酶标法

建立双抗体夹心酶标法（ELISA）检测人血清中游离E受体的研究

Establishment of Sandwich ELISA for the Detection of Free-Form E-Receptors in Human Serum

β-HCG单克隆抗体酶标法对早期妊娠诊断的临床评价

Clinical Evaluation of the Urinary β HCG ELISA for Diagnosis of Early Pregnancy An Analysis of 375 Cases

采用吹气法或酶标法检测HP带菌情况。

By Purging or ELISA method for detection of HP infection .

方法采用免疫萤光标记直接法、免疫组化ABC酶标法对40例三叉神经脱髓鞘病变进行检测。

Methods We did the experiment by immunofluorescence labelling direct method and immunohistochemistry ABC enzyme labelling method .

血清MMP-9、TIMP-1均采用酶标法，按说明书操作。

Serum MMP-9 and TIMP-1 were determined by ELISA method according to the procedures of specification .

采用瑞典PharmaciaCAPSystem荧光酶标法测定ECP。

And eosinophil cationic protein ( ECP ) was measured by fluorescence enzyme labeled method with Sweden Pharmacia CAP system .

方法：分别用定量聚合酶链反应法（PCR）和酶标法（ELISA），检测207例乙型肝炎病毒慢性感染者血清HBVdna含量与乙肝病毒血清标记物（HBVM）；

Methods : HBV DNA quantity was detected by PCR and HBVM was detected by ELISA in 207 patients with chronic hepatitis B virus infection .

结果发现：ABC法与亲和酶标法测定的ER阳性符合率为91.2%。PR为82.8%；

It is suggested that ER-positive rates of conform of ABC with the affinity enzyme labeled methods were 91.2 % , PR were 82.8 % ;

RAPD标记可能用于白血病的基因分型，微小残留病检测等研究。间接免疫酶标法检测白血病细胞表面的运铁蛋白受体

Conclusion : The RAPD markers might be potential test in detection of minimal residual disease and leukemic genetic classification . TRANSFERRIN RECEPTORS ON LEUKEMIC CELLS

本文采用间接免疫酶标法检测了33例慢性肝病患者肝组织内HBeAg，其中26例慢性肝炎患者肝组织标本中，7例（26.9%）检出HBeAg；

Indirect immunoenzymatic technique has been employed to detect HBeAg in liver tissues of 33 patients with chronic liver diseases .

方法采用荧光酶标法（MAST法）检测了987例变态反应性皮肤病患者的过敏原血清特异性IgE（sIgE）和总IgE水平。

Methods 987 patients with common allergic dermatosis were detected the sIgE and total serum IgE level by MAST CLA-1 test system .

方法采用APAAP桥联酶标法测定外周血T细胞亚群。

Methods T lymphocyte subpopulation in peripheral blood was measured by APAAP method .

与DCC法对照，总符合率达92.5%和95.8%，且特异性检测发现酶标法是一种专一性较强的检测技术。

In comparing with the DCC method , the consistent rates were 92.5 % for ER and 95.8 % for PR.

改良双抗体酶标法用于人IgD的测定

Innovation of Double Antibody Sandwich Enzyme Immuno-assay for Determination of Human IgD

直接酶标法检测肝组织中丙型肝炎病毒抗原（HCAg－NS3）的研究

Detection of HCV antigen-ns_3 in liver tissue with monoclonal anti - HCV by direct enzyme immuno-assay

方法：应用APAAP桥联酶标法测定OSAS组和正常对照组外周血淋巴细胞表型，并将结果与睡眠检测指标进行相关性分析。

Method : Lymphocytes in peripheral blood vessel of children with OSAS vs normal controls were checked by APAAP method and analyzed statistically .

首次报告测定人血清中游离E受体的双抗体夹心酶标法（ELIsA），本法特异性强重复性好。

Establishment of a sandwich ELISA for detection of free-from E-receptors in human serum is reported . The specificity of this method was confirmed with inhibition test and the coefficient variance was 9.4-14.7 % , indicating good reproducibility of the assay .

方法：应用单克隆抗体免疫组织化学酶标法对确诊为IgA肾病患者的肾组织进行染色，依据沉积类型并按半定量方法结合病情进行分析。

Methods : Renal tissue staining was performed by immunohistochemical method in patients with IgA nephropathy , results were analyzed according to deposition pattern and semiquantitative determination in association with condition of the disease .

方法采用酶标法测定冠心病组（120例）和对照组（120例）血清CP特异性抗体IgM。

Methods Enzyme link immunosorbent assay ( ELISA ) methods were adapted of examine the specific antibody IgM of CP in plasma . 120 CHD patients and 120 healthy volunteers were involved in the study .

方法用酶标法检测75例慢性乙型病毒性肝炎和40例乙肝后肝硬化患者血清中MMP9浓度。

MethodsSerum MMP-9 was detected by ELISA in75 patients with CHB and40 patients with LC and20 healthy persons .

方法：用APAAP免疫酶标法检测10例经IL－2激活的急性白血病患者骨髓细胞相关抗原标记及活化过程中的变化。

Methods : 10 patients with acute leukemia were selected and their bone marrow cell related antigen labeling and activation process changes activated by IL-2 were detected by APAAP method .

方法采用非放射性同位素端粒酶重复扩增酶标法，对18例正常口腔粘膜上皮细胞端粒酶活性进行检测，同时用免疫组化方法检测正常口腔粘膜Ki67的表达。

Methods A non radioisotopic telomeric repeat amplification protocol assay was performed in normal oral mucosas .

抽取其外周血，采用单克隆抗体APAAP桥联酶标法进行T淋巴细胞亚群CD3+、CD4+、CD8+细胞检测。

Vein blood samples were taken and analyzed with monoclonal antibody APAAP bridged enzyme linked Immunosorbent assay to measure the percentage of CD3 + ? CD4 + and CD8 + T-cells .

方法采用桥联酶标法（APAAP）和酶联免疫吸附试验（ELISA）检测20例患儿外周血单个核细胞（PBMC）呼吸道病毒抗原和血清病毒抗体。

Methods Using APAAP and ELISA , the authors assayed the expression of respiratory tract viruses antigen in peripheral blood mononuclear cells ( PBMC ) and the production of viruses antibody in serum from 20 cases of children with steroid sensitive and simple nephrotic syndrome .

甲状腺球蛋白单克隆抗体酶标法的建立和应用

The Use of Thyroglobulin Monoclonal Antibody Enzyme-linked Immunosorbent Assay

单克隆抗体夹心酶标法检测鞭毛抗原快速诊断伤寒

Rapid diagnosis typhoid fever by detecting flagellin of salmonella typhi with McAb ELISA

抗结核菌抗体测定，使用酶标法。

Antituberculin antibody was determined by enzyme labelling .

用单克隆抗体桥联酶标法诊断小儿呼吸道病毒感染

Rapid diagnosis of children with respiratory tract viruses infection by monoclonal antibody bridge-linked ELISA

以免疫酶标法研究实验性高胆固醇血症时淋巴细胞低密度脂蛋白受体活性变化

Using enzyme immunoassay method to study the LDL receptor activity of lymphocyte in hypercholesterolemia

免疫荧光法和免疫酶标法检测新城疫病毒的研究

Determination of NDV with Immunocytochemical and Immunofluorescence Method