nopaline

Determination on nopaline dehydrogenase from tumor callus of soybean plants caused by

大豆属致瘤农杆菌致瘤后胭脂碱的测定

The nopaline synthetic enzyme activity of the preliminary transformation was detected by electrophoresis .

取转化苗叶片作胭脂碱合成酶活性检测电泳后呈阳性；

Teratoma Induction and the Nopaline Synthase Gene Transfer in Peanut

花生畸胎瘤冠瘿瘤的诱导及胭脂碱合成酶基因的转移

A_ ( 84 ) sensitivity determined by both nopaline type Ti plasmid and its host cell

A（84）的敏感性与胭脂碱Ti质粒及宿主的关系

A number of transgenic plants were obtained using the plasmid containing a sweet protein NPT ⅱ and nopaline synthase genes .

把带有甜蛋白基因和胭脂碱合成酶标记基因的Ti质粒导入马铃薯，获得大量转化植株。

Transient expression vectors were constructed by fusing the promoter fragment with gus reporter gene and nopaline terminator in different orientation .

将启动子片段分别以不同方向与gus报告基因和nos终止子融合，构建了瞬时表达载体。

The detection of nopaline in the crown galls showed that T_DNA of Ti plasmid had been transformed successfully .

栝楼冠瘿组织经除菌后能在无激素的MS培养基上良好生长，纸电泳检测结果表明其合成了冠瘿碱，表明Ti质粒转化成功。

Examining each parts of the plants separately , nopaline was present in all leaves and crown galls but was not detected in the roots .

分别测试了植株的各部分，植株的叶及其冠瘿都含有nopaline，而根中未测出nopaline。

It was concluded that using the nopaline type of Agrobacterium ( e.g.C58 ), 5min vacuum infiltration , and using the plants with just several blooming flowers would give the best results .

其中以开花初期的植株，利用胭脂碱型农杆菌介导（如C58），真空渗入5分钟的处理方法转化效率最高。

The duplex PCR detection results of Agrobacterium tumefaciens nopaline synthase ( nos ) terminator and Escherichia coli strain K12 neomycin phosphotransferase II ( npt ⅱ) gene in these samples were all negative .

应用根癌农杆菌胭脂碱合成酶基因（nos）终止子和大肠杆菌K12菌株新霉素磷酸转移酶Ⅱ（nptⅡ）基因的二重PCR对样品进行转基因成分检测，结果均为阴性。