intein

Some recent advance in the study of the structure and function of the intein as well as the mechanism of intein-mediated protein splicing and its application are described in this review .

介绍了蛋白质内含子的结构、功能和其介导的蛋白质剪接机理及应用研究进展。

An intein can split into two pieces as N-fragment and C-fragment of intein ( IN and Ic ), and express by two different open reading frame .

断裂蛋白质内含子是指蛋白质内含子被分裂成两部分即N端区域（IN）和C端区域（IC），分别由不同的开放阅读框表达。

On the Constitutive Relations of Concrete Intein and Protein - splicing

蛋白内含子与蛋白剪接

However , no evidence is available for intein removal in vivo in cyanobacteria .

但是，没有证据表明在蓝细菌细胞体内蛋白内含子（内含肽）的切除。

There are variety of chemical methods and the use of intein ' trans-splicing for protein labeling .

目前对蛋白质进行标记的方法主要有各种化学方法和利用蛋白质内含子的反式剪接进行的标记。

Several intein elements are also present in cyanobacteria , and some of them have been studied biochemically in vitro .

在蓝细菌中存在着许多的蛋白内含子（内含肽），其中，许多已经在体外对其进行了生物化学的研究。

Combined with the structure information , the analysis of intein mutants can provide large amount of information about the relationship between the intein structure and function .

分析高剪接活性突变体的结构信息，为研究蛋白质内含子结构和功能之间的关系带来更多的信息和新的启示。

Protein splicing , which is an intein mediated posttranslational processing , involves a series of intramolecular cleavage - ligation reactions .

蛋白质剪接是蛋白质内含肽介导的，一种在蛋白质水平上翻译后的加工过程，它由一系列分子内的剪切-连接反应组成。

Based on its splicing and cleaving functions , intein has many potential applications in protein engineering , such as for protein purification , as a molecular switch , in biosensors , or for protein labeling .

基于蛋白质内含子的剪接反应和突变后的断裂反应，蛋白质内含子在蛋白质工程中有着广泛的应用，例如在蛋白质纯化、分子开关、生物传感器、蛋白质标记等领域。

Intein able to catalyze self-splicing from the precursor protein and the exteins were ligated with a peptide bond . This post-translation process was termed protein splicing .

在宿主蛋白质的成熟过程中，蛋白质内含子可以催化自身从蛋白质前体中剪切下来，同时将两侧的外显子以肽键连接，这种蛋白质翻译后的成熟过程称为蛋白质剪接（proteinsplicing）。