protein isoelectric point

The protein isoelectric point was 7.29 , no disulfide bonds . 2 .

该蛋白等电点为7.29，无二硫键。

The treatment of thermal denaturation , the pH closing to the protein isoelectric point and adding calcium can lead to decrease of the value of circular dichroism signal of collagen . It indicates that these factors can affect the behavior of collagen molecules in solution .

热变性处理、接近蛋白等电点pH以及添加钙离子均可导致胶原的圆二色信号值减弱，说明这些因素均会影响胶原蛋白分子的溶液行为。

Bioinformatics analysis indicated that the mutations in structural domain might lead to the changes of the amino acid sequence coded , second structure of protein , isoelectric point and antigen binding site .

生物信息学分析发现编码区的突变可导致氨基酸序列、蛋白质的二级结构及等电点、抗原结合位点等发生改变。

As revealed by SDS_PAGE , the 59.5 kD and 63.5 kD proteins were the major components of the microfibrillar protein purified by isoelectric point precipitation .

SDSPAGE分析表明，经等电点纯化的微纤维蛋白质的主要成分是59.5kD和63.5kD蛋白质。

With biological software analysis , obtained cDNA sequence base composition , codon usage and restriction site data , as well as detailed theoretical protein molecular weight , isoelectric point and amino acid composition .

利用生物软件进行分析，获得cDNA序列碱基组成、密码子使用频率和酶切位点等详细数据以及蛋白质理论分子量、等电点和氨基酸组成等情况。

The starch was removed by α - amylase at high-temperature , the protein was removed by isoelectric point precipitation method , and the β - glucan was purified by alcohol precipitation method .

采用高温α-淀粉酶去除淀粉，等电点沉淀蛋白，醇沉法将β-葡聚糖进行纯化。

The results indicated that beer and beer foam had quite similar protein molecular weight distribution ( both composed by 43 kD and 7-17 kD protein ), and protein molecular weight distribution and protein isoelectric point distribution in beer foam were smaller than that in beer .

结果表明：啤酒和啤酒泡沫蛋白的分子量分布相似，均由43kD和7-17kD的蛋白组成；在分子量和等电点的分布上啤酒泡沫蛋白的分布要少于啤酒蛋白的分布；