p32

Separation and Purification of the surface protein P32 . 7 of N.

bombycis的蛋白质尤其是表面蛋白为研究对象，对其进行分离纯化及其相关领域的分析研究。

Construction and expression of recombinant antigen P32 gene of goat poxvirus

山羊痘病毒P32基因真核表达载体的构建及表达

Studies on Some Biological Characteristics and P32 Gene Encoding for the Main Structural Protein of Goat Pox Virus

山羊痘病毒某些生物学特性及其主要结构蛋白P32基因的研究

The results showed that P32 and CD58 were successfully expressed in BHK-21 cells .

结果显示，P32和CD58在BHK-21细胞中都得到表达。

Cloning , Sequencing and Expression of Goat Poxvirus P32 Gene

山羊痘病毒P32基因克隆、测序及表达

Cloning and sequence analysis of P32 surface protein genes of three strains of bovine Theileria sergenti

三株牛瑟氏泰勒虫P32表面蛋白基因的克隆与序列分析

Cloning of the Major Surface Protein P32 Gene from Ovine Theileria and Construction of Eukaryotic Expression Vector

羊泰勒虫主要表面蛋白P32基因的克隆及真核表达载体的构建

Cloning of the gene of capripoxvirus major antigen P32 and construction of its baculovirus expression vector

羊痘病毒P32蛋白基因的克隆及其杆状病毒表达载体的构建

Development of an indirect ELISA for detection of antibodies against Goat pox virus using recombinant p32 protein as antigen

山羊痘病毒p32基因原核表达及间接ELISA抗体检测方法的建立

Finally , the expressed product from the P32 gene was detected by SDS-PAGE and Western-blotting .

最后用SDS-PAGE和Western-blotting对P32基因的表达进行检测。

Expression of P32 gene of goatpox virus in baculovirus

山羊痘病毒P32基因在杆状病毒中的表达

The TK and P32 gene deleted transfer vectors were constructed , and they would be used in homologous recombinant ;

以TK基因或P32基因为目的基因，构建基因缺失转移载体用于同源重组；

P32 protein is one of the virus structure proteins . It stands out on the virus envelope surface and contains neutralized antigen epitope .

羊痘病毒P32蛋白是位于羊痘病毒膜表面的主要结构蛋白，含有中和抗原表位；

Expression of Capripoxvirus P32 Protein

羊痘病毒P32蛋白的表达

The following work were done in this study : ( 1 ) TK and P32 gene of GTPV-TY were cloned by PCR with primer pairs designed by information published on GENBANK ;

主要进行了以下工作：（1）根据GENBANK公布的羊痘病毒基因组序列设计引物，利用PCR克隆GTPV-TY株TK基因和P32基因；

Establishment of PCR method and sequence analysis for P32 gene : According to the sequences of P32 gene in GenBank , a pair of specific primers were designed , and a PCR method was established successfully .

基于P32基因PCR方法的建立及其基因序列分析：参照GenBank已发表的GPV基因序列，针对P32基因设计与合成了1对特异性引物，成功地建立了针对P32基因的PCR方法。

Seven cases of new karyotypes of chromosome abnormalities were found which were not reported previously in the world . 46 ,, XX , t ( 1 ; 7 ) ( P32 ; P22 );

本文首报7例世界人类染色体异常核型如下：46，XX，t（1；7）（p32；p22）；

After plasmid purification , the acquired recombinant plasmids were transfected into BHK-21 cell with liposome mediated method . the expression of P32 and CD58 were tested by direct immunofluorescence assay ( IFA ) or indirect immunofluorescence assay .

质粒提纯后，在脂质体作用下转染BHK-21细胞，通过直接免疫荧光试验和间接免疫荧光试验分别检测P32和CD58在BHK-21中的表达。

The medium for cultivation of fluorescent pseudomonads strain P32 was studied . According to the tests , the optimum medium for its growth consists of sugar 6 % , glycerol 3 % , peptone 1 % , ( NH_4 ) H_2PO_40.5 % .

本文对荧光假单胞菌P32的培养基成分进行了研究，利用正交试验筛选出产菌量较高的培养基，成分如下：（%，W/V）蔗糖6，甘油3，蛋白胨1，NH4H2PO4,0.5。

A pair of PCR primes were designed according to the published sequence of capripoxvirus major antigen p32.The P32 gene was multiplied by PCR and cloned into the pMD-18-T vector , we got the recom - binant bacmid pMD-p32.Then the P32 gene was cloned into the E.

根据发表的羊痘病毒P32基因，设计一对特异性引物，PCR扩增P32全长基因，将其克隆入pMD-18-T载体，获得重组质粒pMD-P32。