p32
- 网络第32页
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Separation and Purification of the surface protein P32 . 7 of N.
bombycis的蛋白质尤其是表面蛋白为研究对象,对其进行分离纯化及其相关领域的分析研究。
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Construction and expression of recombinant antigen P32 gene of goat poxvirus
山羊痘病毒P32基因真核表达载体的构建及表达
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Studies on Some Biological Characteristics and P32 Gene Encoding for the Main Structural Protein of Goat Pox Virus
山羊痘病毒某些生物学特性及其主要结构蛋白P32基因的研究
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The results showed that P32 and CD58 were successfully expressed in BHK-21 cells .
结果显示,P32和CD58在BHK-21细胞中都得到表达。
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Cloning , Sequencing and Expression of Goat Poxvirus P32 Gene
山羊痘病毒P32基因克隆、测序及表达
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Cloning and sequence analysis of P32 surface protein genes of three strains of bovine Theileria sergenti
三株牛瑟氏泰勒虫P32表面蛋白基因的克隆与序列分析
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Cloning of the Major Surface Protein P32 Gene from Ovine Theileria and Construction of Eukaryotic Expression Vector
羊泰勒虫主要表面蛋白P32基因的克隆及真核表达载体的构建
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Cloning of the gene of capripoxvirus major antigen P32 and construction of its baculovirus expression vector
羊痘病毒P32蛋白基因的克隆及其杆状病毒表达载体的构建
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Development of an indirect ELISA for detection of antibodies against Goat pox virus using recombinant p32 protein as antigen
山羊痘病毒p32基因原核表达及间接ELISA抗体检测方法的建立
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Finally , the expressed product from the P32 gene was detected by SDS-PAGE and Western-blotting .
最后用SDS-PAGE和Western-blotting对P32基因的表达进行检测。
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Expression of P32 gene of goatpox virus in baculovirus
山羊痘病毒P32基因在杆状病毒中的表达
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The TK and P32 gene deleted transfer vectors were constructed , and they would be used in homologous recombinant ;
以TK基因或P32基因为目的基因,构建基因缺失转移载体用于同源重组;
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P32 protein is one of the virus structure proteins . It stands out on the virus envelope surface and contains neutralized antigen epitope .
羊痘病毒P32蛋白是位于羊痘病毒膜表面的主要结构蛋白,含有中和抗原表位;
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Expression of Capripoxvirus P32 Protein
羊痘病毒P32蛋白的表达
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The following work were done in this study : ( 1 ) TK and P32 gene of GTPV-TY were cloned by PCR with primer pairs designed by information published on GENBANK ;
主要进行了以下工作:(1)根据GENBANK公布的羊痘病毒基因组序列设计引物,利用PCR克隆GTPV-TY株TK基因和P32基因;
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Establishment of PCR method and sequence analysis for P32 gene : According to the sequences of P32 gene in GenBank , a pair of specific primers were designed , and a PCR method was established successfully .
基于P32基因PCR方法的建立及其基因序列分析:参照GenBank已发表的GPV基因序列,针对P32基因设计与合成了1对特异性引物,成功地建立了针对P32基因的PCR方法。
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Seven cases of new karyotypes of chromosome abnormalities were found which were not reported previously in the world . 46 ,, XX , t ( 1 ; 7 ) ( P32 ; P22 );
本文首报7例世界人类染色体异常核型如下:46,XX,t(1;7)(p32;p22);
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After plasmid purification , the acquired recombinant plasmids were transfected into BHK-21 cell with liposome mediated method . the expression of P32 and CD58 were tested by direct immunofluorescence assay ( IFA ) or indirect immunofluorescence assay .
质粒提纯后,在脂质体作用下转染BHK-21细胞,通过直接免疫荧光试验和间接免疫荧光试验分别检测P32和CD58在BHK-21中的表达。
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The medium for cultivation of fluorescent pseudomonads strain P32 was studied . According to the tests , the optimum medium for its growth consists of sugar 6 % , glycerol 3 % , peptone 1 % , ( NH_4 ) H_2PO_40.5 % .
本文对荧光假单胞菌P32的培养基成分进行了研究,利用正交试验筛选出产菌量较高的培养基,成分如下:(%,W/V)蔗糖6,甘油3,蛋白胨1,NH4H2PO4,0.5。
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A pair of PCR primes were designed according to the published sequence of capripoxvirus major antigen p32.The P32 gene was multiplied by PCR and cloned into the pMD-18-T vector , we got the recom - binant bacmid pMD-p32.Then the P32 gene was cloned into the E.
根据发表的羊痘病毒P32基因,设计一对特异性引物,PCR扩增P32全长基因,将其克隆入pMD-18-T载体,获得重组质粒pMD-P32。