pff

Current Application of pFF to in Vitro Fertilization of Porcine Ovarian Oocytes

猪卵泡液在猪体外受精中的应用

It analyses the motivation of the PFF program from 3 aspects which refer to the duty , quality and demand of college teachers .

对PFF项目开展的动因，笔者分别从高校教师的职责、高校教师的素质和高校教师的需求三方面进行了分析。

In this investigation , we study the effect of season , FCS , pFF , insulin and estradiol on pig oocytes in vitro maturation ( IVM );

本实验系统研究了猪卵母细胞的体外成熟、冷冻保存、体外受精及早期胚胎发育，着重研究了季节因素、FCS、pFF、胰岛素和17β-E2等对猪卵母细胞体外成熟的影响；

This research aim to establish an efficient system for producing pigs by somatic cell nuclear transfer ( SCNT ) . Effect of hormone 、 pFF and EGF on maturation of porcine oocytes have been investigated .

本研究旨在建立一套高效的体细胞克隆猪的生产体系。研究了激素、猪卵泡液及表皮生长因子对猪卵母细胞体外成熟的影响。

After classification the oocytes were cultured for 36 hours in TCM-199 containing pFF or PMSG , in humidified atmosphere of 5 % CO2 CO2 and 95 % of air at 39 ℃ .

分类后卵母细胞在含有pFF或PMSG的TCM&199培养液中于5%CO＿2、95%空气、饱和湿度、39℃下培养36小时。

The objective of the present study was to examine the effects of porcine follicular fluid ( pFF ) and fetal calf serum ( FCS ) on in vitro maturation of porcine oocytes derived from small follicles ( diameter < 2 mm ) .

本研究探讨了猪卵泡液（pFF）和胎牛血清（FCS）对猪小腔卵泡卵母细胞体外成熟的影响。

Since 1993 , Association of American Colleges and Universities and the Graduate School Committee co-sponsored the " Preparing Future Faculty " project ( PFF ), according to the fact that it is difficult to make the doctoral education adapt to the requirements of new teachers in University .

从1993年开始，针对博士生教育难以适应高等教育对大学新教师的要求，美国学院与大学联合会和研究生院委员会共同发起了未来师资培训规划项目（PFF）。

The effects of oocyte-collecting method ( aspiration , dissection , mechanical break-up ), in vitro oocyte maturation culture method ( plate culture , droplet culture ), and porcine follicular fluid ( PFF ) concentration in culture medium to porcine oocyte culture and in vitro fertilization was compared .

比较了卵母细胞采集方法（剖解法，抽吸法，机械破碎法）、卵母细胞体外成熟培养方法以及猪卵泡液（PFF）质量浓度对猪卵母细胞培养和体外受精的影响。

The addition of pFF to the develpment medium enhanced the development of porcine embryos in culture , increasing the proportion of the embryos passing through the 4-cell block from 3.9 % ( 6 / 154 ) to l0.2 % ( 12 / 118 ) .

在培养基中添加pFF可促进猪胚胎体外发育，使发育至8～16－细胞期的胚胎率从3.9%（6／154）提高到10.2%（12／118）。