Human Interferon α - 2b Gene in E.coli