m13

Experiments of Mammalian Genetic Engineering Experiment 14 M13 Cloning and DNA Sequencing

实验14M13克隆和DNA顺序分析

M13 bacteriophage has been extensively applied in phage surface display technique .

M13噬菌体已被广泛地应用于噬菌体展示技术。

In the current study , M13 p ⅲ based phage display random peptide libraries were constructed .

本课题中，我们构建了M13噬菌体pⅢ蛋白融合的表面展示随机肽文库。

Genetic analysis suggests that a single recessive gene was responsible for the M13 phenotype .

遗传分析表明M13受单基因隐性控制。

Belcher and her team took a harmless virus called M13 .

贝尔彻和她的研究小组采用一种名叫M13的无害病毒来作实验。

DNA SEQUENCE DETERMINATION The M13 Dideoxynucleotide Sequencing System

DNA序列测定&M13双脱氧核苷酸序列分析系统

DNA Fingerprinting in Pig Produced by Minisatellite Probe from Bacteriophage M13

用M13小卫星探针发现猪DNA指纹

The purified PCR products were cloned into pGEM-T vector and then sequenced using M13 forward primers .

PCR产物纯化后克隆到PGEMT载体，并用引物M13Forward测序。

Fluconazole resistance in Candida albicans assayed by PCR fingerprinting with M13 primer

应用M13引物PCR指纹法分析假丝酵母菌氟康唑耐药性

Objective : To construct a library of M13 PIII fusion displaying peptides composed of 20 random amino acids .

目的：构建一个随机20个氨基酸的丝状噬菌体肽库。

Objective : To explore the feasibility of expressing a fusion protein with ⅰ - ⅱ domain of M13 phage P ⅲ protein .

目的：探讨M13噬菌体PⅢ蛋白Ⅰ-Ⅱ结构域表达融合蛋白的可行性。

Virus templates in virus-enabled rechargeable battery mainly deal with M13 bacteriophage virus and tobacco mosaic virus .

病毒基二次电池的病毒模板主要研究了M13噬菌体病毒以及烟草花叶病毒。

Hirudin Display on the Surface of Bacteriophage M13

水蛭素在噬菌体表面的展示

Deletion of Cloned DNA Fragment in M13 and Application of Bluescript Vector for Subcloning and Sequencing

DNA序列分析中克隆片段在M13的缺失及质粒载体的应用

Neprilysin ( NEP ) is a type II integral membrane glycoprotein of the M13 zinc metalloprotease family .

Neprilysin（NEP）是M13锌金属蛋白酶家族的一种Ⅱ型整合细胞膜糖蛋白。

The designed deletion was completed by means of the U-containing system and phage M13 system , with efficiency of 6 % and 1 % respectively .

在192个单核苷酸缺失突变中，含U单链基因突变系统和经典噬菌体M13单链突变系统均准确完成缺失突变，两种方法的突变年率分别为6%和1%左右。

At the1974 dedication of Arecibo Observatory , a radio message about Earth was sent in the direction of M13 .

1974年，阿雷西波天文台向M13方向发送了一份关于地球的无线电讯息。

The successful display of hirudin on the surface of M13 phage laid a sound foundation for the further study on directed evolution of antithrombotic proteins with altered properties .

水蛭素在噬菌体表面的功成展示为进一步开展其实验定向进化以及结构与功能关系的研究打下基础。

Phage M13 with 12-polypeptides displayed on its surface was used to prepare the phage-displayed protein chip and the real time process of interaction between this 12-polypeptides and the corresponding antibody was monitored .

以展示有12肽的噬菌体M13作为探针，制备了噬菌体展示蛋白质芯片，并实时检测了该12肽与其相应抗体相互作用的过程，实验结果表明SPR传感可检测活体上的分子反应。

The purified RSV was biotinylated in vitro with NSH LC biotin and then to react with random peptide library displaying 7 amino acids fused on protein ⅲ of M13 phage .

病毒用生物素标记后，应用M13噬菌体PⅢ呈现随机7肽库进行筛选。

Methods Amplification of exon 2 of DRB gene in this heterozygote was carried out with genomic DNA templet and generic primers and the amplicon was cloned in M13 for further sequencing .

方法用DRB基因类引物扩增该特殊的DRB1杂合子细胞基因组的DRB基因的第二外显子。扩增产物经纯化转染大肠杆菌JM101，克隆后再经酚／氯仿抽提后得到单链M13DNA。

Objective : To clone the extracellular domain ′ s gene of human CD20 and N1-domain ′ s gene of g3p of filamentous phage M13 and express the fusion protein high efficiently in Escherichia coli .

目的：克隆人CD20胞外区基因（cdw）和丝状噬菌体（M13K07）G3蛋白N端结构域（pⅢN1）基因，并在大肠杆菌中进行高效融合表达。

Bacteriophage M13 was used for cloning the RAPD marker linked to the Seedless genes in grapevine , fluorescence based specific DNA sequences 484 bp were obtained by an automated fluorescent DNA sequencer systems ( Model 373A Version 2.0 ) .

利用细菌质粒M13克隆葡萄无核基因的RAPD标记UBC-269450后，采用自动荧光DNA序列分析仪对其测序。结果表明，UBC-269450由484对核苷酸及其特定序列构成。