phagemid

Construction of Naive Mouse Phagemid Single Chain Antibody Library

天然鼠源噬菌体单链抗体库的构建

Insert sequences in positive phage clones were then transformed to phagemid and sequenced and analyzed to decided to further study or not .

筛选到的阳性克隆通过噬粒转化、核苷酸测序、生物信息学分析，确定要深入研究的对象。

The low digestion efficiency of the PCR products brought a disadvantage result that the cloning efficiency of the PCR products directly into the phagemid was very low .

山于PCR产物的酶切效率低，所以直接将PCR产物酶切后克隆入表达载体的效率是非常低的。

Phage antibody library is a repertoire of antibodies , which connects human antibody genes to phagemid vector and is expressed in bacteria .

噬菌体抗体库（phageantibodylibrary）是将人抗体基因与噬菌粒载体相连，在细菌体内表达而制备的人全套抗体（repertoireofantibodies）。