transpeptidation

Objective : An immobilized trypsin ( ITP ) is prepared for transpeptidation .

目的：制备固定化胰蛋白酶，用于转肽反应。

In addition immobilized enzyme was prepared , and we studied the immobilized enzyme-catalyzed transpeptidation .

另外本文还采用壳聚糖通过戊二醛固定酶，制得固相酶，催化人胰岛素酯的制备，研究固相酶促半合成人胰岛素的制备。

Porcine insulin was converted into human insulin ester with threonine-ester through transpeptidation catalyzed by enzyme .

本论文以猪胰岛素和苏氨酸叔丁酯为原料，通过酶促反应使B30丙氨酸换为苏氨酸叔丁酯，合成人胰岛素酯。

The activity of immobilized trypsin prepared through rapid technique reached 5990.5U/g and the whole process was more rapid and convenient than the technique before improvement , therefore it could be used for transpeptidation in industry successfully .

快速制备高活力的固定化胰蛋白酶，活力单位高达5990.5U/g，该工艺具有更为快捷简便的特点。