MLCK

Purification of MLCK in bovine cardiac muscles and studies of its some biochemical characters

牛心肌肌球蛋白轻链激酶的提纯及其一些生物化学性质的研究

Application of polymerase chain reaction in construction of MLCK expression vector

聚合酶链反应在MLCK表达载体构建中的应用

The expression of MLCK was analyzed in renal tissue by immunohistochemistry and western-blot methods respectively .

通过免疫组化与Western-blot检测肾组织肌球蛋白轻链激酶表达。

Conclusion The expression increase of MLCK in bronchial smooth muscle may be correlated with asthmatic pathogenesis .

结论支气管平滑肌MLCK表达增加可能与哮喘发病有关。

The myosin light chain kinase ( MLCK ) is important in regulating the smooth muscle contraction .

肌球蛋白轻链激酶（MLCK）在调节平滑肌细胞收缩过程中具有十分重要的作用。

SL extracts inhibiting the cytoskeleton rearrangement function mainly related with Ca + dependent MLCK pathway .

SL提取物抑制细胞骨架重排的作用主要与Ca2+依赖的MLCK通路有关。

AIM To study the effect of Vit E on the MLCK activity and expression in the liver of atherosclerosis model rabbits .

目的探讨维生素E（VitE）对动脉粥样硬化模型兔肝脏MLCK活性及表达的影响。

The Characterization of MLCK Mediated Ca ~( 2 + ) - independent Phosphorylation of Myosin Light Chains

肌球蛋白轻链的非Ca～（2+）依赖性磷酸化特征及其影响因素

MLT decreased the phosphorylation of MAPK which regulate the expression and activity of MLCK and endothelial permeability .

而MLT通过降低相关蛋白的磷酸化调控MLCK的活性和表达以及动脉内膜通透性。

In addition , the expression levels of MLCK and p-MLC were significantly reduced in the kidney of diabetic rats compared with control group .

另外，糖尿病组大鼠肾脏中MLCK和p-MLC表达水平明显低于正常组大鼠，而总的MLC的蛋白水平无明显变化。

The intestinal barrier dysfunction induced by hypoxia is also accompanied by the increase of both MLCK protein expression and MLC phosphorylation .

缺氧引起肠上皮细胞屏障功能损害，并伴随有MLCK蛋白表达及MLC磷酸化增加。

The purpose is to explore the non-kinase activity of MLCK and to elucidate the molecular mechanism of its activity in regulating contraction .

为探寻MLCK的非激酶活性区域对MLCK活性的影响，以进一步阐明MLCK的非激酶活性在调节平滑肌收缩过程中的分子机制。

Myosin light chain kinase ( MLCK ) possesses kinase and non-kinase properties , and both properties play important roles in the regulation of smooth muscle contraction .

肌球蛋白轻链激酶（MyosinLightChainKinase，MLCK）具有激酶活性和非激酶活性，在平滑肌收缩过程中起着关键酶调控的作用。

Research confirms that hemodynamic shear stresses acting on osteoblast contraction have two ways which are Ca2 + dependent ( MLCK pathway ) and non Ca2 + dependent .

研究证实，血流切应力对成骨细胞的收缩具有Ca2+依赖性（MLCK通路）和非Ca2+依赖性（ROCK通路）两种途径。

Integrin and MLCK are two key proteins involved in the process of tumor cell invasion and metastasis . The former regulates cell adhesion and the latter drives the cells to migrate .

整合素和MLCK是肿瘤细胞侵袭转移过程中调节细胞粘附稳定性和为细胞迁移提供驱动力的两个重要分子。

Gastrointestinal tissue contents of myosin light chain kinase : The content of MLCK in colon smooth muscle in blank group is highest , that in model group decreased significantly ( compared to blank group P0.05 ) .

胃肠组织肌球蛋白轻链激酶的的含量：结肠平滑肌MLCK含量空白组最高，模型组明显下降（与空白组比较P0.05），而针刺组较模型组有所上升（P0.05）。

Objective : To establish the bronchial asthma model in rat ; To investigate the expression of myosin light chain kinase ( MLCK ) in bronchial smooth muscle of asthma rat model and explore the role of MLCK in asthmatic pathogenesis ;

目的：复制支气管哮喘（简称：哮喘）大鼠模型，研究其支气管平滑肌肌球蛋白轻链激酶（MyosinLightChainKinase，MLCK）在哮喘发生发展过程中表达的变化规律；

Such an effect could be significantly inhibited by a 30-min pretreatment of the cells with MLCK-specific inhibitor ML-7 . Inhibition of MLCK also reversed actin reorganization in the endothelial cells pretreated with the burn serum .

可以通过使用肌球蛋白轻链激酶特异性抑制剂ML-7预处理细胞30min来抑制这种效应。此外，ML-7还能够改善烧伤大鼠血清引起的内皮细胞内肌动蛋白重排。

The aim of this study is to investigate the mechanism of MLCK in diabetic nephropathy . Methods : Diabetes was induced by a caudal vein injection of streptozotocin . The diabetic rats were then randomly divided into diabetic group and insulin group .

本研究旨在探讨MLCK在糖尿病肾病中的作用机制。方法：尾静脉注射链脲佐菌素（STZ）制作糖尿病大鼠模型，糖尿病大鼠再进一步随机分为糖尿病组和胰岛素组。

It is generally accepted that the interaction between actin and phosphorylated myosin by myosin light chain kinase ( MLCK ) in a Ca2 + - CaM dependent manner is the principal mechanism of regulation of smooth muscle contraction .

目前已公认的平滑肌收缩与舒张的主要调节机制是在Ca2+的参与下，由肌球蛋白轻链激酶（MLCK）催化的磷酸化肌球蛋白轻链（MLC20）与肌动蛋白的相互作用而产生收缩。

In this study we isolated myosin light chain ( MLC ) and myosin light chain kinase ( MLCK ) from acute myocardial injury model of dog caused by endotoxin shock . The kinetic change of MLCK during endotoxin shock was analysed .

以内毒素休克狗作为急性心肌损害的动物模型，从心肌中提纯肌球蛋白轻链（MLC）和肌球蛋白轻链激酶（MLCK），根据酶动力学分析法研究急性心肌损害对MLCK活性的影响。

Objectives To investigate the time-dependent effects of serum from burned rats on cytoskeletal filamentous actin ( F-actin ) reorganization by visualizing their distribution in human umbilical vein endothelial cell line ECV-304 and evaluate the role of myosin light-chain kinase ( MLCK ) in this process .

目的观察烧伤大鼠血清刺激人脐静脉内皮细胞株ECV-304引起的纤维状肌动蛋白在细胞内分布变化的时间效应以及肌球蛋白轻链激酶在其中的作用。