transformant

Melanin Study of the Transformant of Bacillus thuringiensis Containing Tyrosinase Gene

苏云金杆菌酪氨酸酶基因转化子黑色素的研究

With microbe morphologic and RAPD technique , we made a fringe identification of inherited background of the transformant .

应用微生物形态学和RAPD技术，对转化子的遗传背景进行鉴定。

The effects of different concentration of bacterium solution and infecting times on the transformant ratio were studied .

比较了不同菌液浓度和感染时间对转化率的影响。

The fimbria structure carried by the transformant strains was observed under electron microscope .

电镜下观察，转化的细菌在其表面形成菌毛样结构。

Through the determination of growth curve , showed that the transformant growed slowly . 5 .

通过对生长曲线的测定，转化后的灵芝生长缓慢。

Since the first rice transformant appeared in1980s , studies on rice transformation have undergone rapid strides .

自80年代第一株可育转基因水稻问世以来，水稻转化研究进展迅速。

Chloramphenicol could be used as selection pressure for positive transformant when cat gene was used as selectable marker gene .

当以cat基因作为选择性标记基因时，氯霉素可以作为紫菜原生质体基因转化的选择压力。

At last , two transformants were obtained . One is an unstable transformant , but it has obvious phenotype .

最终筛选得到2株转化子，一株转化苗为非稳定的转化子，但却具有明显的表型。

The apoptotic rate of anoikis-resistant cells was lower than that of the transformant at the same time point .

抗失巢凋亡细胞再次悬浮同时相凋亡率与转化株相比下降；

Southern blot analysis showed the hph gene has been integrated into genomic DNA of A. niger transformant .

抗性转化子的Southern分析表明，潮霉素磷酸转移酶基因已整合到受体黑曲霉的基因组DNA中。

The expressing products of CTX-M-38 transformant was detected by SDS-PAGE electrophoresis technique . 5 .

采用SDS-PAGE蛋白电泳技术，检测BL21-pET-28a-CTX-M-38转化子表达产物。

Influence of the Mutated proA Gene from Bacillus subtilis on Improving Osmotic Tolerance of Escherichia coli Transformant

枯草芽孢杆菌proA基因突变对提高大肠杆菌转化子渗透压耐受能力的影响

Objective The aim of this study was to obtain Trichoderma REMI transformant with double functions of disease prevention and phosphate-dissolving .

为了获得具有防病和解磷双重功能的木霉菌REMI转化子。

Construction of antisense PARP cDNA recombinants and establishment of the HeLa transformant after antisense plasmid introducing

反义PARP基因真核表达重组体的构建及其HeLa转化细胞系的建立

Reported in a typical experiment , 10 % ~ 20 % of the fertile injected flies produce transformant progeny .

据报道，在典型实验中，插入可育果蝇的10%～20%可产生转化体后裔。

This recombinant plasmid contains Pyrimethamine-resistant gene and report gene of green fluorescent protein , and can be expressed in transformant .

重组质粒含有息疟定抗性基因和绿色荧光蛋白的报告基因，可在转化子中表达。

Study on Novel Halophilous Characteristics of Penicillium chrysogenum vgb Transformant PTH-1

产黄青霉vgb转化菌PTH-1嗜盐新特性研究

Studies on Reversion of a Transformant Line and Loss of Exogenous ras Oncogene after Treatment with an Inhibitor of PARP Enzyme

PARP酶抑制剂诱导转化细胞逆转及外源ras癌基因丢失的研究

Isolate S2514 that obtained by screening REMI transformant library is a slow-growth mutant .

通过筛选稻瘟菌的REMI转化体库，获得一个生长缓慢型突变体S2514。

As genes are inserted randomly into the genome by Agrobacterium , each transformant must be assessed separately for the consequences of transformation .

另外因为基因是利用农杆菌随机地插入植物基因组中的，所以每个转化体必须对转化的序列进行单独分析。

Penicillin production of the transformant was analyzed by bioassays and the microbodies were observed by transmission electron microscope ( TEM ) .

同时通过透射电镜观察了菌体中微体数量的变化。

The transformant could keep a high percentage of plasmid-containing of transformant with 67.87 % after 50 generations in non-selective medium .

酵母转化子在非选择性培养条件下连续生长50代后，仍有67.87%的细胞保留该质粒。

We also tried the PEG-protoplast and Agrobacterium tumefaciens mediated method to transform R. cerealis , and no transformant was obtained .

并且尝试了PEG-原生质体转化和农杆菌介导的转化等方法，没有成功建立禾谷丝核菌的遗传转化体系。

Tagging the genes involved in trapping-device in nematode-trapping fungi ii . the morphology and Southern blot analysis of the transformant not forming trapping device

捕食线虫真菌捕食器相关基因标记II.捕食器基因不表达转化子的形态特征及核酸杂交分析

The gene cloning strategy was to construct genomic library after location of its neighbouring gene by Southern blot and to screen the aim transformant by dot blotting .

采用的基因克隆策略是用Southern杂交对其邻近基因进行定位后构建基因组文库，再用斑点杂交从基因文库中筛选目的转化子。

The penicillin V yield produced by transformant PTH - 1 was improved up to 1 . 1 4 - fold as compared with control in batch fermentation experiments .

在高盐发酵试验中，PTH-1产抗比对照株提高14%。

Under oxygen-limited condition , the transformation efficiency of the intermediate of levonorgestrel in transformant is 60.60 % , that of the contrast strain is almost 0 % .

贫氧条件下，对照菌左炔诺孕酮中间体转化率几乎为0%，而vgb基因转化子左炔诺孕酮中间体转化率为60.60%。

The experiment of the three varieties 's resistance to antibiotics finds that , the identified standard of the transformant is that the plant grows in gear , has green leaves or lateral roots or both ;

三个材料抗生素抗性试验结果表明：鉴别转化体的标准为植株生长正常，叶片绿色，有侧根发出或真叶长出或既有真叶长出又有侧根出现。

Results Susceptibility of transformant containing qnr gene against common fluoroquinolones was 3 ~ 25 times lower than recipient stain , but drug-resistance was 4 ~ 256 times lower than the clinical isolate .

结果qnr基因转化子对常见氟喹诺酮类抗菌药物的敏感性较受体菌下降3~25倍，但耐药水平低于临床分离菌株4~256倍。

Shake-flask fermentation under optimal conditions showed that glucoamylase secreted by the transformant GB0506 was17.5 % higher than parental strain F0410 at the end of fermentation .

对转化子进行摇瓶发酵研究，发酵终止时转化子GB0506的糖化酶活力比出发菌株F0410提高了17.5%。