5-azacytidine

5-Azacytidine Inhibit the Methylation Status of Interleukin-6 Promoter and Increase It 's Secretion in T Cells

5-氮杂胞苷对IL-6启动子DNA甲基化的影响

5-Azacytidine actives three types of K ~ + currents during MSCs proliferating and differentiating processes

5-氮杂胞苷促大鼠MSCs增殖及分化过程中的3种钾电流

Myocardial Differentiation of Human Bone Marrow Stromal Cells Induced by 5-Azacytidine

5-氮杂胞嘧啶诱导人骨髓基质干细胞心肌分化

Differentiation of bone marrow mesenchymal stem cells to cardiomyocytes in vitro with different concentration of 5-azacytidine

不同浓度5-氮胞苷对骨髓间质干细胞体外诱导分化的作用

Interaction of Arsenic and 5-Azacytidine on Damaging DNA and Inhibiting Repair in Human Lymphocytes

砷与5-氮胞苷对人淋巴细胞DNA损伤的联合作用

5-azacytidine can induce bone marrow mesenchymal stem cells to differentiate into muscle-like cells .

5-氮杂胞苷可以促使骨髓干细胞定向分化为肌样细胞。

Induction of Caspase 8 by 5-Azacytidine renders NB cells sensitive to TRAIL

5氮杂胞苷诱导NB细胞Caspase8表达及对TRAIL敏感性的研究

MATERIAL AND METHODS : After treatment of Bcap-37 cells with 5-azacytidine , cell proliferation was analyzed by cell counting .

材料与方法不同浓度的5-氮杂胞苷与Bcap-37细胞共培养后，应用细胞计数法检测Bcap-37细胞生长的情况；

The Effect of 5-azacytidine to the DNA Methylation and Morphogenesis Character of Chrysanthemum During in Vitro Growth

离体条件下5-氮杂胞嘧啶核苷对菊花DNA甲基化和表型性状的影响

Objective To study cardiac differentiation of bone marrow mesenchymal stem cell ( BMMSC ) induced by 5-azacytidine .

目的观察5-氮胞苷对骨髓间质干细胞（BMMSC）体外向心肌细胞诱导分化的影响。

Effects of 5-azacytidine on expression of β - adrenoceptors of cultured rat bone marrow stromal cells in vitro

5-氮胞苷诱导大鼠骨髓基质细胞表达β肾上腺素能受体的研究

Synergistic induction of apoptosis in neuroblastoma cells using a combination of 5-Azacytidine with chemotherapeutic drugs and TRAIL

去甲基药、化疗药及TRAIL联合诱导神经母细胞瘤细胞凋亡的研究

Toxicological genetics of the 5-azacytidine in inducing adipose-derived mesenchymal stem cells differentiate into myocardial cells

5-氮胞苷诱导脂肪间充质干细胞向心肌细胞分化的毒理遗传学研究

Effect of 5-azacytidine and simulated biological microenvironment on differentiation from bone marrow mesenchymal stem cells into myocardial-like cells

化学诱导剂5-氮杂胞苷及模拟生物微环境对骨髓间充质干细胞分化成心肌样细胞的影响

Conclusion It is preliminarily confirmed that human MSCs in vitro can be induced by 5-azacytidine into cardiomyocytes .

结论：初步证实人骨髓干细胞在体外被5-aza诱导时，能够向心肌细胞分化。

Methods : Mesenchymal stem cells were recovered from a ficoll gradient and treated with 5-azacytidine in vitro .

方法：离体实验中，利用ficoll进行密度梯度离心获得间充质干细胞后用5-氮胞苷诱导其分化。

Pretreatment with DNA methyltransferase inhibitor 5-azacytidine suppressed SNP-induced isoflavone production .

DNA甲基转移酶抑制剂5&氮杂胞苷阻断了一氧化氮激发下异黄酮合成。

Bone marrow-derived mesenchymal stem cells from sternum were cultured and differentiated after induced by 5-azacytidine .

由其胸骨获得骨髓间充质干细胞，进行培养并经5-氮杂胞苷诱导分化为肌源性细胞。

Differentiation of monolayer-cultured P19 cells toward cardiomyocytes induced by 5-azacytidine

5-氮胞苷诱导单层P19细胞向心肌分化

Angiotensin II can induce ADSCs to cardiomyocyte-like cells and can replace the traditional induction agent 5-azacytidine .

血管紧张素Ⅱ能诱导人脂肪干细胞向心肌样细胞分化，可以用来替代传统的诱导剂5-氮胞苷。

AIM : To study morphological and molecular biological feature of differentiation of umbilical cord blood stem cells in vitro after induced by 5-azacytidine into cardiomyocytes .

目的：观察脐血间充质干细胞经5-氮杂胞苷诱导在体外定向分化为心肌样细胞的形态学及分子生物学特征。

Objective To analyze the features of P19 cells differentiating into cardiomyocyte after induced by 5-azacytidine ( 5-aza ) in vitro .

目的探讨P19细胞体外经5-氮胞苷（5-aza）诱导向心肌细胞分化的特征。

Results Mesenchymal stem cells were spindle-shaped and active proliferation capacity in vitro , they can be differentiation into cardiac-like muscle cells after adding 5-azacytidine .

结果大鼠骨髓基质细胞贴壁呈集落生长，5-氮胞苷诱导骨髓基质细胞转化为心肌样细胞。

Methods Porcine bone marrow mesenchymal stem cells were induced by 5-azacytidine in vitro , the expression of cardiac troponin I ( cTnI ) was identified by ELASA method .

方法用5杂氮胞苷（5-azacytidine）体外诱导猪骨髓间质干细胞使之向心肌分化；ELASA法测定分化前、后细胞内心肌肌钙蛋白I（cTnI）变化；

TPA and 5-Azacytidine enhanced the effects of PHA , Actinomycin D inhibited but Hydroxyurea and Colchicine had no influence on it .

TPA、5-氮胞苷可增加PHA的作用，羟基脲和秋水仙素不影响，而放线菌素D可抑制pHA的效应。

The differentiate towards myocyte of MSCs after induced by 5-azacytidine in vitro indicates that MSCs are multipotential stem cell and be of specific function of expressing myocyte .

体外采用5-aza诱导下有部分MSCs向肌细胞分化，证实MSCs为多潜能干细胞，具有表达肌细胞的特异性功能。

To test whether DNA methylation plays a role in somaclonal variation , the calli were treated with 5-azacytidine that reduces cytosine methylation by blocking activity of DNA methyltransferase .

为了检测DNA甲基化在体细胞克隆多样性中的作用，愈伤组织用5-氮胞苷进行处理，5-氮胞苷通过抑制DNA甲基转移酶的活性降低胞嘧啶的甲基化水平。

RT-PCR examination : Expressions of atrial natriuretic polypeptide , phospholamban and β - myosin heavy chain mRNA in MSCs appeared at weeks 4 and 5 after induced by 5-azacytidine .

RT-PCR检测：经5-氮杂胞苷诱导四五周骨髓间充质干细胞有心房利钠肽、受磷蛋白、β-肌球蛋白重链表达。

CONCLUSION : Serum from AMI can not induce BMSC to differentiate into cardiomyocytes , but it promotes BMSC differentiate into cardiomyocytes induced by 5-azacytidine and facilitate the differentiated cells to mature .

结论：单纯用AMI大鼠血清不能诱导BMSC向心肌细胞分化，但AMI血清能促进5-aza诱导的BMSC向心肌细胞分化，并促进分化的心肌细胞成熟。

CONCLUSION : MSCs cultured for 4 weeks after incubation with 5-azacytidine or for 8 weeks without incubation both can directionally transform into myoid cell with myofilament structure , but not cardiogenic cells .

结论：骨髓间质干细胞经5-氮胞苷诱导培养4周及未诱导连续培养8周均可定向转化为具有肌丝结构的肌样细胞，而非心肌样细胞。