morula

Increasing the Efficiency of Nuclear Transplantation in the Rabbit Morula

提高兔桑椹胚细胞核移植技术效率的研究

Results showed that the pregnant rate of blastula was higher than that of morula .

结果表明，囊胚较桑椹胚妊娠率高；

Some factors affecting the cryopreservation of mouse morula by vitrification method were been studied .

探讨了用玻璃化法冷冻保存小鼠桑椹胚的一些影响因素。

Embryos from fetal fibroblast could only develop to morula stage with the rate of 0.2 % ;

胎儿成纤维细胞重构胚最高发育阶段为桑椹胚，比例为02%；

The Leveraging of the Rich Morula is rich in nutrient components and has a high value of utilization .

桑椹营养物质含量丰富，利用价值高，在食品和医药保健方面开发前景广阔。

Mice morula rate of parthenogenetic embryos in ethanol plus feeder cells group was highest 80.38 % ;

小鼠孤雌胚的桑椹胚率乙醇加饲养层组最高80.38%；

The survival rate of early blastocysts was significantly higher than that of the morula ( P0.05 ) . 6 .

经统计分析，早期囊胚的存活率显著高于桑椹胚（P＜0.05）。

There was no significant difference between control and treatments for morula and early blastocyst treated with different glycerol concentration . 5 .

经统计分析，对照组与不同浓度甘油组之间小鼠桑椹胚和早期囊胚的存活率均差异不显著。

In morula , positive LN immunoreactive substance appeared simultaneously in both blastomere and extracellular matrix .

到桑椹胚期，卵裂球内及间质中同时出现LN阳性反应物质。

Morula / blastocyst development rate of sheep embryos from activation was improved significantly by adding 3.0 % NEAA .

添加3.0%NEAA能显著提高绵羊孤雌激活胚胎桑囊胚的发育率。

The rabbit embryos at stages of 2 cell , 4 cell , 8 cell and morula were frozen with vitrified methods .

将兔2细胞，4细胞，8细胞以及桑椹期的胚胎快速冷冻。

There was no significant difference in lambing rate per embryo from the morula stage to blastocyst ( P > 0.05 ) .

胚胎成羔率从桑椹胚到囊胚差异不显著（P>0.05）。

This experiment collected 109 embryos to observe their developmental ability by ES cells microinjection into 8-16 cell stage morula or blastocyst .

本试验先后将109枚胚胎用于此项研究，通过将小鼠ES细胞注入8-16细胞桑椹胚和囊胚，观察两个不同时期注射胚胎的发育能力。

The rates of cleavage and morula activated by 6-DMAP + ethanol were the highest among all metheds used .

本实验所有激活方法中，6DMAP+乙醇激活法所得卵裂率和桑椹胚率最高。

The results are as follows : ( 1 ) The mitochondria in the embryos before morula stage were mostly hooded .

结果表明：（1）在桑椹期以前，胚胎中大都为带帽线粒体，从此期开始这种线粒体明显减少；

Objective To compare the survival and developmental potential of mouse morula , early blastocysts and blastocysts cryopreserved by vitrification .

目的比较玻璃化法冻融小鼠桑葚胚期、囊胚早期和囊胚期胚胎后，胚胎存活及继续发育的能力。

Establishment of the mouse morula embryo cryobank

小鼠桑椹期胚胎库的建立

In the experiment , 30 A - and B - grade morula and blastula were selected for sexing .

试验选择体内受精生产的发育级别为A级和B级的桑椹胚，使用玻璃切割针进行切割。

The studies were performed on the cryopreservation of mouse morula by vitrification , embryo splitting and embryo transfer ( ET ) .

本实验对小鼠桑椹胚的玻璃化冷冻保存、胚胎分割及胚胎移植技术进行了系统的研究。

Number of normal and abnormal embryo of treated group In Morula and Blastocysts Stages had great significance difference comparing with corresponding control group ( P < 0.01 ) .

桑葚胚、囊胚时期实验组正常胚胎、异常胚胎数量与对照组相比，具有极显著差异（P<0.01）。

The survival rates of the early blastocyst and morula treated with 2.5M glycerol were 98.2 % and 73.7 % respectively .

小鼠桑椹胚和早期囊胚用2.5M甘油处理存活率分别为73.7%和98.2%。

The proportion of morula and blastocysts of cumulus cells was 25 % , fibroblasts was 32.7 % .

桑葚胚／囊胚率：颗粒细胞为25%，成纤维细胞为32.7%；

The experiment showed that the sampling periods for calculating the egg-fertilizing rate are clearage stage , whereas , morula stage is best .

本试验通过对四大家鱼鱼卵受精率计算方法的初步研究，得出如下结论：（1）计算家鱼鱼卵受精率的取样时间为卵裂期，尤以桑椹期为佳。

No expression was detectable in 4-cell embryos , 8-cell embryos , morula and blastocysts .

而在4细胞胚、8细胞胚、桑椹胚和囊胚中不表达。

With regard to embryo quality the pregnant rate of morula for grade A ( 50.4 % ) was significantly higher than that of grade B ( 38.3 % ) .

胚胎质量以A级桑椹胚妊娠率最高，为50.4%，显著高于B经桑椹胚38.3%的妊娠率（P<0.05）；

The survival rates of rabbit early blastocyst and morula were 93.8 % and 63.3 % , respectively , when the glycerol was 2.5M .

2.5M甘油溶液对不同发育阶段胚胎存活率的影响实验中，兔早期囊胚和桑椹胚的存活率分别为93.8%、70.0%。

When the morula cut into half - embryos were transfered , the success rate was 21.1 % while that of the early developing blastocysts came to 28.6 % .

桑椹胚分割成半胚移植怀孕成功率为21.1%，而早期囊胚则为28.6%。

In vitro cultured early embryos , Tb4 mRNA began to increase from the zygotes stage , until the morula and blastula stages , the expression reached a peak .

在早期胚胎体外培养的过程中，Tb4mRNA从合子时期开始出现上调性表达，直到桑椹胚和囊胚，其表达量达到峰值。

Most embryos from natural estrous rats migrated into the uterus ahead of morula stage , while embryos from superovulated rat entered the uterus at 8-cell stage .

自然发情大鼠的胚胎多在近桑椹胚期进入子宫，而超排大鼠在8-细胞期即已进入子宫。

The survival rates of both early blastocyst and morula treated with 0.5M trehalose were significantly higher than that of 0.25M trehalose treated ( P < 0.05 ) .

经统计分析，桑椹胚和早期囊胚在0.5M海藻糖溶液中的存活率均显著高于0.25M组（P＜0.05）。