骨髓涂片

本文报告了用DNA区分染色法分析100例急性白血病初治前骨髓涂片的结果。

The result of cytological DNA differential stain in 100 cases of bone marrow smear in untreated acute leukemia is reported in this paper .

骨髓涂片细胞形态学误诊分析及对策&2000～2005年骨髓细胞形态学室间质量评价分析

Analysis and countermeasures of misdiagnosis on cell morphology of bone marrow smear

骨髓涂片微量融合基因RNA的RT-PCR分析

Analysis of fusion genes RNA from glass slide smears using RT PCR

骨髓涂片检查表现为二系以上造血细胞DNA复制紊乱的病态造血；

Bone marrow smears of MDS-RA patients show morbid hematopoiesis of above two lineages of hematopoietic cells for DNA copying confusion .

骨髓涂片和切片相结合有助于MM的诊断和分型。

Combination of bone marrow biopsy with bone marrow smear benefit the diagnosis and typing of MM .

以Chelex~R100作为介质、一步法从贮存骨髓涂片中提取DNA作PCR分析

Utilizing Chelex ￣ R 100 as A Medium for One-step Extraction of Dna From Stored Bone Marrow Smears for Use With PCR

方法回顾性总结经骨髓涂片或局部病灶活检病理证实的脊柱多发骨髓瘤17例，脊柱转移瘤32例，分析二者的MRI特点，归纳其影像表现的差异。

Methods The MRI of 17 cases of MM and 32 cases of metastases proved by histology in the vertebral column were analyzed retrospectively .

MRP在急性白血病患者骨髓涂片中表达的临床意义

The clinical significance of MRP protein expression in bone marrow smears of patients with acute leukemia

从贮存骨髓涂片提取RNA检测骨髓增生异常综合征病人的WT1基因表达

Extraction of RNA from Stored Bone Marrow Smears for Detecting Expression of the WT1 Gene in MDS Patients

材料与方法：经骨髓涂片或活检、免疫学检查，确诊为IgG型MM患者16例，对其X线及CT影像进行分析及总结。

Materials and Methods : The Xray and CT features of 16 cases with IgG multiple myeloma , proved by immunological test and bone marrow aspiration or biopsy , were analyzed .

骨髓涂片中MRP表达阳性患者化疗缓解率54.5%低于MRP表达阴性患者95%，具有统计学差异。

The CR rate of chemotherapy was lower in patients with MRP expression than that in patients with negative expression ( 54.5 % vs95 % ) .

其中8例无明显浅表淋巴结肿大者经外周血、骨髓涂片及切片联合检查确诊为NHL；

In 8 of the 28 cases without superficial lymphadenopathy , the diagnosis of NHL was established through bone marrow and peripheral blood examination .

方法：采用聚合酶链反应（PCR）技术，对32例小儿急性淋巴细胞白血病骨髓涂片标本，进行免疫球蛋白IgH和T细胞受体r链基因重排的检测。

Method : Thirty-two marrow smear samples of the children suffering acute lymphocyte leukemia were used to detect the recombinations of immunoglobulin IgH and r-chain genes of T cell receptor by PCR .

方法对27例急性白血病化疗后骨髓涂片首次完全缓解（CR）时的骨髓切片进行同步观察，计数单位面积内原始细胞簇及原始细胞总数。

Methods We studied 27 cases of AL by observing their bone marrow ( BM ) biopsy sections and simultaneously , their BM smears from which the first complete remission ( CR ) were determined after chemotherapy .

方法：利用形态学及细胞化学染色检查94例CML患者和63例类白血病反应患者的骨髓涂片；

Methods : We examined 157 bone marrow slides ( 94 from patients with CML and 63 from patients with leukemoid reaction ) by morphometry and cytochemistry stain .

方法对12例GS的组织形态学及免疫表型特征进行研究，并结合外周血、骨髓涂片检查及骨髓活检对全部病例进行FAB分型诊断。

Methods The morphological and immunological characteristics of 12 cases of GS were studied . FAB classification was made by peripheral blood , bone marrow picture and bone marrow biopsy assay .

研究表明，kit基因突变是多数肥大细胞增生症的病因，血浆类胰蛋白酶检测及骨髓涂片CD25免疫组化标记对诊断系统性肥大细胞增生症有重要意义。

It has been proved that the main cause of mastocytosis is the mutation of kit gene . Determination of tryptase levels in plasma and CD25 immunohistochemistry of bone marrow are of great importance to diagnose mastocytosis .

目的研究骨髓涂片细胞形态学室间质量评价（EQA）方法、标准，探讨其临床应用价值。

Objective To establish an external quality assessment ( EQA ) scheme for cell morphological interpretation of bone marrow smears and investigate its application value .

方法观察42例CMML病人的骨髓涂片与骨髓活检切片，并做出比较和分析。

Methods The bone marrow biopsy of42 patients with CMML were examined and the results were analyzed .

方法以Chelex100为介质，从初诊的ALL患儿骨髓涂片中提取DNA，借助聚合酶链反应（PCR）技术检测IgH、TCRβ、TCRδ和TCRγ基因重排。

Methods After extraction of DNA from stored bone marrow smears at diagnosis with chelex 100 , IgH , TCR β, TCR δ and TCR γ gene rearrangements were detected by using polymerase chain reaction ( PCR ) technique .

结果：94例CML患者的骨髓涂片中有29例找到类高雪氏细胞，而类白血病反应患者的骨髓涂片中未找到类高雪氏细胞；

Results : Pseudo-Gaucher cells were found in the bone marrow in 29 of 94 cases with CML ; however , it was not found in the bone marrow in 63 cases with leukemoid reaction .

探讨利用常规储藏的骨髓涂片进行RNA分析的可能性。利用RT－PCR法对6例具有不同融合基因的白血病患者的RNA转录本进行检测。

In order to explore the possibility of using routinely prepared glass slide smears of bone marrow as sourse of cellular material for fusion genes RNA analysis , RT PCR was used to detect RNA transcripts in 6 untreated leukemia patients with specific fusion genes .

骨髓涂片巨核细胞数正常组的患者CFUMeg为19.43±7.28和BFUMeg为4.67±1.53，均低于对照组。

In patients with normal number of megakaryocytes on marrow smears , CFU-Meg and BFU-Meg were 19.43 ± 7.28 and 4.67 ± 1.53 , respectively , the values were lower as compared to control group .

用McAb-AST及骨髓涂片法对四川省汶川县犬感染利什曼原虫的调查研究

A Survey of Dog Infected with Leishmania Donovani in Wen-chuan County of Sichuan Province by McAb-AST and Bone-Marrow Smear Methods

从常规保存的骨髓涂片抽提DNA为模板，应用聚合酶链反应（PCR）技术检测多发性骨髓瘤（MM）与反应性浆细胞增多症（RP）IgH基因重排方式。

Using the DNA extracted from bone marrow smears which have been stored routinely as a template , polymerase chain reaction ( PCR ) was utilized to detect clonal immunoglobulin heavy chain ( IgH ) gene rearrangements in multiple myeloma ( MM ) and reactive plasmacytosis ( RP ) .

应用多聚酶链反应技术检测15例急性淋巴细胞白血病患者缓解期骨髓涂片中Ig基因重排，其中有5例（33%）检测出残留白血病细胞。

The rearrangement of immunoglobin genes in the bone marrow slides of 15 cases of acute lymphocytic leukemia in remission stage was studied with PCR . It was found that 5 cases out of the 15 ( 33 % ) demonstrated residual leukemic cells .

结论以细胞形态学检查为主，结合临床资料，全面综合分析作出实验室诊断的骨髓涂片EQA体系，能有效提高血液病细胞形态学检查技术与诊断水平。

Conclusion The system of external quality assessment in which the bone marrow cell morphology serves as the main examination means and the clinical data are combined to make the experimental diagnosis after the comprehensive analysis can effectively improve the level of diagnosis and treatment of the hematological diseases .

120例感染患者骨髓涂片细胞形态观察

The morphologic study of 120 bone marrow smears of infected patients

骨髓涂片和活检均可见较多的大颗粒淋巴细胞浸润；

Large granular lymphocytes infiltrated in bone marrow aspirate and core biopsy ;

方法：选取44例急性淋巴细胞白血病患者的新鲜骨髓涂片。

Methods : Select 44 cases of ALL verdure bone marrow smears .