taq enzyme

This article emphasizes particularly on the effect of Taq enzyme on the result of PCR quantitative detect .

本文重点探讨Taq酶对PCR定量检测结果的影响。

During the action of PCR , Taq enzyme has an important effect on the result of PCR quantitative detect .

在PCR反应过程中，Taq酶对PCR定量检测结果起到很大的影响。

Basic knowledge of analytical reagents & alkalis PCR reaction system , Taq enzyme , primer et al .

PCR反应体系、Taq酶、引物，Tris碱及聚丙烯酰胺等；

The satisfactory Taq enzyme amount is 1U ;

以1U酶量效果最满意；

A DDRT-PCR system was established by perfecting the conditions of Mg ~ ( 2 + ), annealing temperature , template concentration and Taq enzyme .

通过对退火温度、模板用量、Taq酶和Mg~（2+）浓度的优化，建立了适合多枝赖草基因差异显示的DDRT-PCR体系。

Screening ISSR-PCR the template concentration , primer concentration , dNTP concentration and Taq enzyme dosage , optimizing ISSR-PCR system . 4 .

分别筛选ISSR-PCR扩增的模板浓度、Taq酶用量、引物浓度及dNTP浓度，优化ISSR-PCR扩增体系。

Taq enzyme concentration 2U ;

Taq酶用量为2U；

The multiplex amplification conditions such as the concentration of primer , Mg2 + , Taq enzyme , anneal temperature , cycle times , and the time of pre-denaturation , final extension were optimized .

调节各基因座引物浓度、Mg2+浓度、Taq酶浓度、退火温度、循环次数、预变性时间、延伸时间等条件对复合体系进行优化，得到最佳的反应条件。

Results For LCN template DNA , if only increased the amount of Taq enzyme or the reaction volume , the results of STR amplification were scarcely improved , but if increased PCR cycles , the detection sensitivity were significantly improved .

结果对低拷贝模板DNA，单纯增加Taq酶量或反应体系，扩增效率改善不明显；增加循环数，显著提高检验灵敏度；

The result indicates that the ( amplification ) capability of Tgo enzyme is the same as that of Taq enzyme . But the amplification fidelity of Tgo enzyme is over 30 times higher than that of Taq enzyme and 1.6 times higher than that of Pfu .

该酶的扩增性能与Taq酶相当，而扩增忠实性分别比Taq酶、Pfu酶高30倍和1.6倍。

These factors included : the concentrations of Taq polymerase enzyme , MgCl 2 , template , dNTPs , primer , amplification of paired primers and thermal cycles .

这些因素包括：Taq酶、MgCl2、模板、dNTPs、引物浓度，双引物扩增及热循环数。

The results show that after of drug action , Taq estriction enzyme eLectrophoresis changed significantly . Show that Drugs and DNA binding sites o may be in two enzymes restriction site T / CGA .

结果表明DNA与药物作用后，Taq酶切电泳图发生明显改变，说明药物与DNA的结合位点可能在两种酶的酶切位点T/CGA上。

The amplification capability , the heat tolerance and the ability to long PCR of Tgo DNA polymerase were examined . The results reveal that Tgo DNA polymerase has the same amplification function as Taq ( enzyme ) but with a high fidelity .

检测了重组Tgo酶的扩增性能、耐热性以及长距离PCR能力，结果表明，该重组酶扩增性能与Taq酶相当；