keratinase

The Research Situation and Prospect of keratinase Application

角蛋白酶应用研究现状及展望

Research advances in molecular biology of microbial keratinase

微生物角蛋白酶的分子生物学研究进展

Medium Optimization for Keratinase Production in Feather Substrates

以羽毛为底物发酵产角蛋白酶培养基的优化

Extraction , Cloning and Expression of Keratinase Gene kerB

角蛋白酶基因kerB的提取、克隆及表达

Isolation , Identification of Feather Keratin Degrading Bacteria and Cloning , Expression of Keratinase Gene

羽毛角蛋白质降解菌的分离、鉴定与角蛋白酶基因的克隆、表达

Purification , characterization and application of keratinase from Bacillus sp. l_4 stain

芽孢杆菌L4所产角蛋白酶的纯化工艺、理化性质及其应用研究

Breeding of keratinase - producing strain by fusion of non - auxotroph protoplast

非营养缺陷型原生质体融合选育角蛋白酶生产菌

The progress of studies on keratin , keratinase and keratin degrading microorganisms were reviewed .

本文对角蛋白、角蛋白酶、角蛋白降解菌的研究和应用状况作了系统的综述。

Enzymatic Characteristics of Keratinase from Bacillus sp. L_4 Strains

芽孢杆菌L4菌株角蛋白酶的酶学性质研究

The crude keratinase had been extracted from the fermentation liquid of U3-22 strain , and the enzymatic characteristics were analyzed .

从U3-22菌株的发酵液提取粗角蛋白酶，分析其酶学性质。

Studies on the Genetic Diversity and Thermophilic Keratinase and Lipase of Thermophiles from Hot Springs in Tengchong Yunnan

云南腾冲热泉嗜热微生物的遗传多样性及嗜热角蛋白酶和脂肪酶的研究

Studies on feather-degrading Flavobacterium breve zdm , the fermentation conditions and characteristics of keratinase

羽毛角蛋白降解菌（FlavobacteriumbreveZDM）的分离、鉴定及其发酵产酶条件和酶学特性的研究

Study on the phenotyping of Trichophyton mentagrophytes Expression of Trichophyton Mentagrophytes Keratinase and Researches on Their Biological Roles

须癣毛癣菌表型的研究须癣毛癣菌角蛋白酶的表达及生物学作用

Expression of Trichophyton Mentagrophytes Keratinase and Researches on Their Biological Roles ; Pathogenesis of Trichophyton rubrum secreted metalloprotease

须癣毛癣菌角蛋白酶的表达及生物学作用红色毛癣菌金属蛋白酶Metalloprotease致病性研究

The keratinase may have no specific enzyme cut sites and it cuts the substrates into peptides in different length . This showed a new enzyme cut style and it may have further foreground .

该角蛋白酶可能没有特异性的酶切位点，而是把底物切成肽段，该现象展示了此蛋白酶新的酶切方式，该种新酶切方式的研究意义非常远大。

In the batch culture , the optimal temperature was 30 ℃ and optimal pH was 9 . Ca ~ ( 2 + ) and Mg ~ ( 2 + ) were found to stimulate the keratinase activity .

在分批培养中，最适初始pH值为9，最适温度30℃，钙离子和镁离子对其酶活性有促进作用。

790U / mg respectively . Conclusion Purified human keratins is a fine substrate for inducing keratinase production . Specific activity of keratinase induced by purified keratin is higher than that induced by human hair .

结论：纯化人角蛋白是诱导角蛋白酶产生的良好底物，纯化人角蛋白诱导角蛋白酶比活性高于毛发诱导的角蛋白酶。