recombinant strain

Study on Carotenoids Biosynthesis of The Recombinant Strain

重组菌株合成类胡萝卜素的研究

Construction of the Bacillus Thuringiensis Recombinant Strain with Melanin Formation and Research on the Condition of Producing Melanin

产黑色素B.thuringiensis重组菌株的构建及其培养条件的优化

Determine the expression level of fusion protein and test for the biochemical property of the recombinant strain .

提取不同代次的质粒，扫描电镜观察，检测融合蛋白表达水平，并进行工程菌的染色镜检及生化鉴定。

Results : Recombinant strain E.

结果：表达工程菌株E。

Development of a High-yield Recombinant Strain for Xylanase Production and Characterization of the Recombinant Enzyme

高效木聚糖酶基因工程菌的构建及重组酶酶学性质与功效研究

Conclusion A recombinant strain suitable for the rapid and large - scale production of Glucagon was constructed .

结论获得分泌胰高血糖素基因工程菌，适于快速大规模生产。

Advances on Cloning and Expression of Nitrile Hydratase Gene into the Recombinant Strain

腈水合酶基因克隆与调控表达的研究进展

After culture optimization , the enzyme production of the recombinant strain was 3 times higher than that of the wild strain .

优化培养条件后，此重组菌的发酵酶活单位明显高于野生菌，最高达野生菌的3倍。

The study on purification and characterization of HIV-1 reverse transcriptase from a recombinant strain of E.coli

重组人免疫缺陷病毒Ⅰ型逆转录酶的纯化及其动力学性质

The followed approach to analyze the properties of the recombinant strain , and used for surface display other functional foreign protein is under the way .

对重组菌性能的分析测定、以及进一步用于展示其它功能性外源蛋白的工作仍然在进行中。

We use the carbon monoxide differential spectrometry to determine the activity of VHb which is expressed by recombinant strain .

利用CO差光谱法对重组菌表达的透明颤菌血红蛋白进行了活性及定量检测。

Recombinant strain induced , the expressed products were SDS-PAGE , and the determination by DNS method mannan of the protein activity .

重组菌株经诱导表达，将表达产物进行SDS-PAGE，并用DNS法的测定该蛋白的甘露聚糖酶活性。

Bt recombinant strain and transgenic plant transformed with Bt genes have been regarded as the main measures for biological control of pests .

利用基因工程技术构建Bt工程菌和Bt转基因植物已成为对害虫进行生物防治的重要手段。

Genetic engineering methods is used to transform the strain , building high expressing xylose reductase recombinant strain , This can improve the yield of xylitol .

天然利用木糖生产木糖醇的菌株很多，但产率不高，一般通过基因工程手段改造菌株，构建高表达木糖还原酶的重组菌株，提高木糖醇产率。

After 10 passages , the recombinant strain still could express the target protein . The result indicated that the recombinant strain was stable in heredity .

所获重组菌株经连续传10代后仍能表达目的蛋白，表明其具有一定的遗传稳定性。

The variation of amino acids at the sites 286 and 799 may be the reasons of the transmission dominance of the B / C recombinant strain .

初步分析表明我国B/C重组毒株286位和799位氨基酸的变异可能为B/C重组株在我？

Conclusion : Application of the method is available of finding wild poliovirus , vaccine_derived poliovirus and recombinant strain , and preventing them from spreading .

结论：该方法应用于PV检测，可以尽早发现脊灰野病毒、疫苗衍生株及疫苗重组株等，便于尽快采取措施阻断其传播。

The result shows that the acid producing ability of recombinant strain without IPTG added is improved by 13 % , and also , the strain concentration is improved by 10.9 % .

结果显示，发酵过程中不加IPTG的重组菌的菌浓比原始菌提高了10.9%，产酸能力提高了13.0%。

Aim at the problem of organic chlorides in pulping effluents , a protoplast fusion experiment was carried out and a recombinant strain was constructed with an ability of degrading chlorophenols efficiently .

针对废水中含有大量有机氯合物，进行原生质体融合实验，构建可高效降解有机氯化物的重组菌。

Two and 10 days after recombinant strain intragastric immunization , the C57BL / 6 mice were sacrificed , and the spleens and terminal ileums were cultured for recombinant strain .

重组菌C57BL/6小鼠喂灌，分批2d和10d后处死小鼠，取脾和末段回肠进行细菌培养，挑菌落提质粒鉴定。

In the fermentation broth of recombinant strain KGK , the proportion of Er-A was improved to 91.1 % , and titer of Er-A also increased by 18.6 % .

重组菌的发酵液中Er-A的比例可达91.1%，且Er-A产量提高了18.6%。

Recombinant strain were induced by IPTG . Though SDS-PAGE , the expressed products were fusion protein by 62 KD band , indicating that the Aspergillus niger β - mannanase successful expression in E. coli .

重组菌株经IPTG诱导表达，进行SDS-PAGE，在菌体裂解物上清得到62KD的融合蛋白条带，表明黑曲霉β-甘露聚糖酶在大肠杆菌中表达成功。

This recombinant strain , and another bacterial strain that constitutively expresses HCV core antigen were orally inoculated BALB / c mice respectively . The stability of the plasmid in the bacteria and the immune responses was analyzed .

将该重组菌和组成性表达的重组菌分别口服接种BALB/c小鼠，观察质粒的稳定性和小鼠的免疫应答。

The special protein band about 44 kD was shown in the SDS-PAGE gel . The glucose isomerase activity of recombinant strain was 18.562 U / mg under optimal conditions and 3 times as much as that of wild-type strain .

重组菌经诱导培养，SDS-PAGE电泳结果显示出明显的分子量约为44kD特异性蛋白质条带，比酶活约为18.562U/mg，比野生型菌株提高了2倍。

The Efficacy of Recombinant Strain Expressing the Major Protective Antigens of Shiga-like Toxigenic Escherichia Coli Causing PWD / ED on Trial and Genotyping of PWD / ED Suspected Pigs Bearing F18 Fimbria Receptors

表达致PWD和ED大肠杆菌保护性抗原基因的重组菌的中间试验、环境释放试验及我国部分养殖猪种F18受体基因型的检测

Objective : To establish a recombinant strain over-expressing catalytic region of glucosyltransferase ( GTF-I , containing B-Cell epitope ) from Streptococcus sobrinus 6715 for the study of anti GTF-I monoclonal antibody and the sub-unit of anti-caries vaccine .

目的：构建一株高效表达Streptococcussobrinus6715中GTF-I催化活性区（含有B细胞表位）多肽的菌株，为抗GTF-I的单克隆抗体的检测和防治龋病亚单位疫苗的研究奠定基础。

Study on fermentation for recombinant bacterial strain of human interleukin-6

基因重组人IL-6工程菌发酵培养的实验研究

Construction of Recombinant Yeast Strain Using Cellobiose as Sole Carbon Source

利用纤维二糖的酵母工程菌构建

Influence of Culture Temperature on Growth Density of Recombinant Bacterial Strain and Expression of rhG-CSF

培养温度对基因工程菌生长密度和rhG-CSF表达的影响

Microsatellite DNA Monitoring Cultivation of Recombinant Inbred Strain of Mice

微卫星DNA监控近交系小鼠的重组近交系培育研究