Methods Different dosages of Radis Astragali were added in the mediums for fibroblast culture . The ICAM-1 and CD126 positive cells were assessed by using flow cytometry .
方法采用细胞培养法,于细胞培养液中加入不同浓度的药物,通过流式细胞仪观察经分子探针标记的ICAM1、CD126阳性细胞数变化,用放免法测定培养上清HA、LA含量。