METHODS : By using human Tac antigen signal peptide and κ chain and γ 1 heavy chain gene fragments of human Ig , we constructed a versatile expression vector for human mouse chimeric antibody and used it to transfect cultured 293T cells .
方法：用人Tac抗原信号肽以及人IgCκ基因和γ1CH基因，构建人鼠嵌合抗体的表达载体，并转染真核细胞293T进行表达。
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