dNTP

Study About the Relationship of dNTP , Mg ~ ( 2 + ) and Multiplex Polymerase Chain Reaction

在多基因PCR中对dNTP与Mg~（2+）的浓度关系的研究

One mechanism identified for inhibition of DNA repair or replication by p21 is depletion of dNTP pools .

P21介导DNA修复和复制的抑制机制之一是通过耗竭dNTP库。

After testing on the annealing temperature and the concentration of primer , dNTP and MgCl_2 , a suitable PCR system was established .

在对引物、dNTP、MgCl2的浓度及退火温度等参数进行测试后，建立了合适的PCR反应体系。

Screening ISSR-PCR the template concentration , primer concentration , dNTP concentration and Taq enzyme dosage , optimizing ISSR-PCR system . 4 .

分别筛选ISSR-PCR扩增的模板浓度、Taq酶用量、引物浓度及dNTP浓度，优化ISSR-PCR扩增体系。

Objective : To investigate and compare the effects of deoxynucleoside triphosphates ( dNTP ) on human lymphocytes and plant root tip cells SCE .

目的：研究和比较4种三磷酸脱氧核苷（dNTP）对人外周血淋巴细胞和植物根尖细胞姐妹染色单体交换（SCE）的影响，探讨其发生的机理。

Moreover , the concentrations of Mg2 + , dNTP and Taq DNA polymerase in allele-specific PCR were higher than that in conventional PCR .

在等位基因特异PCR中，Mg2+、dNTP及taqDNA聚合酶的用量均大于普通PCR。

It also showed that the PCR reaction residues ( including dNTP , primers , and metal ion ) affected badly on the sequencing quality , so the purification of PCR products was necessary before sequencing .

PCR反应体系残留混合物（dNTP、引物和盐离子等）对其测序质量有明显不利影响，PCR产物纯化后其测序质量能明显提高；

The procedure of DDRT-PCR applicable for silver staining was optimized by adjusting the amount of several critical reagents , including total RNA , anchor primer , arbitrary primer , cDNA and dNTP .

通过调整DDRT-PCR中总RNA、锚定引物、随机引物、cDNA和dNTP等关键试剂的用量，优化了适用于银染检测的DDRT-PCR方法。

Strand-displacement amplification is a sensitive , rapid and specific isothermal DNA amplification method . But presence of dNTP [ α S ] in the reaction limits the application of SDA in molecular biology .

链置换扩增技术是一种快速、灵敏、特异的DNA等温扩增方法，但硫修饰的dNTP的掺入限制了其在分子生物学领域的应用范围。

In the absence of target DNA , complementary DNA showed the status of the single-stranded , RCA reaction was initiated by addition of the circular template , polymerase , ligase and dNTP .

在没有目标物DNA时，互补DNA呈现单链状态，其在环状模板，聚合酶，连接酶以及dNTP的存在下进行RCA扩增。

A amplified fragment length polymorphism ( AFLP ) molecular marker system on B. tabaci was funded by adjusting the DNA density , Mg ~ 2 + density , dosage of dNTP and other reactions parameter etc.

通过DNA模板浓度、Mg~（2+）浓度、dNTP用量以及其它反应参数等的摸索、调整，建立了一套完善的烟粉虱AFLP分子标记技术体系。

With orthogonal experiment , this paper studied the effects of the concentrations of Mg ~ ( 2 + ), dNTP and primer , the anneal temperature , and the extending time and cycling times on RAPD of soil microbes .

采用正交实验设计，对影响土壤微生物RAPD扩增体系的Mg2+、dNTP浓度及引物浓度进行了研究，同时对退火温度、延伸时间及循环次数进行摸索。

[ METHOD ] Different levels on concentration of DNA template , primer , dNTP mixture and Taq DNA polymerase and annealing temperature were set in this experimentation . All factor influence for SRAP-PCR in peanut genome was investigated .

【方法】对模板DNA、引物、dNTPmixture、taqDNA聚合酶浓度及退火温度设置不同梯度，研究各因素对PCR结果的影响；

Concentration of primer , magnesium chloride , dNTP , template DNA , Taq DNA polymerase and annealing temperature in PCR and the quantity of PCR product and endonuclease and digesting time in digestion process affect the profiles of the whole experiment .

PCR反应体系中不同模板含量、引物浓度、taqDNA聚合酶用量、dNTP浓度、Mg2+浓度、退火温度和酶切体系中PCR产物量、内切酶量、酶切时间等对反应结果均有不同程度的影响。

Third , assure quality and amount of mRNA , reduce amount of dNTP , select of suitable temperature of inverse transcription and PCR renaturation ( the primers to anneal to the template DNA strands ), the methods can optimize parameter of PCR .

3保证起始（模板）mRNA质量和浓度，降低dNTP浓度，选择最适的反转录和PCR退火温度，可优化PCR参数。

The results indicated that the concentration of primers , the annealing temperature and the number of cycles have more effect on the efficiency of multi-PCR , but the concentration of dNTP , the concentration of Mg ~ ( 2 + ) and the content of Taq have fewer .

试验结果表明，引物浓度、退火温度和循环次数对该反应体系影响较大，而dNTP浓度、Taq酶含量以及Mg2+浓度的影响较小。