Protopectinase

Study on Strains Screening for Producing Protopectinase and Its Fermentation Properties

微生物原果胶酶高产菌株的筛选及发酵特性的研究

The most suitable conditions for pectin production by protopectinase were achieved as follows .

研究了不同条件对原果胶酶生产果胶的影响。

Ca2 + and Tween-20 were able to enhance the production of protopectinase .

钙离子及Tween-20均能促进该酶的产生。

Purification and characteristics of protopectinase ;

酶的分离纯化与酶学性质的研究；

The enzyme that catalyzes water-soluble pectin substance from protopectin is protopectinase .

原果胶酶是能水解不溶性果胶物质为可溶性果胶物质的一类酶。

The main results were as follows : ( 1 ) A strain XZ-131 highly producing protopectinase was screened out of 137 strains .

对从不同样品中分离得到的137株菌株进行摇瓶试验，得到一株产原果胶酶活性较高的菌株，将其命名为XZ-131。

Extration of Pectin by protopectinase could be used to reduce the shortcomings in current industry processing of pectin production as well as to stabilize the pectin with high quality .

利用微生物原果胶酶酶法提取果胶，可克服工业酸法生产的诸多不足并得到质量稳定的果胶产品。

So far , researches on microbial protopectinases and applications of those have been not reported in China , and protopectinase expressed in Pichia pastoris have been not reported in the world .

在中国，有关微生物原果胶酶的研究和应用技术还未见报道，原果胶酶基因在Pichiapastoris中表达的研究在国内外未见报道。