The mutant strains were identified by naringin screening plate.31 mutant strains which colonies grew well and transparent zone diameter / colony diameter larger than the original strain M53 ( 1.21 ) were picked .
挑取菌落生长旺盛、透明圈大的菌株,其中透明圈直径/菌落直径大于出发菌株M53(1.21)的突变菌株有31株,并对其进行摇瓶发酵复筛,测定酶活力。