DTNB

DTNB reaction was used to measure reduced glutathione ( GSH ) level .

DTNB反应测量还原型谷胱甘肽的水平；

Studies on the irreversible inhibition of creatine kinase from human muscle by DTNB

DTNB对人肌肌酸激酶不可逆抑制作用的研究

Methods GSH - Px livingness is determined by DTNB direct way in blood and tissue .

方法DTNB直接法测定血和组织中GSH-Px活力。

The effect of phospholipase A_2 inhibitor DTNB on the rat ischemia reperfusion arrhythmia

磷酯酶A2抑制剂DTNB对大鼠心肌缺血再灌注心律失常的影响

The micro DTNB colorimetric assay for human liver butyrylcholinesterase

人肝假性胆碱酯酶活性微量DTNB测定法

The irreversible inhibition of creatine kinase from human muscle during modification with DTNB has been studied .

本文研究了DTNB对人肌肌酸激酶的不可逆抑制作用。

2.3 GSH content : DTNB direct chromatometry .

3GSH含量测定：DTNB直接比色法。

Modification of protein thiol with NEM or DTNB after reduction with DTT leads to further decrease of specific binding .

DTT还原膜蛋白二硫键后再用NEM，DTNB修饰巯基，胰岛素结合活性进一步下降。

Reduction with DTT and treatment with NEM or DTNB result in changes in the binding activity of insulin receptor in partially purified human placenta membrane .

部分纯化的人胎盘膜经DTT还原，NEM，DTNB修饰蛋白巯基后，改变了胰岛素受体的结合活性。

The DTNB method , on the other hand , was quite robust and easy to operate , making it a promising alternative for regular determination of sulfite in foods .

DTNB法，另一方面，相当稳健和易于操作，这使它成为一种有前途的食品亚硫酸盐检测标准方法的备选方法。

The ipsilateral epididymides were collected for detecting the content of carnitine by DTNB technique and the apoptosis of epididymal epithelium by TUNEL technique .

TUNEL法检测附睾上皮细胞凋亡，DTNB法检测扭转侧附睾肉碱的含量。

After K ATP was blocked by glibenclamide , DTNB led to lethal ventricular fibrillation and increase LDH , higher than without DTNB ( P < 0.05 ) .

与glibenclamide组比，在glibenclamide阻断KATP后，DTNB可引起致死性心律失常，并可升高LDH值（P<005）；

Methods : 31 chronic renal failure patients and 19 healthy subjects were selected in this investigation to study the changes of acetylcholinesterase activity . The activity of acetylcholinesterase in serum was determined with DTNB method .

方法：用微量DTNB法测定31例尿毒症患者及19例健康对照者的血浆胆碱酯酶活力，判断其与肾功能损伤的相关性。

The contents of MDA and GSH were determined by TBA and DTNB methods , respectively . The gastric mucosal blood flow ( GMBF ) was measured with hydrogen gas cleanrance method .

MDA和GSH含量测定分别应用TBA和DTNB法，胃粘膜局部血流量（GMBF）测定应用氢气清除法。

Methods : NO output , iNOS ( inducible nitric oxide synthase ) activity and reduced glutathione ( GSH ) level were determined by Griess reaction , fluorimetry and DTNB colorimetry respectively .

方法：采用Griess反应、荧光法和DTNB比色法分别测定不同剂量的PPS作用小鼠腹腔巨噬细胞的NO生成量、诱导型一氧化氮合酶（iNOS）活性及细胞内还原性谷胱甘肽（GSH）含量的变化。

Methods The cordyceps was given 14 days , the SOD activity in red cells and GSH Px activity in blood and the LPO contents were determinated respectively by NBA , DTNB , TBA test methods .

方法经口给药14d后，用NBA法测定红细胞中的SOD活性；DTNB法测定全血中GSH-Px活性；

RESULTS : DTNB ( 16 mg / kg ) reduced the scores of arrhythmia , creatine kinase ( CPK ) and lactate dehydrogenase ( LDH ) ( P < 0.05 ) .

结果：与对照组相比，DTNB可降低心律失常评分、磷酸肌酸激酶（CPK）和乳酸脱氢酶（LDH）值（P<005）；

The activity of glutathione peroxidase ( GSH-px ) of hippocampus was determined by the method of DTNB . The mitochondrial membrane protein-bound Tb 3 + fluorescent intensity was measured by fluorospectrophotometry .

DTNB法测定谷胱甘肽过氧化物酶（GSHpx）活性；荧光分光光度法测定脑组织线粒体膜蛋白结合铽；

Methods The activity of acetylcholine esterase ( AChE ) and the density and affinity of M receptors in the cerebral cortex , hippocampus and striatum were measured with DTNB method and receptor radioligand binding method .

方法以DTNB法和受体放射性配基结合法检测大鼠皮层、海马及纹状体AChE活性和M受体密度及亲和力。

DTNB method was used in these experiments to investigate the effect of soman intoxication under high altitude hypoxia on blood or brain acetyl choline esterase ( AChE ) activities compared with that at plain in dogs and rats .

采用DTNB显色法，研究平原和模拟4000m高原缺氧条件下梭曼对犬、大鼠组织及全血AChE活力的影响。

The principle is that the concentration of total glutathione is proportional to TNB-forming rate resulting from the reaction between GSH and DTNB provided that NADPH and GR are used to keep the amount of total glutathione constant .

原理是，在NADPH和GR维持谷胱甘肽总量不变的条件下，GSH和D7NB反应，生成TNB的速率与样品中总谷胱甘肽量呈正比。

The serum liperoxide level , whole blood Cu · Zn superoxide dismutase content and glutathione peroxidase activity were determined in 45 cases of patients with acute leukemia and 39 cases of donors by TBA , RIA and DTNB direct method .

采用硫代巴比妥酸（TBA）法、放射免疫法和DTNB直接法对45例急性白血病（AL）病人和39例健康人血清LPO水平、全血Cu·Zn－SOD含量和GSH－Px活性进行了检测。

The ST of op and carbamate insecticides was significantly correlated with the sensitivity of the target enzyme ( AChE ) to the sulfhydryl reagent ( DTNB ) . It appeared that the ST was probably related to the sulfhydryl binding site of AChE .

之间，也只有6.86，有机磷和氨基甲酸酯杀虫剂对不同蚜虫的选择毒性与乙酰胆碱酯酶（AChE）对巯基试剂（DTNB）的敏感度有明显的相关性，说明其选择毒性与AChE的巯基结合部位有关。

When the enzyme was inhibited by AMP there were two SH groups on the enzyme which reacted rapidly with DTNB . The total detectable SH groups of the enzyme under AMP inhibition were 4 . Both the partially and completely modified enzymes showed three-fold increase in their activities .

受AMP抑制的酶2个快速反应巯基被DTNB修饰后，其pH7.5活力增加到对照酶活力的2.5倍，在这种条件下可反应的总巯基数为4，这时酶仍可保持在高活性状态。

Methods : GCF was collected from 44 sites of 23 patients with adult periodontitis . The volume of GCF was measured with Periotron 8000 . GCF GPx activity was determined by DTNB methods before and after treatment , and the periodontal parameters GI , PD and AL were recorded .

方法：选择23例成人牙周炎（AP）患者的44颗患牙，记录临床指标GI、PD、AL，并测定治疗前后GCFGPx水平。

However , for the acetylcholinesterase from snake venom ( Naja naja atra ), DTNB , TNB and TNB-Tch strongly activated it , with the exception of very lowconcentrations ( < 10 μ M ) of added DTNB and TNB , which caused slight inhibition .

对蛇毒乙酰胆碱酯酶，除了极低浓度（<10μM）DTNB和TNB有微弱的抑制作用外，TNB-Tch以及较高浓度的DTNB和TNB都具有强烈的激活作用。