ymdd

Objective HBV genotypes and YMDD mutations were detected by reverse dot blot .

目的应用逆向斑点杂交技术检测乙型肝炎病毒（HBV）基因型及YMDD变异。

The shorter time YMDD variant takes to become wild strain , the worse the prognosis will be .

YMDD变异株转变为野生株的时间越短，其预后可能越差。

Conclusion Detection of HBV genotypes and YMDD mutations together were economical , exact , rapid and easy .

结论在同一张尼龙膜上检测HBV基因型及YMDD变异，经济实惠、准确快捷，且易于开展。

Methods HBV P gene was amplified by PCR and the products was sequenced to analyze the YMDD mutation .

方法聚合酶链反应（PCR）扩增拉米夫定耐药患者的HBVP基因，产物经直接测序检测其YMDD变异；

Objective : To explore the possibility of a YMDD mutations in the lamivudine untreated patients with chronic hepatitis B.

结论：显示在未经抗病毒治疗的慢乙肝患者中存在YMDD变异株，其与野生株一样是自然存在。

OBJECTIVE To determine the relationship between YMDD mutations of hepatitis B virus ( HBV ) and HBV genotypes during lamivudine treatment .

目的探讨乙型肝炎病毒（HBV）发生YMDD基因变异与HBV基因型的关系。

Results PCR , restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD , YVDD and YIDD were constructed successfully .

结果经PCR方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功，且该方法具有较好的敏感性和特异性。

Methods : Using direct sequencing , YMDD ( tyrosine-methionine-aspartate-aspartate ) variants in patients with chronic HBV were detected before and during treatment with lamivudine .

方法：用PCR产物直接测序检测拉米夫定治疗慢乙肝前后的YMDD变异，分析耐拉米夫定耐药株的分布及临床表型。

YMDD mutation of PCR melting curve and ALT and AST and HBV-DNA fluorimetry quantitative assay play an important role in the diagnosis and treatment of chronic hepatitis B .

PCR熔解曲线法测定YMDD变异株与ALT和AST及HBV-DNA荧光定量联合检测对慢性乙型肝炎患者的诊断，治疗有极其重要的价值。

Methods The serum YMDD mutation , HBV DNA , ALT and HbeAg levels of60 patients on lamivudine therapy were detected by FQ-PCR , ELISA , and rate method , respectively .

方法采用实时荧光定量PCR、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBVDNA、乙肝标志物及ALT的变化情况。

Emergence of resistant strains with amino acid substitutions in the tyrosine-methionine-aspartate-aspartate ( YMDD ) motif of reverse transcriptase is a serious problem in patients on LAM therapy .

严重困扰接受拉米夫定治疗患者的问题是，逆转录酶中的酪氨酸-蛋氨酸-天冬氨酸-天冬氨酸（YMDD）序列可能发生氨基酸置换突变，从而导致耐药菌株的产生。

Objective To probe into the existence of genovariation of RT district in HBV reverse transcriptase in the patients that are drug fast to Lamivudine and negative on YMDD variant .

目的探讨YMDD变异阴性的拉米夫丁耐药患者体内HBV逆转录酶RT区基因是否有变异。

Objective To establish a convenient method for detecting YMDD mutations in polymerase gene ( P gene ) of hepatitis B virus ( HBV ) and to evaluate the effect of lamivudine on these mutations .

目的建立简便易行的检测乙型肝炎病毒（HBV）多聚酶基因（P基因）上YMDD变异株的方法，评价运用拉米夫定治疗慢性乙型肝炎患者对此变异的影响。

To guide reasonably antival therapy in clinic and make therapic remedy , it is necessary for HBV-infected patients before or after antiviral therapy to detect HBV YMDD mutations .

HBV感染患者在治疗前后有必要检测是否存在HBVYMDD变异株感染，为指导临床科学合理用药和制定治疗方案提供依据。

Objective : To observe the efficacy and safety of adefovir dipivoxil ( ADV ) for chronic hepatitis B ( CHB ) with YMDD mutation during lamivudine ( LAM ) therapy .

目的：研究阿德福韦酯治疗YMDD突变后慢性乙型肝炎患者的疗效和安全性。