shRNA

Objective To explore effect of mismatched short hairpin RNA ( shRNA ) on reversing the multidrug resistance of leukemia cell .

目的探讨碱基错配对短发夹RNA（shRNA）逆转白血病细胞耐药作用的影响。

Constructed Eukaryotic Expression Plasmid of Small Hairpin RNA ( shRNA ) of PCNA and Investigated the Inhibitory Effect on Hela Cell

PCNA的shRNA的真核表达载体的构建及对子宫颈癌细胞的干预性研究

This study was to explore inducing effect of bcl-xL short hairpin RNA ( shRNA ) on apoptosis of CNE-2Z cells .

本研究拟探讨bcl-xL短发夹状RNA（ShorthairpinRNA，shRNA）诱导CNE-2Z细胞凋亡的作用。

Short hairpin RNA ( shRNA ) can be automatically processed into siRNA in cell and is more stable and effective than siRNA .

短发夹样RNA（ShorthairpinRNA，shRNA）在细胞内会自动被加工成siRNA，使基因沉默，且比siRNA作用更稳定，高效。

Objective To investigate the effect of short hairpin RNA ( shRNA ) targeting hypoxia-inducible factor-1 α( HIF-1 α) on the human breast carcinoma MCF-7 cell line .

目的研究靶向针对缺氧诱导因子-1α（HIF-1α）的短发夹RNA（shRNA）对人乳腺癌细胞系MCF-7细胞功能的影响。

Objective : To generate eukaryotic expression vector of short hairpin RNA ( shRNA ) specific for Y-box binding protein-1 ( YB-1 ) .

目的：构建Y-盒结合蛋白1（Y-boxbindingprotein-1，YB-1）特异性短发夹环RNA（shRNA）真核表达载体。

[ Objective ] To establish and identify the plasmid expressive vector coding short hairpin RNA ( shRNA ) targeting epidermal growth factor receptor ( EGFR ) of human gene .

目的构建靶向表皮生长因子受体（EGFR）的短发卡状RNA（shRNA）质粒表达载体并进行鉴定。

Objective : To study the effect of short hairpin RNA ( shRNA ) on the expression of human telomerase reverse transcriptase ( hTRT ) and telomerase activity in PC-3M cells .

目的：研究短发夹状RNA（shRNA）对PC3M细胞中端粒酶逆转录酶（hTRT）基因表达及端粒酶活性的影响。

OBJECTIVE : To investigate the effect of short hairpin RNA ( shRNA ) targeting CD_ ( 44 ) V_3 on CD_ ( 44 ) V_3 expression and proliferation ability of human lung carcinoma cell line A549 in vitro .

目的：研究针对CD44V3的短发夹RNA（ShorthairpinRNA，shRNA）对体外培养的人肺腺癌A549细胞CD44V3表达及增殖能力的影响。

As one form of RNAi , short hairpin RNA ( shRNA ) is composed of both sense and antisense sequences with a few bases ( 19-21nt ) which are reverse repeat sequences named as loop between them .

短发夹状RNA（ShorthairpinRNA，shRNA）由短的正义链、反义链（19-21nt）和连接它们的数个碱基组成，正义链、反义链呈反向重复序列，因其形似发夹而得名。

Objective To construct a plasmid vector expressing the short hairpin RNA ( shRNA ) targeting proliferation cell nuclear antigen ( PCNA ), and to investigate its effect in vitro on the expression of PCNA , proliferation and apoptosis of human osteosarcoma cells .

目的构建增殖细胞核抗原（PCNA）短发夹状RNA表达载体，检测其对人骨肉瘤细胞PCNA表达、细胞增殖及细胞凋亡的影响。

Objective To study the inhibitory effects of osteopontin ( OPN ) - specific small hairpin RNA ( shRNA ) on the expression of osteopontin ( OPN ) and its effect on the proliferation of in vitro cultured vascular smooth muscle cells in rats .

目的应用特异性短发夹RNA（shRNA）抑制血管平滑肌细胞骨桥蛋白基因表达，观察其对体外培养的血管平滑肌细胞增殖的影响。

This study was to establish an orthotopic animal model bearing human bladder cancer , monitor tumor progression by magnetic resonance imaging ( MRI ), and observe the synergistic effect of survivin short hairpin RNA ( shRNA ) in combination with MMC for intravesical treatment using this animal model .

本研究目的是建立荷人膀胱癌的原位动物模型，通过磁共振成像（MagneticResonanceimaging，MRI）监测肿瘤生长过程，并利用此模型评价靶向Survivin的干扰质粒对丝裂霉素的增效作用。