subclone

The system capacities of the G. Isolation and identification of subclone cell lines with different metastatic capacities from human osteosarcoma

高低不同转移特性骨肉瘤亚克隆细胞系的分离与鉴定

Subclone , Expression , Purification and Its Structure Analysis of Heptad Repeat Regions from the F Protein of Newcastle Disease Virus

新城疫病毒F蛋白七肽重复序列区的亚克隆、表达、纯化及结构分析

Selection and subclone of the transforming genes of human papillomavirus type 16

人乳头瘤病毒16型转化基因的筛选及次级克隆

Construction of Subclone Library of Dragline and the Determination of Its Partial Sequence

拖丝蛋白全基因亚克隆文库的构建和部分序列的测定

Subclone strains isolation of human poorly differentiated nasopharyngeal carcinoma cell strain and observation of their growth characteristics

人低分化鼻咽癌细胞克隆亚系的分离及生长特性观察

The subclone was identified by restriction enzyme digestion .

亚克隆经酶切鉴定正确。

Construction of Subclone Library for Wheat Megabase BAC DNA

长片段小麦细菌人工染色体DNA亚克隆文库的构建

Significance of subclone and fully sequencing of gene encoding spider dragline silk protein for researching its structure and function

蜘蛛拖丝蛋白基因亚克隆与全序列测序对其结构与功能了解的意义

Study on subclone of naphthalene degrading gene of Pseudomonas aeruginosa pic-n

铜绿假单胞菌PIC－N萘降解基因的研究

Study on relationship between the infection of the different toxicity subclone of helicobacter pylori and idiopathic thrombocytopenic purpura

不同毒力亚株幽门螺杆菌感染在特发性血小板减少性紫癜发病中的意义

AIM : To subclone upstream stimulatory factor ( USF1 ) gene in mouse tooth germ .

目的：克隆牙胚中上游刺激因子（UpstreamStimulatoryFactor，USF）基因编码区特异片段。

Studies of spontaneous metastasis of transplantable subclone cells and the tissues of metastatic foci of nasopharyngeal carcinomas in nude mice

鼻咽癌克隆株及其转移灶组织裸鼠移植瘤自发性转移的研究

Objective To supply a good experimental model of studying osteosarcoma metastatic mechanism by establishment of human osteosarcoma MG-63 subclone cell lines with different metastatic capacities .

目的建立高低不同转移特性人成骨肉瘤MG63亚克隆细胞系，为研究成骨肉瘤转移机制提供较好的实验模型。

Subclone the positive cells by limiting dilution for several times to get identical cells , we got two positive hybridoma cells named D3 and F6 .

使用有限稀释法克隆筛选后得到了两株阳性杂交瘤细胞，并分别命名为D3和F6。

Finally one subclone in primary tumor acquires the full metastasis capacity and gives rise to a metastatic tumor . In this model , metastasis represents the end stage of evolution .

最后，原发肿瘤的一个亚克隆获得了完全的转移能力并形成转移灶，转移代表肿瘤演进的最后阶段。

Conclusion Construction of those clones will facilitate further to subclone the genes encoding S japonicum protective antigens into eukaryotic expressive plasmid and to determine their sequence .

结论为可能编码保护性抗原的血吸虫基因测序和将其亚克隆入真核表达质粒奠定基础。

Methods High invasive and metastatic subclone of bladder cancer cell line was screened in vitro and in vivo , tumor cell basement adhesive strength was measured using micropipette aspiration technique .

方法：利用体内和体外两种方法，分离出具有高浸润转移能力的细胞亚系，并用微管吸吮系统直接测定细胞与人工基底膜间的粘附力。

Conclusion : Repeated intravenous injection of lung cancer cells maybe a possible method to establish a highly brain metastatic subclone and an in vivo model of experimental brain metastasis in nude mice .

结论：应用肺癌细胞株行反复裸鼠体内接种，是建立肺癌脑转移实验动物模型的可行方法。

Oral vaccine candidate of Rabies virus strain SRV9 is an intermediate plaque subclone of SAD B19 and has been used in field with good safety and immunogenicity .

狂犬病病毒SRV9株是经克隆获得的中等蚀斑口服弱毒疫苗候选株，具有安全和免疫原性较好等优点。

Objective To subclone influenza virus RNA polymerase PB1 1 , construct the recombinant expression plasmid , express it and gain recombinant polypeptides for providing the basis of functional study on the polymerase .

目的将流感病毒RNA聚合酶PB11亚基进行亚克隆、表达，获得重组的亚克隆多肽，为进一步研究PB1亚基功能奠定基础。

Results The subclone bladder carcinoma cell lines , stably expressing bcl-2 and neo gene respectively , were successfully selected , named as BIU87 / bcl-2 and BIU87 / neo .

结果建立分别稳定表达bcl-2基因、新霉素抗性基因（neo）的膀胱癌亚克隆细胞株BIU87/bcl-2、BIU87/neo。

Although majority of the tumor come from monoclone , as a result of the tumor genetics instability and the target organ selective action , the performance of tumors variate unceasingly and some subclone obtains the metastatic phenotype .

虽然大多数肿瘤是单克隆起源的，但由于肿瘤的遗传学不稳定性和靶器官的选择作用，肿瘤常常表现为一个不断变异的群体，有些瘤细胞亚群得以杂在靶器官优势生长，获得转移表型。

The spleen cells of immunized mice were fused with SP2 / 0 myeloma cells . Indirect enzyme linked immunosorbent assay ( ELISA ) was used to screen hybridoma cells and limited dilution method was performed to subclone positive clones .

取免疫小鼠脾细胞与SP2/0细胞融合，采用间接ELISA法检测筛选阳性细胞孔，再经有限稀释法克隆培养，筛选稳定分泌单克隆抗体的细胞株。

Methods Extract total RNA from human liver tissue , amplify the encoding region of MBL by RT-PCR , clone into vector pGEM-T-easy , then subclone to plasmid pET-30 ( a ) for expression under induction of IPTG .

方法提取肝总RNA，RT-PCR扩增MBL编码区序列，并克隆到pGEM-T-easy载体上，再亚克隆到pET-30（a）上。

Purpose : To subclone the malignant transformants of human bronchial epithelial cell line ( BEP2D ) induced by α - particles and to analyse their karyotypes and capacity of rejoining DNA double-strand breaks ( DSB ) .

目的：建立α粒子诱发人支气管上皮细胞（BEP2D）恶性转化细胞的克隆细胞系，研究其核型和DNA链断裂修复能力。

Transfect CHO cells with the fusion gene and detect the expressed product . Results The primary expression level of the constructed chimeric antibody in CHO cells was 0.71 μ g / ml , and that after subclone was 0.95 μ g / ml.

结果构建的嵌合抗体基因在CHO细胞中的初始表达量为0.71μg/ml，亚克隆后表达量达0.95μg/ml。

The authors summarized the advances in the studies of resistance mechanism , identical tech - nique , fungus strain , screening for resistant sources , resistance heritance , selection of resistant subclone for sugarcane smut , and put forward some outlooks about these aspects .

综述了国内外在甘蔗抗黑穗病机制、抗病鉴定技术、病原菌生理小种，抗源筛选、抗性遗传以及抗病体细胞亚无性系筛选等方面的研究进展，并就上述诸方面提出一些展望。

Methods The full length human tissue factor pathway inhibitor ( TFPI ) gene was extracted by RT-PCR . The Kozak sequence and subclone sites were led in TFPI gene . Subsequently , the ( Kozak ) TFPI sequence was connected with T vector for sequencing .

方法用RT-PCR的方法提取全长人组织因子途径抑制因子（TFPI）基因，引入Kozak序列和亚克隆位点，将（Kozak）TFPI序列连入T载体中测序。

Objective To subclone human cDNA of gene of phosphatase and tensin homology deleted on chromosome ten ( PTEN ) and construct plasmids of tetracycline ( Tet ) responsive element , which regulates and controls the expression of PTEN ( pTRE PTEN ) .

目的亚克隆野生型与张力蛋白在10号染色体同源缺失的磷酸酶（PTEN）基因，构建四环素反应性元件调控的与张力蛋白在10号染色体同源缺失的磷酸酶反应质粒（pTREPTEN）。