poz

Cloning and Expression of Quail gcl BTB / POZ Domain Coding Sequence

鹌鹑gcl基因BTB/POZ结构域编码序列的克隆及组织中的表达

Objective To study the role of a BTB / POZ domain protein in the expression of hsp90 α gene .

目的探讨人BTB/POZ结构域GAGA元件结合相关蛋白（GRP）在hsp90α基因表达中的作用。

A gcl conserved sequence of 319 bp , which contained BTB / POZ domain coding sequence , was isolated from Quail ( Coturnix coturnix ) genome DNA using the BTB degenerated primers .

本研究应用简并PCR克隆技术，在鹌鹑（Coturnixcoturnix）中扩增得到319bp的gcl基因（包含BTB/POZ结构域编码序列）保守序列。

Germ cell-less ( GCL ) plays an essential role in the formation of primordial germ cells . An evolutionally conserved domain , BTB / POZ domain , was found in the GCL homologue proteins of Drosophila , zebrafish , medaka and mouse .

Germcell-less（GCL）是与原始生殖细胞发生相关的重要因子，果蝇、斑马鱼、青鳉鱼和小鼠的GCL蛋白都含有一个进化上保守的BTB/POZ结构域。