差速离心

方法依次使用差速离心、热失活、离子交换层析的方法纯化体外重组的人tau蛋白；

Methods Human recombinant tau protein in vitro was purified by using differential centrifugation , heat inactivation and ion exchange chromatography .

该方法利用差速离心分离植物细胞核，经低熔点琼脂糖块或低熔点琼脂糖微珠包埋，蛋白酶K原位裂解后制备大分子量核DNA。

Generally it includes isolation of purified nuclei by differential centrifugation , embedment of the nuclei in either low-melting-point agarose plugs or microbeads , and digestion with proteinase K in situ to release high molecular weight ( HMW ) DNA .

方法：大鼠经皮下注射Iso共10天以制备心肌纤维化模型，心肌线粒体以差速离心法分离。对照组仅注射无血清的培养液。

The control group was the model rats received only DMEM injection .

同时还比较了差速离心、PEG沉淀、PEG包埋等方法。

Many other methods such as PEG precipitation , differential speed centrifugation PEG covering were progressed .

方法：SD大鼠，雄性，断头处死后取肝脏制成匀浆，差速离心法制备肝微粒体。

Methods : SD rats , male , were used to prepare the rat liver microsomes by using differential ultracentrifugation .

应用差速离心法提取脑皮质线粒体，Clark氧电极法测定线粒体呼吸功能，及细胞色素C氧化酶（COX）的活性。

Mitochondrial respiratory function and COX activity were measured by Clark oxygen electrode . Mitochondria were obtained by differential centrifugation .

方法差速离心法制备Beagle犬肝微粒体酶，0.4μmol。

Methods Beagle dog liver microsomes were prepared by using ultracentrifuge method .

该方法以大白菜球叶为材料，采用差速离心和蔗糖衬垫相结合的方法分离出线粒体，然后用SDS&蛋白酶K裂解法提取mtDNA。

The mitochondria was extracted from the inner leaves of CMS lines and their maintainer lines by using differential centrifugation and sucrose shelf method , then the mtDNA was extracted by SDS-Proteinase K method .

采用差速离心法及DNaseⅠ、RNase消化法制备并纯化了鲇（SilurusAsotus）肝脏线粒体DNA（mitochondrialDNA，mtDNA）。

The mitochondrial DNA ( mtDNA ) from liver of Silurus asotus had been isolated and purified by the method of density gradient centrifugation and DNase I , RNase digestion .

对差速离心纯化的汉坦病毒R84-1毒株进行了SDS-PAGE和免疫印迹试验。

SDS-PAGE and immunoblotting test of Hantavirus ( HTV ) R84-1 strain purified by differential centrifugation were carried out .

采用差速离心法制备大鼠肝微粒体，测定细胞色素P450、b5含量及苯胺羟化酶活力。

The liver microsomal fraction was prepared by differential centrifugation and the activity of liver microsomal enzymes P450 , b5 and aniline hydroxylase in rats were studied .

方法低温差速离心分离肝癌亚细胞组份，应用NAA法检测各亚细胞组份的微量元素含量，然后与正常人肝及癌旁组织的相应亚细胞组份相比较。

Methods The distribution pattern of 17 elements in subcellular fractions of human hepatocellular carcinoma was studied by differential centrifugation combined with NAA , then compared to the human liver with t-test .

通过差速离心法和蔗糖密度梯度超速离心后所得的提纯病毒样品，用紫外分光光度法检测可见典型的病毒核蛋白吸收曲线，用SDS-PAGE分析和电镜观察也证明该样品为病毒纯品。

The purified virus sample gained by sucrose density gradient centrifugation was proved to be typical absorbance curve of virus nucleoprotein and pure virus by methods of SDS-PAGE and electron microscopy and so on .

方法：采用差速离心和蔗糖衬垫相结合的方法先从大白菜胞质雄性不育系及保持系叶片中分离出线粒体，然后用SDS&蛋白酶K裂解法提取mtDNA。

Methods : Based on differential centrifugation and sucrose shelf method , the mitochondria was extracted from cytoplasmic male sterile lines and their maintainer lines of Chinese cabbage and the mtDNA was extracted successfully by SDS-Proteinase K method .

以猪脑为材料，经匀浆、差速离心、蔗糖密度梯度离心分离突触体.Percoll密度梯度离心分离牛精子初探

Synaptosomes were isolated from pig brain by homogenization , differential centrifugation and sucrose gradient centrifugation . Study on Bull Sperm Separation by Percoll Density with Centrifuge

运用差速离心法和化学试剂逐步提取法分析了Cu在鸭跖草Commelinacommunis根、茎、叶的亚细胞分布及贮存形态。

The differential centrifugation technique and sequential chemical extraction method were used to study the subcellular distribution and the chemical forms of Cu in the roots , leaves and stems of Commelina communis .

病毒性出血症病兔肝悬液离心去杂后其上清用聚乙二醇浓缩，又经正丁醇-异戊醇除去杂蛋白，然后以差速离心和蔗糖梯度离心，提取兔出血症病毒（RHDV）。

Rabbit haemorrhagic disease virus ( RHDV ) was purified from the infected rabbit livers by using the mixture of n-butanol and isoamyl alcohol , combined with sucrose density gradient centrifugation .

单斑分离接种昆诺阿黎做为提纯材料，经PEG（分子量6000）沉淀，差速离心及蔗糖密度梯度离心，病毒提纯量约140毫克/公斤鲜组织。

But other five species plants of two families tested were not infected . After two cycles single lesion isolation , the virus yield purified from C. quinoa by PEG ( MW 6,000 ) precipitation and sucrose gradient density centrifugation was about 140 mg / Kg fresh tissues .

分离物85-1在曼陀罗上繁殖，经PEG沉淀，两次差速离心和蔗糖梯度离心后的得毒率为0.1mg/kg材料。

The isolate 85-1 was propagated on Datura stramonium and purified by PEG precipitation , two cycles of differential centri - fugation and sucrose density gradient centrifugation . The virus yield was 0.1 mg / kg of fresh plant material .

尿囊液毒穴36～48h雪经差速离心和不连续蔗糖密度梯度离心纯化，在电镜下能观察到典型的冠状病毒形态，病毒粒子直径为80～120nm，有囊膜、纤突。

The allantoic fluid ( between 36 ~ 48 hours ) was purified by difference speed centrifugation and discontinous centrifugation on sucrose gradients . The spherical virus in 80 ~ 120nm diameter and with a surface covered by spike like corona were observed under electron microscope .

细胞捣碎后，通过差速离心和不连续蔗糖密度梯度离心获得富集液泡膜的膜微囊。

Cells by differential centrifugation and discontinuous sucrose density gradient centrifugation .

以健康对虾的鳃组织为材料，经过差速离心后获得对虾鳃细胞膜。

Shrimp cell membrane was obtained from healthy shrimp gill through centrifugation .

以差速离心和蔗糖密度梯度离心提纯了该病毒，纯化病毒的A260/A280为1.26；

The virus is purified by differential and rate - zonal centrifugation .

试验方法采用多步差速离心和密度梯度离心联合法分离纯化肝脏线粒体。

Mitochondrial purification process using the two-step differential centrifugation and density gradient method .

大鼠心肌细胞核采用差速离心和密度梯度离心分离提纯。

Velocity and isopyknic gradient centrifugation was employed to fractionate rat myocardial nuclei .

用差速离心法分离绿色小麦叶片线粒体。

Wheat leaf mitochondria are isolated with differential centrifugation .

用氯仿澄清，聚乙二醇沉淀和差速离心可部分提纯。

The virus was purified by means of chloroform clarification , polyethyleneglycol precipitation and differential centrifugation .

通过差速离心和两次不连续蔗糖梯度离心成功分离纯化栉孔扇贝病毒样颗粒。

The virus-like particles of scallop ( Chlamys farreri ) were purified successfully by means of sucrose gradient centrifugation .

用差速离心法提取心肌线粒体，考马斯亮蓝法测定蛋白含量。

Myocardial mitochondria was extracted by differential centrifugation , and protein level was measured by Coomassie brilliant blue method .

差速离心法制备肝线粒体。以双电机同步联动控制系统为例，提出了一种差速负反馈同步联动控制方案。

In this paper , a speed-error feedback method is introduced based on the double-motor synchronizing drive control system .