DNJ

Extraction and Application Study of DNJ from Mulberry

桑树组织中生物碱DNJ的提取及应用研究

DNJ contents of leaf grow in upper was higher than middle and down leafs .

相同桑树品种，生长在上部的叶片DNJ含量高于中下部叶片。

Compared with DNJ , the inhibition effect in NAGase activity of quercetin is limited .

虽同为糖苷酶抑制剂但相比于DNJ，槲皮素对NAGase活性的抑制能力有限。

There was a close relationship between the effect of MLE in reducing blood glucose and the content of DNJ .

MLE的降血糖效果与DNJ的含量有密切关系。

Research progress of DNJ in mulberry twig

桑枝中1-脱氧野尻霉素（DNJ）的研究进展

In the progress of DNJ accumulative , samples picked in July to August were higher than others .

在DNJ积累的时间进程中，采收期的7-8月份含量高于其它时期。

Factors effecting on DNJ contents in the larva of the silkworm , Bombyx mori

家蚕幼虫体中1-脱氧野尻霉素含量的影响因素

So DNJ is an important physiological active material in mulberry leaves and silkworm body , especially when related in its reducing blood glucose .

因此DNJ是桑和蚕体中的重要生理活性物质，尤其是与其降血糖作用密切相关。

This test selects main active substances DNJ and quercetin from mulberry , mixed made into different concentration of solution , water treatment as controls .

本文以桑树中的主要活性物质DNJ与槲皮素，混合配制成不同浓度的溶液，以清水处理为对照。

But up to now , there is no report about the difference in the content of DNJ between different mulberry species or varieties .

关于我国不同桑种间1-脱氧野尻霉素的含量检测与分析未见报道。

DNJ is abundant in sericultural resources .

自然界中蚕桑资源中的DNJ含量最为丰富。

The macroporous H-103 resin adsorption technology was further employed to investigate the possibility for effectively separation of flavonoids and DNJ .

本文进一步考察了采用H-103型大孔树脂使桑叶粗提液中的黄酮与生物碱高效分离的可行性。

CONCLUSION This method is simple , rapid , accurate , sensitive and applicable for the determination of DNJ in Ramulus mori and it products .

结论方法准确灵敏、快速简便，可用于桑枝及其制品中DNJ含量的大数量样本测定。

Two kinds active substances all have obvious growth inhibition rate to the plutella xylostella larvae , the effect of quercetin is slightly less than DNJ .

两种活性物质均对小菜蛾幼虫具有明显的生长发育抑制率，槲皮素效果略差于DNJ。

The DNJ can be accumulated in Ramulus mori .

DNJ在桑枝中具有一定的累积作用。

The differences among different organizations are obvious , digest tipe and blood much higher than other organizations . Silkgland and fat body almost have no DNJ .

蚕体不同组织、器官中DNJ的含量存在很大差异，消化管和血液中的含量明显高于其他组织器官，丝腺和脂肪体中几乎检测不出DNJ。

There was rich DNJ in Ramulus mori , and the DNJ contents were significantly different according to the mulberry varieties , stage and ages .

桑枝富含DNJ，其含量在桑品种、生长季节和老嫩程度间存在显著差异。

DNJ and quercetin have the same effect of contact toxicity and stomach toxicity to experiment insect , and the mortality of stomach toxicity is higher than contact toxicity .

DNJ与槲皮素对试虫触杀与胃毒作用等效，且胃毒作用下的死亡率高于触杀作用。

In this study some factors associated with the contents of DNJ were investigated to provide a better understanding for the produce of DNJ by tissue or cell culture and the synthesis mechanisms of it .

有鉴于此，本文探讨了不同影响因子对组培桑苗中DNJ含量的影响，旨在为以后通过组织或细胞培养生产DNJ及探讨DNJ的合成机理提供参考。

1-deoxynojirimycin ( DNJ ) and its ramifications are effective α - glucosidase inhibitors , they are used in curing diabetes , fatness and virus infections .

1-脱氧野尻霉素（DNJ）及其衍生物是一种高效的α-葡萄糖苷酶抑制剂，可用于治疗糖尿病、肥胖症、病毒感染等疾病。

Coarse extract NAGase from spodoptera exigua larvae , DNJ have a obvious inhibition in NAGase activity , confirm DNJ is a-glycosidase enzymes inhibitors .

从甜菜夜蛾幼虫体内进行NAGase粗提取，DNJ对NAGase活性具有明显的抑制作用，印证了DNJ为α-葡萄糖苷酶抑制剂的特性。

Mulberry contains DNJ and quercetin and so on many kinds of biological active substance , can effectively restrain glycosidase activity , can greatly reduce lepidoptera families to the harm of crop pests .

桑树中含有1-脱氧野尻霉素（DNJ）和槲皮素等多种生物活性物质，能有效抑制糖苷酶活性，可大大降低鳞翅目科害虫对作物的危害。

Because of the existence of GABA and DNJ , mulberry leaves are the ideal materials for health care foods such as mulberry-tea , mulberry leaf juice , mulberry leaf noodle and mulberry leaf powder .

它含有功能食品因子γ-氨基丁酸（GABA）和1-脱氧野尻霉素（DNJ），可作为研制桑叶茶、桑叶汁饮料、桑叶面条、桑叶末（粉）等功能性保健制品的理想原料。

In addition , the type of DNJ inhibition of hypoglycemic activity was investigated by Lineweaver-Burk method , and the results showed that DNJ as a α - glucosidase inhibitor displayed a mixed competitive inhibition mechanism . 4 .

通过Lineweaver-Burk法考察了DNJ降血糖活性的抑制类型，结果表明DNJ对蔗糖底物和麦芽糖底物的α-葡萄糖苷酶抑制类型均为混合竞争性抑制。

There was rich DNJ in the silkworm larval body and the content of it was 0.448 % , which was higher than mulberry leaves and the content of DNJ was obvious different with variety , gender and the phrase of growth .

家蚕体富含DNJ，其含量为0.448%，大大高于过去认为富含DNJ的桑叶等植物体中的DNJ含量。

The test is to compare the weight of the crude extract in anhydrous ethanol , acetone , n-butanol , ethyl acetate and tetrachloromethane , and then using liquid chromatography ( HPLC ) to measure the DNJ and quercetin content in each crude extract .

利用无水乙醇、丙酮、正丁醇、乙酸乙酯和四氯甲烷五种溶剂浸提原料，优化浸提方法，比较粗提物的重量并利用液相色谱法分析各粗提物中DNJ和槲皮素含量。