Restriction enzyme assay , PCR and sequencing confirmed that the TK gene was obtained successfully . Only one Kpn ⅰ site and a transcription termination signal TTTTT N ( T ) T were found in the gene .
酶切鉴定、PCR鉴定和测序结果表明，成功获得了TK基因，获得的TK基因内存在一个KpnⅠ酶切位点和编码区的早期转录终止信号TTTTTN（T）T。
ttttt