VIGS

VIGS : the Revolution of Plant Function Genomics Research

VIGS：植物功能基因组学研究的革命

We have successfully established the VIGS system in cotton and its application in cotton resistance to Verticillium wilt .

本研究系统开展了棉花病毒诱导的基因沉默体系的建立及其在棉花抗黄萎病中的应用。

Virus-induced gene silencing ( VIGS ) is an efficient tool to study the functions of plant gene .

病毒诱导的基因沉默（Virusinducedgenesilencing，VIGS）是一快速高效的研究植物基因功能工具。

These 19 genes were selected as targets for VIGS analysis in N.benthamiana .

选取这19个本氏烟或烟草同源基因作为沉默的靶标基因，对其在抗病性中的作用进行了检测分析。

The review provides the prospects for VIGS as a powerful and high-through tool for assessing and characterizing the function in plant function genomics .

同时，展望了VIGS技术作为植物功能基因组学功能分析的高通量技术平台的美好前景。

Virus-induced gene silencing ( VIGS ) of papaya .

番木瓜中病毒介导的基因沉默（VIGS）实验。

The main results were as follows : ( 1 ) Role of the ubiquitin fusion degradation protein ( 18-8 ) gene in regulation of plant disease and stress resistance was analyzed by VIGS technique .

获得了以下结果：（1）采用VIGS技术分析了泛素融合降解蛋白（ubiquitinfusionprotein，18-8）基因对植物抗病和抗逆性的调控功能。

How DNA and RNA viruses have silence plant gene expression were examined and the advantages and disadvantages of VIGS in determining gene function and the future trend of virus induced gene silencing were discussed .

阐述了DNA和RNA病毒诱导植物基因沉默的机理，同时讨论了利用病毒诱导的基因沉默（VIGS）鉴定植物基因功能的优缺点和将来的发展趋势。

In summary , VIGS technique was used to identify 38 potato horizontal resistance-related genes ' function in N. benthamiana . Totally , 12 potentially important genes in potato horizontal resistant to late blight were identified .

综上，本研究利用VIGS技术高通量在烟草中鉴定了38个马铃薯水平抗性相关基因功能，筛选出12个可能在马铃薯晚疫病水平抗性中具有重要作用的基因。

The method of virus-induced gene silencing ( VIGS ) was used to silence target genes in the resistant line . Quantitative PCR and enzyme activity assay demonstrated that the expression of target genes decreased to different degrees in gene-silenced plants .

利用病毒诱导的基因沉默技术在抗病材料上创造了目标基因的沉默，定量PCR和酶活性测定均证明目标基因表达量都有不同程度的明显下降。

This research focuses on cloning of AS genes from tomato and rice , and analyzing the its function in regulation of disease resistance employing virus-induced gene silencing ( VIGS ) and transgenic ( overexpressing and RNAi ) technique .

本研究拟分别克隆番茄和水稻AS基因，并采用病毒诱导的基因沉默（Virusinducedgenesilencing，VIGS）技术和转基因超量表达和RNAi技术，研究AS基因对植物抗病性的调控功能。

Although VIGS can be performed in tomato fruit by all of the means mentioned above , the most effective method is to inject the TRV-vector into the carpopodium of young fruit attached to the plant about 10 days after pollination .

尽管上述四种方法均能对番茄果实形成有效侵染，但花后10天的番茄果实果柄注射是在番茄果实中建立病毒诱导基因沉默的最有效方法。

By contrast , when P ⅰ ⅱ gene of JA signalling pathway was inhibited by VIGS , there was a significant increase in nematode infecting tomato root and an obvious inhibition in the resistant effect of exogenous JA , SA and NO on root-knot nematode .

而VIGS抑制JA信号路径PIⅡ基因表达后，显著提高了番茄根系中线虫的侵染程度，且明显抑制了外源JA、SA及NO对根结线虫的抗性效应。