cccDNA

CccDNA plays a role in the liver disease development

共价闭合环状DNA在肝病发展中的作用

Many years study show that cccDNA plays an important role in every process of the liver disease development .

多年研究发现，cccDNA在肝病发展的每个过程中起着重要的作用。

Establishment and Application of a Fluorescent Quantitative PCR Method for cccDNA of HBV

HBVcccDNA荧光定量PCR检测方法的建立及初步应用

The Clinical Study of Detecting cccDNA in Patients ' Sera Suffered Chronic Hepatitis B

慢性乙型肝炎患者检测血清cccDNA的临床意义

Relationship of detection of cccDNA of hepatitis B virus and serum and pathology

乙型肝炎病毒cccDNA检测与血清学及病理诊断关系

Detection of HBV cccDNA in chronic hepatitis B patients

慢性乙型肝炎患者HBVcccDNA的检测

The positive rate of HBV cccDNA was higher when virus replication was active .

本方法临床标本初步检测结果显示病毒复制活跃患者肝组织中HBVcccDNA的阳性率较复制低的患者为高。

Objective To establish a method for detecting HBV cccDNA in hepatocytes of chronic hepatitis B patients .

目的建立一种肝活检组织中HBV共价闭合环状DNA的定量检测方法。

Detection of HBV antigens and cccDNA in glomeruli of patients with HBV infection and renal disease

乙型肝炎病毒感染肾炎患者肾组织病毒抗原和复制中间体的检测及其意义

Effects of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation

金丝桃苷对鸭乙肝病毒cccDNA清除及免疫调节作用探讨

Because HBsAg and cccDNA are biologically relevant , the extracellular HBsAg level was also measured quantitatively in the present research .

因为HBsAg生物学意义和cccDNA相近，本课题对HBsAg胞外分泌水平进行了测量。

Advance in HBV cccDNA detection

乙型肝炎病毒cccDNA的临床检测研究进展

Covalently closed circular DNA ( cccDNA ), the primary template for viral replication , is a key factor for persistent infection .

HBV共价闭合环状DNA（covalentlyclosedcircularDNA，cccDNA）作为HBV基因组的原始复制模板，是HBV持续感染的关键因素。

CccDNA was all negative .

各时相cccDNA检测始终阴性。

[ Objective ] To study the clinical significance of serum hepatitis B virus covalently closed circular DNA ( HBV cccDNA ) determination in patients with chronic HBV infection .

探讨慢性乙型肝炎病毒感染者外周血乙型肝炎病毒共价闭合环状DNA检测的临床意义。

Objective To study the effect of hepatitis B virus ( HBV ) C protein C-terminal small interference RNA ( siRNA ) on reduction of HBV covalently closed circular DNA ( cccDNA ) level .

目的研究乙型肝炎病毒（HBV）C蛋白羧基端短小的干扰RNA（siRNA）对HBV超螺旋双链闭环DNA（cccDNA）水平的影响。

[ Methods ] Serum HBV cccDNA levels were determined in 325 patients with chronic HBV infection using molecular-beacon Polymerase Chain Reaction technique . The relationship between serum HBV cccDNA , serum total HBV DNA and clinical parameters were analysed .

采用PCR荧光分子信标技术对325例慢性乙型肝炎病毒感染者外周血HBVcccDNA进行检测，分析HBVcccDNA水平与病毒复制指标及病情轻重的关系。

HBsAg and HBeAg in the supernatant were detected by ELISA , HBcAg in nuclei by immunohistochemistry , HBV DNA in supernatant and cells by quantitative fluorescence PCR , and cccDNA in cultured cells by nest PCR .

应用ELISA法检测培养上清中的HBsAg和HBeAg，免疫组化法检测细胞核中的HBcAg，荧光定量PCR法检测上清和细胞中HBVdna，巢式PCR法检测细胞中cccDNA。

The pBR322 recombinant plasmids DNA products , which purified by phenol-extract and equilibrium centrifugation in CsCl - ethidium bromide gradients , have been shown as mainly spatial conformation of covalently closed circular DNA ( i. e. cccDNA ) by agarose gel electrophoresis for homogeneity detection .

将pBR322重组质粒DNA纯化，经琼脂糖凝胶电泳鉴定其主要具共价闭合环状空间构象。