tc3

β TC3 cells were also used to monitor insulin secretion .

另外检测βTC3细胞胰岛素的释放量。

Effect of oleic acid on PTEN mRNA expression in β TC3 cells

油酸对βTC3细胞PTENmRNA表达的影响

The Study of Effects and Mechanism of Pioglitazone on β Tc3 Cell Apoptosis Induced by Free Fatty Acid

吡格列酮对游离脂肪酸诱导的βTc3细胞凋亡的干预作用及其机制研究

Free fatty acids-induced β TC3 cell defects are associated with altered mitochondrial function

游离脂肪酸诱导的胰岛βTC3细胞缺陷与线粒体功能改变的相关性研究

Results : Apoptosis percentages of islet cells and β Tc3 cells increased significantly in high concentration glucose cultured medium .

结果：高浓度葡萄糖使原代培养的大鼠胰岛细胞和βTc-3的凋亡细胞比例明显增加；

The apoptotic proportions of rat islet cells and mouse β Tc3 cells were significantly increased at high glucose concentration .

高糖培养可以明显增加大鼠胰岛细胞和小鼠βTc3细胞凋亡百分率；

Conclusion : The high expression of PPAR γ 1 could protect β TC3 cells from FFA-induced impairment .

结论PPARγ1高表达可保护胰岛βTC3细胞免于FFA介导的损伤。

β TC3 cells were not stimulated by BER .

小檗碱没有刺激βTC3细胞胰岛素分泌的作用。

Step number Variable or selection TC1 TC2 TC3 TC4

步骤号变量或者选择TC1TC2TC3TC4

Methods : Apoptosis percentages of primary rat islet cells and β Tc3 cells cultured with high concentration glucose medium were measured by TUNEL method .

方法：应用TUNEL法检测高浓度葡萄糖培养后大鼠胰岛细胞和小鼠βTc3细胞凋亡百分率；

MRNA expressions of bcl-2 and bax of cultured rat islet cells and mouse β Tc3 cells were detected by QRT-PCR .

应用定量RT-PCR（QRT-PCR）检测培养后大鼠胰岛细胞和小鼠βTc3细胞bcl-2和BAXmRNA的表达；

However , at the same condition , the cell viability of the β TC3 cells high expressing PPAR γ _1 had no significant change ( P > 0.05 ) .

而PPARγ1高表达βTC3细胞的细胞活力无明显改变（P>0.05）。

The Effect of Glibenclamide on the K ~ + - ATP Channels of Pancreatic β TC3 with the Pretreatment of Amylin

胰淀素对优降糖作用于胰岛βTC3细胞ATP敏感性钾通道的影响

Methods Response of glucose stimulating insulin secretion ( GSIS ) and intracellular insulin content of rat islet cell and mouse β Tc3 cells were measured by radioimmunoassay .

方法应用放免法检测不同浓度葡萄糖培养后大鼠胰岛细胞和小鼠βTc3细胞在葡萄糖刺激时培育上清液和细胞内的胰岛素水平；

The ultrastructural and cytochemical analysis showed that NF9 and TC3 could enwind and infect the Phytophthora hyphae especially in the hypha within the medium .

超微结构与细胞化学研究表明，木霉菌丝能够缠绕并寄生疫霉菌菌丝，且重寄生作用主要发生在培养基内的菌丝上。

Conclusion ( 1 ) Sustained high glucose concentration was toxic to rat islet cells and mouse β Tc3 cells , as manifested by the increasing percentage of apoptotic cells and dysfunction of GSIS and decreasing synthesis of insulin ;

结论（1）长期高糖培养可以诱导大鼠胰岛细胞和小鼠βTc3细胞凋亡和功能缺陷；