rtpcr

Development of Rapid Real-Time RTPCR Method for Detection of Mumps Virus

流行性腮腺炎病毒荧光定量逆转录-聚合酶链反应快速检测方法的建立

Finally , two differentially regulated genes were validated by real time RTPCR .

定量RTPCR验证芯片结果中两个差异表达基因。

Amplification of Dengue Virus Type 1 RNA by RTPCR and Its Clinical Application

RT-PCR扩增Ⅰ型登革病毒核酸及其临床应用

The RTPCR Expression Analysis on Sox Gene of Different Tissues from the Trionyx Sinensis

中华鳖不同组织Sox基因表达的RT&PCR分析

The detection method for various enteroviruses in water sample by RTPCR with universal primers was established .

建立了一种利用通用引物RT-PCR技术检测水中肠道病毒的方法。

The Detection of PFK Gene Transcripts by Nested RTPCR in Early Embryos of Kunming Mouse

昆明小鼠早期胚胎PFK基因转录产物的检测

Real-time quantitative PCR ( RtPCR ) is regarded as the most accurate and sensitive methods for rapidly detecting pathogens in various samples .

实时荧光定量PCR被认为是目前检测病原体的最快速的高特异和高敏感的新技术，已开始用于病毒、细菌等病原体的快速检测。

Aim and Methods : To study the effect of ventricular fibroblasts increase beating rate on cultured neonatal rat cardiomyocytes by using cell culture and RTPCR technique .

目的和方法：利用细胞培养和RTPCR技术研究新生大鼠心脏成纤维细胞增加心肌细胞搏动频率的作用。

Detection of IBV in Chickens by RTPCR

RT-PCR检测禽传染性支气管炎病毒

The silencing effects of TIMP-1 on the mRNA and protein levels were examined by reverse transcription PCR ( RTPCR ) and Western blot analysis respectively .

采用RT-PCR和Western印迹测定宿主细胞TIMP-1在基因和蛋白水平的沉默效果。

The transgenic efficiency was evaluated by Southern-blotting , RTPCR , Fibrin-agarose plate assay ( FAPA ) and Western-blotting . Results suggested that bio-active human t-PA was successfully expressed in the transgenic tobacco system .

通过对转化植株进行southernblotting，RT-PCR，WESTERNBLOTTING和纤维蛋白琼脂糖平板（fibrin-agaroseplateassay，FAPA）法检测，结果显示，在转基因烟草中成功地表达出有活性的人t-PA。

In the present paper , the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR , and Sox gene fragments of expression from the testicle , brain and spleen were cloned using RTPCR products .

本文采用RT&PCR技术，研究了中华鳖不同组织Sox基因的表达，并通过PCR直接克隆法，分析了来自睾丸、脑和脾组织中的Sox基因序列。

Present study observed the direct effects of hypoxia on expression of inducible nitric oxide synthetase ( iNOS ) in endothelial cells ( CMEC ) which isolated from rats'cerebral cortical microvessels , using reverse transcription polymerase chain reaction ( RTPCR ) technique .

未研究采用反转录多聚酶链反应技术观察了低氧对培养的大鼠大脑皮层微血管内皮细胞中诱导型一氧化氮合成酶基因表达的影响。

One of the foot-and-mouth disease virus ( FMDV ) type Asia 1 endemic strain nonstructural protein P3 coding region was amplified by RTPCR and sequenced . P3 coding regions of the virus and other representative reference strains were compared for analysis of gene characteristics .

经RT-PCR扩增得到一株口蹄疫亚洲1型流行毒株的非结构蛋白P3基因核苷酸序列，与其他代表性参考毒株的P3基因进行比较，分析该毒株P3区基因特征。