limiting dilution

Limiting dilution assay was performed as described previously ( 6,7 ) .

按照前述操作（6.7）进行有限稀释法检测。

The hybridoma cells capable of producing antibodies against recombinant SEI were screened by indirect ELISA and cloned by limiting dilution .

用间接ELISA和有限稀释法对可产生抗SEI抗体的杂交瘤细胞进行筛选和克隆扩增。

Methods Using modified limiting dilution assay .

方法：改良极限稀释定量PLR法。

Cell limiting dilution method is the easiest practice , least damage and higher cloning efficiency .

细胞稀释法操作简单易行，对细胞伤害小，克隆率较高。

Study on the Relationship Between Macrophage and Leishmania Major by Use of Limiting Dilution Assay

应用有限稀释测定法对巨噬细胞与利什曼原虫相互关系的研究

The surviving fraction of the clonogenic cells was also measured by limiting dilution method .

同时以极限稀释法检测其克隆源性细胞存活比率。

Methods Using modified limiting dilution assay . Methods T cell clones were isolated by limiting dilution .

方法通过有限稀释法建立T细胞克隆。

Cells which maintain their biological characteristics were effectively isolated by Enzymatic and purified by limiting dilution cloning .

结论：1.酶解组织块法有效分离到人牙周膜细胞群，有限稀释克隆纯化获得的牙周膜干细胞保持了其生物学特性。

The response of cells to anti-tumour drugs estimated by limiting dilution in microwell culture

极限稀释法微孔池培养测定细胞对抗癌药物的剂量反应曲线

Cells purified by limiting dilution cloning appeared Spindle . The results show that the cell is a slow proliferation cycle stem cells by MTT detection . 2 .

结果：1.有限稀释克隆纯化后的细胞呈长梭形，通过MTT检测，证明该细胞是慢增殖周期的细胞。

Subclone the positive cells by limiting dilution for several times to get identical cells , we got two positive hybridoma cells named D3 and F6 .

使用有限稀释法克隆筛选后得到了两株阳性杂交瘤细胞，并分别命名为D3和F6。

Quantitative analysis of IgH and V δ 2D δ 3 gene rearrangement and its clinical significance in children with acute lymphoblastic leukemia by limiting dilution assay Diluted Chinese Style

极限稀释法对急性淋巴细胞白血病IgH和Vδ2Dδ3基因重排的定量检测及其意义

TNF gene was transfected into murine LAK cells by retrovirus . Three groups of LAK cells were cloned by G418 resistant selection and limiting dilution techniques .

在逆转录病毒介导下将TNF基因转染入小鼠LAK细胞中，采用G418抗性筛选和有限稀释法，将LAK细胞克隆化，并筛选出3株分泌不同水平TNF的LAK细胞克隆。

A method of limiting dilution assay was used for cloning the hybridoma cells with positive reaction . Screeninged the positive reaction hybridoma cell with ELISA experiment after cloning every time .

采用有限稀释法对阳性杂交瘤细胞进行克隆，每次克隆后均用ELISA法进行阳性克隆筛选。

Methods : T cell clones ( TCC ) were derived from nose biopsy specimens ( NBS ), bronchoalveolar lavage ( BAL ) and peripheral blood ( PB ) under limiting dilution condition .

方法：用有限稀释法从WG患者鼻粘膜活检标本（NBS）、支气管肺泡灌洗液（BAL）及外周血中克隆出T细胞。

The positive rate of antibody is 60 % by the quick ELISA , The positive clones were chosen and cloned by limiting dilution for 3-5 times , Finally seven clones were established , secreting SPA-specific antibodies .

以快速ELISA法检测抗体，阳性率60%。5次有限稀释法克隆化后获得7株杂交瘤细胞，作中和抑制试验，证实为SPA特异性抗体。

Method Freshly isolated mononuclear cells in the liver and peripheral blood of CHB patients were obtained , and HBcAg-specific T helper cell clones were subsequently established by limiting dilution , and the analysis of these clones was conducted .

方法采用有限稀释法，建立CHB患者外周血及肝组织HBcAg特异性Th细胞克隆，并对所获得细胞克隆进行分析。

[ Methods ] BALB / c mice were intraperitoneally immunized with purified Botulinum neurotoxin type B ; the splenocytes from immunized mice were fused with myeloma cells Sp2 / 0.Hybridoma cells were screened by indirect ELISA and limiting dilution method .

[方法]采用纯化的B型肉毒毒素免疫BALB/c小鼠，用免疫小鼠的脾细胞与骨髓瘤细胞Sp2/0融合，筛选阳性克隆。

In the past , chromium-51 release assay and limiting dilution analysis assay are usually used to detect antigen-specific T cells . But the two methods are time consuming , taking a lot of work , insensitive and cannot study antigen epitope specific T cells at single cell level .

在过去，通常用铬51释放测定法和有限稀释分析法来测定抗原特异T细胞反应，但这两种方法耗时、费工、不敏感，而且不能在单个细胞水平上研究抗原特异性CTL。