ultracentrifugation
- n.超速离心;超速离心法
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Methods : Biliary vesicules were isolated by ultracentrifugation , and their vesicular protein ( s ) isolated by SDS-PAGE and purified by HPLC . The nucleating activity was determined by the nucleation-time method .
方法:应用超速离心法分离胆石症患者胆汁泡,运用SDS-PAGE电泳分离其中胆汁泡蛋白,并经HPLC系统纯化,研究其成核活性和N-端氨基酸序列。
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Conclusion Modified ultracentrifugation is more suitable for preparing the sample of central nervous system .
结论改良超速离心法更适于制备来源于中枢神经系统双向电泳样品。
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This method is simple , needs only small amount of serum and no ultracentrifugation .
本法操作简便、血清用量小、不需超速离心。
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A program for processing of data from analytical ultracentrifugation
超离心分析数据处理软件包
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Data Processing of Sedimentation Velocity Experiment of Ultracentrifugation
超离心沉降速度实验数据处理程序
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Determination of Protein Sedimentation Coefficient by Sucrose Density Gradient Ultracentrifugation
蔗糖密度梯度离心法测定蛋白质的沉降系数
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A study of forming niobium colloid in nitric acid solution by ultracentrifugation
用超离心法研究硝酸溶液中铌胶体的形成
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The molecular weight was determined to be 280 000 by ultracentrifugation .
超离心分析测得相对分子质量为280000。
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Isolation by Density Gradient Ultracentrifugation and Radioiodination of Human Serum Low DenSity Lipoprotein
低密度脂蛋白密度梯度超速离心快速分离及~(125)Ⅰ标记的实验研究
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An Analytical Ultracentrifugation Method for the Measurement of Molecular Weight and Sedimentation Coefficient of Biological Macromolecules
分析超速离心法测定生物大分子的沉降系数和分子量
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Rapid Isolation of Large Amount of Plasma VLDL and LDL by a Two Step ultracentrifugation
两步超速离心法快速分离大量血浆极低密度脂蛋白及低密度脂蛋白
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This protein could be purified with CsCl and sucrose density gradient ultracentrifugation .
CsCl超速离心结合蔗糖密度超速离心可纯化此蛋白。
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Rapid fractionation of polysomes by density gradient ultracentrifugation in vertical tube rotor
用垂直转头密度梯度超速离心法快速分级分离多聚核糖体
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Purification of Japanese Encephalitis Vaccine by Ultracentrifugation with Sucrose Bed
蔗糖垫超速离心法提纯乙型脑炎疫苗
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Methods ( 1 ) Plasma lipoproteins were isolated and purified by ultracentrifugation .
方法(1)用超离心方法分离纯化血浆各脂蛋白;
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Methods : SD rats , male , were used to prepare the rat liver microsomes by using differential ultracentrifugation .
方法:SD大鼠,雄性,断头处死后取肝脏制成匀浆,差速离心法制备肝微粒体。
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The various lipoproteins in rabbit serum may be separated by ultracentrifugation in a vertical rotor at once .
用垂直转头超速离心法一次可制备兔血清各种脂蛋白。
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Methods : To isolate LDL by using polyanions precipitation and short-time density gradient ultracentrifugation .
方法:采用多聚阴离子沉淀结合短时密度梯度超速离心法得到纯化的LDL;
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The ser - um could be separated into 8 layers by potassium bromide density gradient ultracentrifugation .
经溴化钾密度梯度超离心分离大鼠血清得到8层制品。
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Conclusion : We can prepare the coating antigen in measles IgG EIA test system by continuous sucrose density gradient ultracentrifugation .
结论:采用超滤、PEG沉淀及连续蔗糖密度梯度超速离心的方法可制备满足麻疹IgGEIA诊断试剂要求的包被抗原。
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The bovine high density lipoprotein ( HDL ) was first isolated and purified in our country with zonal gradient ultracentrifugation .
本文采用区带密度梯度离心,国内首次分离得到黄牛纯化的高密度脂蛋白(HDL)。
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METHODS : TAF was purified at scheduled intervals by using extract methods such as hollow fiber column , ultracentrifugation , chromatography and electrophoresis .
方法:应用中空纤维超滤、超速离心、层析和电泳等方法,对肺癌及卵巢癌患者的胸腔积液、腹水中TAF进行提纯和分析;
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The results show that the density difference between particles and gradient medium , particle size and surface chemistry plays a major role in the density gradient ultracentrifugation rate separation .
研究表明在超离心过程中,颗粒与梯度介质密度差、颗粒尺寸及其表面化学对其在密度梯度里分离起主要作用。
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METHODS The liver microsomes were prepared by ultracentrifugation , the HMG CoA reductase activity was determined by radioactivity in vitro .
方法超速离心法制备肝微粒体,放射性同位素方法测定肝脏HMGCoA还原酶的活性。
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Studies on Effect of LiPoProteins and APoProteins on LiPoProtein Receptors ⅰ . Isolation of LiPoproteins and LDS by One Step Ultracentrifugation
脂蛋白及载脂蛋白对脂蛋白受体作用的研究&Ⅰ.一步超速离心分离血清VLD.LDL及部分HDL和LDS
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Monomeric and trimeric subcomplexes of LHC ⅱ were obtained by using sucrose gradient ultracentrifugation .
经蔗糖密度梯度超速离心获得了该色素蛋白复合体的单体和三聚体。
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The 3 % LM-solubilized fractions are separated by different methods : sucrose density gradient ultracentrifugation , anion exchange column , molecular size elution column .
对于3%LM增溶后的类囊体膜蛋白,分别采用了蔗糖密度梯度离心,离子交换柱层析和分子筛层析等分离纯化方法。
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Apolipoprotein C ⅰ, C ⅲ were isolated and purified by ultracentrifugation , gel filtration and high performance liquide chromatography from human serum very low density lipoprotein .
本文应用超速离心、凝胶过滤层析、高效液相色谱法分离纯化了人血清极低密度脂蛋白&载脂蛋白CⅠ和CⅢ。
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The encapsulation ratio of ORI-SLN was determined by ultracentrifugation method and the mean was 91.43 % with RSD of 1.25 % .
用低温高速离心法测定纳米粒的包封率,结果平均包封率为91.43%,相对标准差为1.25%。
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The disease-specific protein of rice grassy stunt virus ( RGSV ) was purified by differential pH precipitation and ultracentrifugation .
采用不同pH值沉淀法及超速离心获得提纯的水稻草矮病特异蛋白。