replicon

Novel in vitro cell culture models for HCV : Replicon systems

HCV的新型体外细胞模型：复制子系统

Each segment of DNA thus synthesized is called a replicon .

这样合成的每一片段DNA称为复制子。

Development of Vaccine Research Derived from JEV Replicon Expression Vector System

基于日本脑炎病毒复制子载体系统的疫苗研究

Research Development of RNA Replicon - based Vaccines

RNA复制子疫苗研究进展

Subgenomic replicon model of hepatitis C virus and it 's application

丙型肝炎病毒非结构基因复制子模型的建立和应用

Construction of Replicon Expression Vector Derived from XJ - 160 Virus

XJ-160病毒复制子型表达载体的构建

Construction and Application of Subgenomic Replicon Vectors of Japanese Encephalitis Virus

日本脑炎病毒（JEV）复制子表达载体的构建及其鉴定

The Construction of GHRH Gene Expression Vector Based on RNA Replicon and Its Expression in 293 Cell

基于RNA复制子GHRH基因表达载体的构建及在293细胞中的表达

These discoveries make it possible for the development of selectable full length genome replicon .

这些研究结果为选择性HCV全序列基因组复制子的研究奠定了基础。

Expression of SS gene in the muscle in mice by improved RNA-based replicon vector

含RNA复制子表达载体介导的生长抑素基因在小鼠肌肉中的表达

Construction of RNA Replicon Vaccine and DNA Vaccine of HBV and Comparison of Their Immune Efficiency in Mice

乙肝病毒RNA复制子疫苗、DNA疫苗的构建及其对小鼠免疫效果的比较

A Modified Replicon Expression Vector Derived from RNA Replicon of Semliki Forest Virus

一种基于塞姆利基森林病毒复制子的新型复制子载体

Investigating the inhibitory effects of interferon - α on the replication of hepatitis C virus replicon

干扰素α对丙型肝炎病毒复制子的抑制作用

Selecting template and the binding of transcription factors with RNA pol to form replicon are two major regulatory means .

选择模板、RNA聚合酶与转录因子结合形成复制体是两种主要的调控方法。

RNA Replicon - based Vaccines

RNA复制子疫苗

Construction and Identification of Replicon Vector Derived from an Infectious Full-length cDNA Clone of a Sindbis Virus

辛德毕斯病毒复制子载体系统的构建

High-level Expression of Foreign Genes In vivo and In vitro by Improved DNA-Based Replicon Vector Derived From Semliki Forest Virus

基于DNA的Semliki森林病毒复制子载体体内外高水平表达外源基因

Alphavirus Replicon - vectored Plasmid DNA - based Vaccine Elicits Protective Immunity Against Classical Swine Fever Virus

基于甲病毒复制子载体的猪瘟DNA疫苗的免疫效力评价

The results indicated that the replicon vector derived from XJ-160 virus was self-replicating and that the following gene expression was efficient .

结果表明我们构建的XJ-160病毒复制子型表达载体具有自主复制功能，可以表达异源基因。

Napp has little cytotoxic effect on target cells and it can effectively inhibit the RNA level of HCV subgenomic replicon cell model .

体外实验Napp对靶细胞安全作用范围较宽，且可有效抑制HCV复制子RNA。

HCV replicon and infectious cell models not only can be applied in many aspects of drug evaluation and screening , but also the research of HCV pathogenesis .

HCV复制和感染细胞模型不仅应用于药物评价、筛选方面，而且还极大地促进了HCV致病机制方面研究。

Development of a Eukaryotic Expression Vector Derived from Semliki Forest Virus RNA Replicon and a Derivative RNA Vaccine Candidate Against Type 2 Porcine Circovirus Infections

基于SemlikiForest病毒RNA复制子的表达载体及其衍生的2型猪圆环病毒RNA疫苗的初步研究

The improved DNA-based replicon vectors enhance the utility of them , and can be developed as potentially replicon vaccines and gene therapy vectors .

该复制子载体增强了原复制子载体应用能力，并有潜力作为复制子疫苗和基因治疗载体。

Even if containing a species-specific replicon , the plasmids can only be transformed into quantity-limited species or quantity-limited strains of Bifidobacterium .

即使是含有特异复制子的质粒也只能转化有限数量种型甚至有限数量种株的双歧杆菌；

All results demonstrated that the DNA-based WNV replicons could express exogenous genes and replicate itself well . This may set a foundation for following observation of replicon immunogenicity . 2 .

这些结果说明，DNA形式的西尼罗病毒复制子能够表达外源基因，具有良好的复制活性，为下一步观察该复制子的免疫原性奠定了基础。

[ Objective ] To explore the improved preparation of protoporphyrin disodium , observe the cytotoxicity of Napp in vitro and its inhibitory effect on HCV subgenomic replicon cell model .

目的本研究对原卟啉钠的制备方法进行了改进，以期简化其生产流程。并且体外观察原卟啉钠的细胞毒性作用及其对HCV亚基因复制子细胞模型RNA的抑制效果。

The 5 ′ translation enhancing region of the Semliki forest virus ( SFV ) capsid protein gene improves antigen expression and immunogenicity of SFV replicon based HIV Gag DNA vaccine

塞姆利基森林病毒翻译增强序列对HIVGagDNA疫苗抗原表达和免疫原性的调节

Objective To investigate the effects of 5 ′ translation enhancing region of Semliki forest virus ( SFV ) capsid protein gene on the gene expression and immunogenicity of HIV Gag DNA vaccine based on SFV replicon DNA vector .

目的研究塞姆利基森林病毒（Semlikiforestvirus，SFV）衣壳蛋白5′翻译增强区对基于该病毒复制子的HIVGagDNA疫苗抗原表达水平和免疫原性的影响。

Objective To construct a novel eukaryotic expression vector based on Semliki forest virus ( SFV ) replicon and express the DNA vaccine containing subtype core protein p24 and multi-epitope MEG chimeric gene of epidemic strain B of HIV-1 in China .

目的利用塞姆利基森林病毒（SFV）复制子构建新型真核表达载体，并对含HIV-1中国流行株B亚型核心蛋白p24及多表位MEG嵌合基因的核酸疫苗进行表达与鉴定。

Conclusion Antigen expression and cellular immune responses induced by the HIV Gag DNA vaccine based on a SFV replicon DNA vector were significantly improved by fusing the translation enhancing region of SFV capsid gene to the 5 ′ terminus of gag gene .

结论SFVC蛋白翻译增强区能显著提高SFV复制子DNA疫苗的抗原表达和抗原特异性细胞免疫反应。