However , the T320 → I mutation due to close to TAP 's active sites did bring about a significant descendents of the mutant in both the highest tolerable temperature and the optimal reaction temperature .
T320→I靠近酶的活性中心，尽管未能引起结构的较大变化，但却使最高耐受温度和最适反应温度同时显著降低。
t320