morulae

Comparison of Efficacy Between Two Vitrification Solutions for Mouse Morulae

两种玻璃化液冻存小鼠桑椹胚的研究

Study by Ultrarapid Freezing Method and Evaluation of Viability of Rabbit Morulae

家兔桑椹胚超速冷冻法研究及胚胎存活力的判断

A Simple Method for Cryopreservation of Mouse Compacted Morulae by Vitrification

小鼠桑椹胚简易玻璃化冷冻技术再探讨

Study on the developing potential of mouse morulae produced in vitro or in vivo after vitrification

体内、体外产生的小鼠桑葚胚玻璃化冻存后的发育能力

Effect of Bio-Frequency Spectrum on Development of Mouse Vitrified Morulae in vitro

小鼠桑椹胚玻璃化冷冻前后生物频谱处理对体外发育的影响

Attachment of the Rabbit Morulae and Early Blastocysts on the Feeder Layer

家兔桑椹胚和早期囊胚在饲养层表面的贴壁行为

Effects of Bio Frequency Spectrum on Development of Mouse Morulae after Vitrification in Vitro and in Vivo

生物频谱对小鼠桑椹胚玻璃化冷冻后体内外发育影响的研究

Osmotic and cryoprotective effect of glycerol sucrose solutions on mammalian morulae

甘油蔗糖液对哺乳动物桑椹胚的渗透反应及冻存效果研究

Ninety five nuclear transplanted morulae and blastocysts were transferred to 33 synchronous recipients .

将发育到桑椹胚和囊胚期的95枚核移植胚胎移植到33头受体牛，其中31头受体牛在移植后第2个情期前返情；

Developmental capacity of mechanically bisected mouse morulae and blastocysts

机械分割的小鼠桑椹胚和囊胚的发育能力

Sexing of Mouse Morulae Vitrified by OPS Method with Diplex , Nested PCR

双重套式PCR对小鼠桑椹胚OPS法冷冻后性别鉴定

The optimal conditions of embryonic cell fusion and oocyte activation and enucleation to improve nuclear transplantation in the rabbit 32 - cell morulae were extensively studied .

本文就兔卵母细胞去核、胚胎细胞融合与卵母细胞激活等条件，对提高兔桑椹胚细胞核移植技术效率进行了进一步研究。

However , the morulae / blastocyst formation rate of fibroblasts was significantly lower than cumulus granulosa cells and oviduct epithelial cells after fused ( P0.05 ) .

但在融合后的桑椹胚/囊胚形成率上，成纤维细胞又明显低于卵丘颗粒细胞和输卵管上皮细胞（P0.05）。

Rates of fertilization and morulae / blastocysts in oocytes with fully expanded cumulus were significantly higher than those in oocytes with poorly expanded cumulus .

扩展良好的山羊卵巢卵母细胞的受精率和胚胎发育能力显著高于卵丘扩展差的卵母细胞。

Under this dehydration condition , 73 68 % and 80 56 % of rabbit morulae developed to blastocysts in vitro after ultrarapid freezing .

在这种脱水状态下将桑椹胚进行超快速冻存，解冻后有73.68%或80.56%在体外发育至囊胚。

42 morulae and 13 blastocysts were frozen in 1 M glycerol / PBS in the RPE freezer .

用RPE冷冻器冷冻42枚桑椹胚和13枚囊胚。

The above results showed that OPS vitrified mouse morulae could be successfully diluted and transfered by in-straw method , and it will provide a model for ET in small ruminant .

证明OPS玻璃化冷冻的小鼠桑椹胚可以进行管内解冻和直接移植，为小家畜胚胎移植提供了模型。

In comparison with bisection of frozen-thawed morulae , bisection of frozen-thawed blastocysts in sucrose solution can improve survival rate of demi-embryos ( P < 0.05 ) .

与分割解冻桑椹胚相比，在蔗糖液中分割解冻囊胚可显著提高冻胚分割效果（P<0.05）。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated , and the possible roles of fibronectin in fertilization , the development of early embryo and implantation were also discussed .

并观察FN在人卵母细胞和早期胚胎内的表达和分布，以了解FN在受精、早期胚胎发育和植入过程中的可能作用。

Two stage embryos were observed their effect on production of chimera . The result shows that there is a significant different ( 0.01 P 0.05 ) between blastocysts ( including early blastocysts and morulae ) and expanded blastocysts .

结果表明，囊胚、早囊及桑椹胚同扩张囊胚两种注射胚胎制作嵌合体所产生的结果差异显著（0.01＜P＜0.05）。

26.9 % , 13.0 % , 48.8 % and 29.2 % of the 2 - , 4 - , 8-cell embryos and morulae developed normally after micromanipulation , respectively . 8 cell stages show the best development in vitro .

2、4、8细胞卵和桑椹胚在显微分割后，分别有26.9%、13.0%、48.8%和29.2%的半胚正常发育，其中8细胞卵的半胚在体外的发育结果最好。

A total of 45 goats , 42 Guanzhong dairy goats and 3 Boer goats , were superovulated with a result of total 341 embryos including 26 embryos of 4-cell to 16-cell , 144 morulae , 108 blastocysts and 63 hatched blastocysts .

超排处理45只山羊（42只关中奶山羊，3只波尔山羊），共得到可用胚胎341枚，其中4～16细胞胚胎26枚，桑椹胚144枚，囊胚108枚，孵化胚63枚。

The demi-embryos development rates of yeanling group ( including morulae 、 blastocyst ) were higher than multiparity sheep group ( 68 . 8 % vs68 . 2 % x 85 . 7 % vs81 . 8 % , P > 0.05 ) .

育成羊组（含桑葚胚、囊胚）分割胚的囊胚发育率都高于经产羊组，但差异不显著（68.8%vs68.2%、85.7%vs81.8%，P>0.05）。

The experiment showed that the culture system of SOF + 10 % FCS facilitates the cleavage of parthenogenetic porcine embryos . NCSU-23 supplemented with FCS and granulosa cell improved the development of porcine parthenogenetic embryos and the rate of morulae / blastocysts ( P < 0.05 ) .

试验表明：孤雌激活卵母细胞在SOF+10%FCS培养体系中分裂效果最好，添加胎牛血清的NCSU-23和颗粒细胞对胚胎发育有促进作用，培养6d后发育到桑囊胚的比率增加（P<0.05）。

The results indicated that : The blastocyst rates of demi-embryos in 0 . 2molS + PBS group ( including morulae 、 blastocyst ) were higher than PBS group ( 68 . 2 % vs58.3 % x 81 . 8 % vs80 . 0 % , P0 . 05 ) ;

结果表明，0.2molS+PBS分割液组（含桑葚胚、囊胚）的二分胚囊胚发育率高于PBS分割液组，但差异不显著（68.2%vs58.3%、81.8%vs80.0%，P0.05）；